Desmocollin 1 (DSC1) (NM_024421) Human Untagged Clone
CAT＃: SC305114
DSC1 (untagged)-Human desmocollin 1 (DSC1), transcript variant Dsc1a
CNY 17200.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CDHF1; DG2/DG3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC305114 representing NM_024421.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCTCTGGCCTCTGCTGCCCCAGGGAGCATCTTCTGTAAGCAGCTCCTTTTCTCTCTCCTGGTTTTA ACATTACTTTGCGATGCTTGTCAGAAAGTTTATCTTCGAGTTCCTTCTCATCTTCAGGCTGAAACACTT GTAGGCAAAGTGAATCTGGAGGAGTGTCTCAAGTCGGCCAGCCTAATCCGGTCCAGTGACCCTGCCTTC AGAATTCTAGAAGATGGCTCAATTTACACAACACATGACCTCATTTTGTCTTCTGAAAGGAAAAGTTTT TCCATTTTCCTTTCAGATGGTCAGAGACGGGAACAACAAGAGATAAAAGTTGTACTGTCAGCAAGAGAA AACAAGTCTCCTAAGAAGAGACATACCAAAGACACAGCCCTCAAGCGCAGCAAGAGACGATGGGCTCCT ATTCCAGCTTCATTGATGGAGAACTCGTTGGGTCCATTTCCACAACACGTTCAGCAGATCCAATCTGAT GCTGCACAGAATTACACCATCTTTTATTCCATAAGTGGGCCAGGCGTGGACAAAGAACCCTTCAATTTG TTTTACATAGAGAAAGACACTGGGGATATCTTTTGTACAAGGAGCATTGACCGTGAGAAATATGAACAG TTTGCGTTATATGGCTATGCAACAACTGCAGATGGCTATGCACCAGAATATCCACTCCCTTTGATCATC AAAATTGAAGATGATAATGATAACGCCCCATATTTTGAACACAGAGTGACTATCTTTACTGTGCCTGAA AATTGCCGATCCGGAACTTCAGTGGGAAAAGTGACCGCCACAGACCTTGACGAACCTGACACTCTCCAT ACTCGTCTGAAATATAAAATCTTACAACAAATCCCAGATCATCCAAAGCATTTCTCCATACACCCAGAT ACCGGTGTCATCACCACAACTACACCTTTTCTGGATAGAGAAAAATGTGATACTTACCAGTTAATAATG GAAGTGCGAGACATGGGTGGTCAGCCTTTCGGTTTATTTAATACAGGAACAATTACTATTTCACTTGAG GATGAAAATGACAATCCACCATCTTTCACAGAAACTTCTTATGTTACAGAAGTAGAAGAAAACAGAATT GACGTGGAGATTTTACGAATGAAGGTACAGGATCAGGATTTGCCAAACACTCCTCACTCAAAGGCTGTA TACAAAATCCTACAAGGAAATGAAAATGGAAACTTCATAATTAGCACAGATCCAAATACAAATGAAGGA GTGCTGTGTGTTGTCAAGCCATTGAACTATGAAGTCAATCGCCAAGTTATTTTGCAAGTTGGTGTCATT AACGAGGCACAATTCTCTAAAGCAGCGAGCTCACAAACTCCTACAATGTGCACTACAACTGTCACCGTT AAAATTATAGACAGTGATGAGGGCCCTGAATGCCACCCTCCAGTGAAAGTTATTCAGAGTCAAGATGGC TTCCCAGCTGGCCAAGAACTCCTTGGATACAAAGCACTGGACCCGGAAATATCCAGTGGTGAAGGCTTA AGGTATCAGAAGTTAGGGGATGAAGATAACTGGTTTGAAATTAATCAACACACTGGCGACTTGAGAACT CTAAAAGTACTAGATAGAGAATCCAAATTTGTAAAAAACAACCAATACAATATTTCAGTTGTTGCAGTG GATGCAGTTGGCCGATCTTGCACTGGAACATTAGTAGTTCATTTGGATGATTACAACGATCACGCACCT CAAATTGACAAAGAAGTGACCATTTGTCAGAATAATGAGGATTTTGCTGTTCTGAAACCTGTAGATCCA GATGGACCTGAAAATGGACCACCTTTTCAATTCTTTCTGGATAATTCTGCCAGTAAAAACTGGAACATA GAAGAAAAGGATGGTAAAACTGCCATTCTTCGTCAACGGCAAAATCTTGATTATAACTATTATTCTGTG CCTATTCAAATAAAAGACAGGCATGGTTTAGTTGCAACACATATGTTAACAGTGAGAGTATGTGACTGT TCAACTCCATCTGAGTGTAGAATGAAGGATAAAAGTACAAGAGACGTTAGACCAAATGTAATACTTGGA AGATGGGCTATTCTTGCTATGGTGTTGGGTTCTGTATTGTTATTATGTATTCTGTTTACATGTTTCTGT GTCACTGCTAAGAGAACAGTCAAGAAATGTTTTCCAGAAGACATAGCCCAGCAAAATTTAATTGTATCA AATACTGAAGGACCTGGAGAAGAAGTAACGGAAGCAAATATTAGACTCCCCATGCAGACATCCAACATT TGTGACACAAGCATGTCTGTTGGTACTGTTGGTGGCCAGGGAATCAAAACACAGCAAAGTTTTGAGATG GTCAAAGGAGGCTACACTTTGGATTCCAACAAAGGAGGTGGACATCAGACCTTGGAGTCCGTCAAGGGA GTGGGGCAGGGAGATACTGGCAGATATGCGTACACGGACTGGCAGAGTTTCACCCAACCTCGGCTTGGC GAAAAGGTGTATTTGTGTGGACAAGATGAGGAGCATAAACATTGTGAAGACTACGTTTGTTCGTATAAC TATGAAGGCAAAGGTTCTCTGGCCGGCTCAGTAGGTTGCTGCAGCGATCGGCAGGAAGAAGAGGGACTG GAGTTTCTAGATCACCTGGAACCCAAATTTAGGACATTAGCAAAGACATGCATCAAGAAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_024421 |
| Insert Size | 2685 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_024421.2 |
| RefSeq Size | 4225 bp |
| RefSeq ORF | 2685 bp |
| Locus ID | 1823 |
| UniProt ID | Q08554 |
| Protein Families | Transmembrane |
| MW | 100 kDa |
| Gene Summary | The protein encoded by this gene is a calcium-dependent glycoprotein that is a member of the desmocollin subfamily of the cadherin superfamily. These desmosomal family members, along with the desmogleins, are found primarily in epithelial cells where they constitute the adhesive proteins of the desmosome cell-cell junction and are required for cell adhesion and desmosome formation. A subtype of IgA pemphigus, a life-threatening autoimmune disease, is characterized by the presence of autoantibodies that target the encoded protein. The desmosomal family members are arranged in two clusters on chromosome 18. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature protein. [provided by RefSeq, Nov 2015] Transcript Variant: This variant (Dsc1a) represents the shorter transcript and encodes the longer isoform (Dsc1a). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214491 | DSC1 (Myc-DDK-tagged)-Human desmocollin 1 (DSC1), transcript variant Dsc1a |
CNY 6552.00 |
|
| RC214491L3 | Lenti ORF clone of Human desmocollin 1 (DSC1), transcript variant Dsc1a, Myc-DDK-tagged |
CNY 9030.00 |
|
| RC214491L4 | Lenti ORF clone of Human desmocollin 1 (DSC1), transcript variant Dsc1a, mGFP tagged |
CNY 9030.00 |
|
| RG214491 | DSC1 (tGFP-tagged) - Human desmocollin 1 (DSC1), transcript variant Dsc1a |
CNY 7890.00 |
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