ANKFY1 (NM_020740) Human Untagged Clone
CAT＃: SC304813
ANKFY1 (untagged)-Human ankyrin repeat and FYVE domain containing 1 (ANKFY1), transcript variant 2
CNY 7790.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ANKHZN; DKFZp686M19106; KIAA1255; ZFYVE14 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC304813 representing NM_020740.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGGAAGAGGAGGTGGCCAAGTTGGAGAAGCACTTGATGCTTCTGCGGCAGGAGTATGTCAAGCTG CAGAAGAAGCTGGCGGAGACAGAGAAGCGCTGCGCTCTCTTGGCTGCGCAGGCAAACAAGGAGAGCAGC AGCGAGTCCTTCATCAGCCGTCTGCTGGCCATCGTGGCAGACCTCTACGAGCAGGAGCAGTACAGCGAT CTGAAGATAAAGGTTGGGGACAGGCACATCAGTGCTCACAAGTTTGTCCTGGCAGCCCGCAGTGACAGC TGGAGTCTGGCTAACTTGTCTTCCACTAAAGAGTTGGACCTGTCAGATGCTAATCCTGAGGTGACGATG ACAATGCTTCGCTGGATCTATACAGATGAGCTGGAGTTCAGAGAGGATGATGTGTTCCTGACTGAACTG ATGAAACTAGCAAATCGGTTTCAGCTACAGCTCCTCAGGGAGAGATGTGAGAAGGGTGTTATGTCTCTA GTGAATGTCAGGAACTGTATTCGCTTCTACCAGACGGCAGAGGAGCTGAATGCCAGCACACTGATGAAC TACTGTGCAGAAATTATTGCAAGTCATTGGGACGACCTGAGGAAGGAGGATTTCAGCAGCATGAGCGCT CAGTTGTTATACAAAATGATCAAATCCAAGACAGAGTACCCGCTACATAAAGCCATCAAAGTGGAGAGA GAAGACGTGGTCTTCCTGTATCTGATTGAAATGGATTCCCAGCTCCCTGGGAAGCTGAATGAAGCGGAT CATAACGGAGATCTGGCATTAGATCTAGCCCTCTCACGACGACTGGAGAGTATTGCCACCACGCTGGTT AGTCACAAAGCTGATGTGGACATGGTGGACAAGAGTGGCTGGAGCTTGTTACACAAAGGAATCCAAAGA GGAGATCTCTTTGCTGCCACTTTCCTCATTAAGAATGGGGCCTTTGTCAACGCTGCTACACTGGGTGCC CAGGAGACACCACTGCACCTTGTGGCCTTGTACAGTTCAAAGAAACACTCAGCAGATGTGATGTCTGAG ATGGCGCAGATTGCAGAGGCCCTTCTGCAGGCTGGTGCCAACCCCAACATGCAGGACAGCAAGGGGAGG ACTCCTTTACATGTGTCCATCATGGCCGGGAATGAATATGTGTTCAGTCAGCTGCTGCAGTGCAAACAA CTAGATTTAGAACTCAAAGACCACGAGGGCAGCACGGCTCTGTGGCTGGCAGTGCAGCATATCACAGTG TCTTCTGACCAGTCTGTGAACCCCTTCGAAGATGTCCCCGTGGTAAATGGGACTTCATTTGATGAGAAC AGCTTTGCAGCCAGACTCATCCAGCGCGGCAGCCACACAGACGCACCTGACACGGCGACAGGTAAAGCA CGTGCCTCCCGAAGGGGCGACGCTGGGGTCTGCAGGCGCCAGGAAATGGCATGTAAATGCCTTCACCCC AAATTTAGAAACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_020740 |
| Insert Size | 1464 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020740.2 |
| RefSeq Size | 1927 bp |
| RefSeq ORF | 1464 bp |
| Locus ID | 51479 |
| MW | 54.8 kDa |
| Gene Summary | This gene encodes a cytoplasmic protein that contains a coiled-coil structure and a BTB/POZ domain at its N-terminus, ankyrin repeats in the middle portion, and a FYVE-finger motif at its C-terminus. This protein belongs to a subgroup of double zinc finger proteins which may be involved in vesicle or protein transport. Alternate splicing results in multiple transcript variants of this gene. [provided by RefSeq, Apr 2012] Transcript Variant: This variant (2) contains a distinct 3' UTR and 3' coding region, compared to variant 1. The resulting isoform (2) has a shorter, distinct C-terminus when compared to variant 1. Isoform 2 also lacks the FYVE-finger motif found in isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223110 | ANKFY1 (Myc-DDK-tagged)-Human ankyrin repeat and FYVE domain containing 1 (ANKFY1), transcript variant 2 |
CNY 3656.00 |
|
| RC223110L3 | Lenti ORF clone of Human ankyrin repeat and FYVE domain containing 1 (ANKFY1), transcript variant 2, Myc-DDK-tagged |
CNY 6750.00 |
|
| RC223110L4 | Lenti ORF clone of Human ankyrin repeat and FYVE domain containing 1 (ANKFY1), transcript variant 2, mGFP tagged |
CNY 6750.00 |
|
| RG223110 | ANKFY1 (tGFP-tagged) - Human ankyrin repeat and FYVE domain containing 1 (ANKFY1), transcript variant 2 |
CNY 5320.00 |
