Factor XI (F11) (NM_019559) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	coagulation factor XI; FXI; FXI, MGC141891; MGC141891; plasma thromboplastin antecedent; platelet coagulation factor XI
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_019559, the custom clone sequence may differ by one or more nucleotides ATGATTTTCTTATATCAAGTGGTACATTTCATTTTATTTACTTCAGTTTCTGGTGAATGTGTGACTCAGT TGTTGAAGGACACCTGCTTTGAAGGAGGGGACATTACTACGGTCTTCACACCAAGCGCCAAGTACTGCCA GGTAGTCTGCACTTACCACCCAAGATGTTTACTCTTCACTTTCACGGCGGAATCACCATCTGAGGATCCC ACCCGATGGTTTACTTGTGTCCTGAAAGACAGTGTTACAGAAACACTGCCAAGAGTGAATAGGACAGCAG CGATTTCTGGGTATTCTTTCAAGCAATGCTCACACCAAATAAGTAACATTTGTCTACTGAAGCACACCCA AACAGGGACACCAACCAGAATAACGAAGCTCGATAAAGTGGTGTCTGGATTTTCACTGAAATCCTGTGCA CTTTCTAATCTGGCTTGTATTAGGGACATTTTCCCTAATACGGTGTTTGCAGACAGCAACATCGACAGTG TCATGGCTCCCGATGCTTTTGTCTGTGGCCGAATCTGCACTCATCATCCCGGTTGCTTGTTTTTTACCTT CTTTTCCCAGGAATGGCCCAAAGAATCTCAAAGAAATCTTTGTCTCCTTAAAACATCTGAGAGTGGATTG CCCAGTACACGCATTAAAAAGAGCAAAGCTCTTTCTGGTTTCAGTCTACAAAGCTGCAGGCACAGCATCC CAGTGTTCTGCCATTCTTCATTTTACCATGACACTGATTTCTTGGGAGAAGAACTGGATATTGTTGCTGC AAAAAGTCACGAGGCCTGCCAGAAACTGTGCACCAATGCCGTCCGCTGCCAGTTTTTTACCTATACCCCA GCCCAAGCATCCTGCAACGAAGGGAAGGGCAAGTGTTACTTAAAGCTTTCTTCAAACGGATCTCCAACTA AAATACTTCACGGGAGAGGAGGCATCTCTGGATACACATTAAGGTTGTGTAAAATGGATAATGAGTGTAC CACCAAAATCAAGCCCAGGATCGTTGGAGGAACTGCGTCTGTTCGTGGTGAGTGGCCGTGGCAGGTGACC CTGCACACAACCTCACCCACTCAGAGACACCTGTGTGGAGGCTCCATCATTGGAAACCAGTGGATATTAA CAGCCGCTCACTGTTTCTATGGGGTAGAGTCACCTAAGATTTTGCGTGTCTACAGTGGCATTTTAAATCA ATCTGAAATAAAAGAGGACACATCTTTCTTTGGGGTTCAAGAAATAATAATCCATGATCAGTATAAAATG GCAGAAAGCGGGTATGATATTGCCTTGTTGAAACTGGAAACCACAGTGAATTACACAGATTCTCAACGAC CCATATGCCTGCCTTCCAAAGGAGATAGAAATGTAATATACACTGATTGCTGGGTGACTGGATGGGGGTA CAGAAAACTAAGAGACAAAATACAAAATACTCTCCAGAAAGCCAAGATACCCTTAGTGACCAACGAAGAG TGCCAGAAGAGATACAGAGGACATAAAATAACCCATAAGATGATCTGTGCCGGCTACAGGGAAGGAGGGA AGGACGCTTGCAAGGGAGATTCGGGAGGCCCTCTGTCCTGCAAACACAATGAGGTCTGGCATCTGGTAGG CATCACGAGCTGGGGCGAAGGCTGTGCTCAAAGGGAGCGGCCAGGTGTTTACACCAACGTGGTCGAGTAC GTGGACTGGATTCTGGAGAAAACTCAAGCAGTGTGA
Restriction Sites	Please inquire
ACCN	NM_019559
Insert Size	2000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_019559.1, NP_062505.1
RefSeq Size	3116 bp
RefSeq ORF	2055 bp
Locus ID	2160
Protein Families	Druggable Genome, Protease, Secreted Protein
Protein Pathways	Complement and coagulation cascades
Gene Summary	This gene encodes coagulation factor XI of the blood coagulation cascade. This protein is present in plasma as a zymogen, which is a unique plasma coagulation enzyme because it exists as a homodimer consisting of two identical polypeptide chains linked by disulfide bonds. During activation of the plasma factor XI, an internal peptide bond is cleaved by factor XIIa (or XII) in each of the two chains, resulting in activated factor XIa, a serine protease composed of two heavy and two light chains held together by disulfide bonds. This activated plasma factor XI triggers the middle phase of the intrisic pathway of blood coagulation by activating factor IX. Defects in this factor lead to Rosenthal syndrome, a blood coagulation abnormality. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) represents the shorter variant, compared to variant 1. The resulting isoform (b) is shorter than isoform a, encoded by variant 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.