MARCHF4 (NM_020814) Human Untagged Clone
CAT＃: SC304840
41337 (untagged)-Human membrane-associated ring finger (C3HC4) 4 (MARCH4)
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MARCH-IV; MARCH4; RNF174 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC304840 representing NM_020814.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCTGATGCCCCTGTGTGGGCTGCTCTGGTGGTGGTGGTGCTGCTGCTCCGGCTGGTACTGCTATGGA TTGTGTGCCCCAGCCCCCCAGATGTTGCGCCACCAGGGTCTCCTCAAGTGCCGCTGCCGCATGCTCTTC AATGACCTGAAGGTTTTCTTACTGCGGCGCCCTCCTCAAGCGCCCCTGCCCATGCACGGCGACCCCCAG CCCCCCGGTTTGGCGGCCAACAACACCCTTCCGGCTCTGGGCGCCGGGGGGTGGGCAGGCTGGAGGGGC CCCCGAGAAGTGGTGGGCAGGGAGCCCCCTCCTGTGCCACCTCCACCCCCCTTGCCACCTTCTTCTGTG GAAGATGACTGGGGTGGCCCAGCCACAGAGCCACCTGCCTCGCTGCTCAGCAGTGCCTCCTCAGATGAC TTCTGTAAGGAGAAGACCGAGGATCGCTACTCACTGGGCAGCAGCTTGGACAGTGGTATGAGGACCCCA CTCTGCCGCATCTGCTTCCAGGGGCCAGAACAGGGGGAGCTGCTGAGCCCATGCCGCTGTGATGGCTCG GTCAAGTGCACACACCAGCCTTGCCTCATCAAGTGGATCAGCGAGCGGGGCTGCTGGAGCTGCGAGCTG TGCTACTACAAGTACCACGTCATCGCCATAAGCACAAAAAATCCTCTGCAGTGGCAGGCCATCTCTCTG ACGGTCATTGAGAAGGTTCAGGTTGCAGCCGCCATCCTGGGCTCCCTCTTCCTCATCGCCAGTATTTCT TGGCTCATCTGGTCAACTTTCAGCCCCTCGGCAAGATGGCAGCGCCAAGACCTTCTCTTCCAGATCTGC TACGGGATGTATGGCTTCATGGACGTGGTGTGCATAGGTCTCATCATCCATGAAGGACCCTCGGTGTAC CGCATCTTTAAACGGTGGCAGGCTGTCAACCAGCAGTGGAAAGTGCTGAACTATGACAAGACAAAAGAC CTGGAGGATCAAAAGGCAGGAGGCAGGACCAACCCCCGGACCTCCTCATCCACCCAGGCCAATATCCCC TCCTCGGAAGAGGAGACCGCAGGCACCCCTGCCCCTGAGCAGGGCCCTGCCCAGGCTGCCGGCCACCCC TCAGGCCCTCTGTCCCATCACCACTGTGCTTATACCATCCTGCACATCCTGAGTCACTTGAGACCTCAT GAACAGCGAAGTCCCCCAGGCAGCAGCCGAGAGCTGGTCATGAGAGTCACGACAGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_020814 |
| Insert Size | 1233 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020814.2 |
| RefSeq Size | 4466 bp |
| RefSeq ORF | 1233 bp |
| Locus ID | 57574 |
| UniProt ID | Q9P2E8 |
| Protein Families | Transmembrane |
| MW | 45.5 kDa |
| Gene Summary | MARCH4 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH4 reduces surface accumulation of several membrane glycoproteins by directing them to the endosomal compartment (Bartee et al., 2004 [PubMed 14722266]).[supplied by OMIM, Apr 2010] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212197 | MARCH4 (Myc-DDK-tagged)-Human membrane-associated ring finger (C3HC4) 4 (MARCH4) |
CNY 5488.00 |
|
| RC212197L3 | Lenti ORF clone of Human membrane-associated ring finger (C3HC4) 4 (MARCH4), Myc-DDK-tagged |
CNY 5890.00 |
|
| RC212197L4 | Lenti ORF clone of Human membrane-associated ring finger (C3HC4) 4 (MARCH4), mGFP tagged |
CNY 5890.00 |
|
| RG212197 | 41337 (tGFP-tagged) - Human membrane-associated ring finger (C3HC4) 4 (MARCH4) |
CNY 7088.00 |
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