HEATR5B (NM_019024) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC304652 representing NM_019024. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGTTAGCCCACAGTTTATTGCTAAATGAAGAAGCTTTGGCTCAAATCACCGAAGCAAAAAGACCA GTTTTCATCTTTGAATGGTTGCGATTTCTTGATAAAGTCTTGGTTGCTGCCAACAAGACCGATGTAAAG GAAAAACAGAAAAAACTTGTTGAACAATTAACTGGATTAATAAGTAGTTCACCTGGACCACCTACACGA AAATTATTAGCTAAAAATCTCGCAGCCCTTTATAGCATTGGGGATACTTTCACAGTTTTTCAGACACTT GATAAATGCAATGACATTATCAGAAATAAAGATGACACTGCGGCCTACTTACCAACAAAATTGGCTGCG GTGGCTTGTGTCGGAGCATTTTATGAAAAAATGGGGAGAATGTTGGGCAGCGCATTTCCAGAAACGGTA AATAATCTATTGAAATCTCTGAAAAGTGCAGAGTCTCAAGGGCGAAGTGAAATCTTAATGAGTCTACAG AAAGTTCTAAGTGGACTGGGTGGTGCAGCAGCTTCCTCCCATCGTGATATTTACAAGAATGCCAGGTCT CTCTTGACTGATAGGTCAATGGCTGTTCGATGTGCAGTGGCCAAGTGTCTACTAGAACTACAGAATGAA GCTGTATTTATGTGGACTGCTGAACTAGAAAATATAGCCACTCTCTGCTTTAAGGCTTTGGAAAACTCA AATTATGGGGTACGAGTGGCAGTGTCTAAACTTTTAGGAACAGTCATGGCCACAGCATTAATGCCAAAA CAGGCAACAGTAATGCGTCAGAATGTGAAGCGAGCAACATTTGATGAAGTCTTAGAACTCATGGCCACA GGATTTCTGCGTGGAGGGTCAGGTTTCTTAAAGAGCGGTGGAGAAATGTTAAAAGTTGGAGGGTCCGTT AATCGTGAAGTGAGAGTTGGAGTTACGCAGGCGTATGTTGTTTTTGTGACAACATTGGGTGGTCAGTGG TTGGAGCGCAGCTTTGCCACGTTCCTGTCCCATGTACTTGATCTGGTTTCCCATCCTCGGGCAACACAA ACACATGTGGAGGCTGTGTACTCCAGACGATGTGTTTCCTTTATCCTAAGAGCTACTGTGGGCAGTTTG CTAGGTGAAAAAGCCCAGATTGCAGCTGCCAAAGAAATCTGCCAAGCTATTGGAAAACAAATGAAAGCC GTAGAAGCAGTAGTGAATGACACAAGTGGAGAAAACAAATCTGGCGCAGCAGACATTGCAGCAAGCCAG CATGTGATGGTCTGTGCCCTCCAGGAACTCGGGAGCCTGGTGCAGAGCTTGAATGCCACCGCATCCCCT CTTATTCAAGAAGCATCTATAGGGCTTTTGGAGATTGTGACTTCAGTGCTGCTTCATCCAAGCATGGCT GCCCGACTTGCTGCTGCATGGTGTTTGCGCTGTGTGGCTGTGGCATTACCTTTCCAGCTGACACCATTT CTAGACAGGTGTGCAGAACGGCTCAACAACTTGAAGACCTCACCAGAAGCTGTCAGTGGATATAGTTTT GCAATGGCTGCTTTGTTAGGTGGAGTACATCAGTGTCCTTTGGGCATTCCTCATGCCAAAGGAAAGATG GTAGTTAGTATTGCTGAAGATCTTTTACGAACTGCTGCCCAAAATAGCAGGCTATCTTTACAGCGCACC CAAGCTGGCTGGCTTTTACTTGGAGCACTTATGACTTTAGGACCATCTGTCGTTCGTTACCATCTGCCC AAGATGTTGTTATTGTGGCGAAATGTTTTCCCACGTTCCTTAAAGGAATTGGAAGCTGAGAAGGCCCGA GGCGATTCTTTTACCTGGCAGGTAACTTTGGAAGGTCGTGCTGGAGCTCTATGTGCCATGAGGAGCTTC GTTGCACATTGTCCTGAGCTACTAACTGAAGATGTGATTCGAAAATTGATGACCCCTATTGAATGTGCC ATGACTATGATGTCACACATTCCATCTGTAATGAAAGCCCATGGAGCTCATCTGAAAGCTAGTGCTGCA ATGGTCCGTTTAAGACTTTATGATATCTTGGCTTTGTTACCTCCAAAAACTTATGAAGGATCTTTTAAT GCACTTCTTAGAGAACTGGTAGCGGAATTCACTTTGACTGACAACTCAGCCAACACAACTACTTCCCTC CTCAGATCCCTCTGCCATTATGATGATAGTGTTCTTCTTGGTTCTTGGCTTCAGGAAACTGATCATAAA TCAATTGAAGACCAGCTCCAGCCAAACAGTGCCTCTGGAAGTGGGGCTCTGGAGCATGATCCTTCCTCT ATTTATTTACGCATACCTGCTGGAGAAGCAGTACCTGGTCCCCTCCCTCTCGGAGTCTCAGTCATTGAT GCTTCTGTGGCCCTTTTTGGTGTAGTGTTTCCTCATGTTTCTTATAAACACCGATTACAAATGTTGGAT CACTTTGCTGAATGTGTTAAACAAGCTAAAGGTGTCCGCCAGCAGGCTGTGCAGCTTAACATATTTACT GCTGTTCTTAGTGCACTAAAGGGCTTAGCTGAAAACAAAAGTACTTTAGGACCTGAGGAAGTTCGTAAA TCTGCCCTGACACTGGTTATGGGTCCTCTGGACAACCCAAACCCCATCTTACGTTGTGCAGCGGGGGAA GCTCTTGGAAGAATGGCTCAGGTGGTTGGAGAAGCAACTTTTATTGCTAGAATGGCCCAATATAGCTTT GACAAGTTGAAATCGGCTCGAGATGTTGTATCTAGGACTGGTCATTCATTGGCTCTTGGTTGTTTGCAT CGTTATGTTGGTGGAATAGGCTCAGGACAACATCTGAAGACCAGTGTCAGCATTTTATTGGCTCTAGCA CAAGATGGGACATCCCCTGAAGTCCAGACTTGGTCTCTTCATTCACTTGCTTTGATAGTGGATTCTAGT GGTCCGATGTATCGTGGCTATGTGGAACCAACATTATCTCTAGTTCTTACCTTGCTGTTGACAGTTCCG CCTTCACATACAGAAGTTCATCAGTGTTTGGGTCGATGCTTGGGTGCTATAATAACTACTGTTGGCCCT GAACTACAAGGGAATGGAGCAACAACTTCTACAATTCGTTCCTCTTGTTTGGTGGGTTGTGCAATAACA CAAGACCATTCAGATTCTCTTGTTCAGGCAGCTGCCATATCTTGCCTTCAGCAGCTTCACATGTTTGCA CCACGACATGTCAATCTATCTAGCCTTGTTCCTAGCCTTTGTGTTCACTTATGTAGTTCCCATTTGTTA CTTCGACGAGCAGCTGTGGCATGTCTTCGGCAACTTGCACAAAGAGAAGCAGCGGAAGTATGTGAATAT GCCATGAGCCTGGCAAAAAATACAGGGGACAAAGAGAGCAGTAGTGCCAATGTCAGTCCTTTTGCCCCT GGGGTTAGTTCTCGAACTGATATCCATTGCCGGCACCAAGGTGTTAATATAACAGAAACTGGTCTTGAG GGACTTCTTTTTGGAATGCTAGACCGGGAGACAGATCGAAAATTATGTTCTGATATTCATGACACTTTG GGACATATGCTTTCTTCGCTGGCAGTAGAAAAACTTTCTCATTGGCTAATGCTTTGTAAAGATGTCCTG GCAGCTTCTAGTGATATGAGTACTGCAACTCTCTTAAGTAGTGGAAAAGATGAAGAAGCTGAAAAGAAA GATGAGATGGATGATGATACCATGTTTACCACGTTAGGTGAAGAAGATAAATCAAAGCCCTTTGTGGCC CCTCGCTGGGCCACTCGAGTATTTGCTGCCGATTGCCTGTGTCGAATCATCAATTTGTGTGAGAATGCA GACCAGGCTCACTTTGATCTTGCCTTGGCACGTTCTGCTAAACTTCGAAACCCTACAAATGACCTCTTG GTACTTCATCTCTCTGACCTCATTCGCATGGCATTCATGGCTGCAACTGATCATAGCAACCAGCTGCGA ATGGCTGGGCTCCAGGCGCTTGAAGACATTATCAAGAAGTTTGCGTCTGTGCCTGAGCCAGAATTTCCA GGTCATGTGATACTGGAGCAGTATCAGGCTAATGTGGGAGCTGCTCTAAGACCAGCCTTTTCACAAGAT ACACCATCAGATATAATAGCGAAAGCTTGCCAGGTATGTAGTACATGGATAGGAAGTGGAGTTGTCAGT GATCTCAATGATCTCCGTCGAGTACACAATCTTCTTGTTTCTTCTCTGGACAAAGTTCAGGCTGGAAAA GGATCTTCCAGCCAGCTGTACCGAGAGAGTGCCACGACCATGGAAAAACTGGCTGTTCTCAAAGCTTGG GCAGAGGTATATGTGGTCGCTATGAATATTAAAAAGGAAGCAGAGTCAAAACCAAAAAGAGCAATTAAA AATACTGACGATGATGATGACGACTGTGGTACCATCGATGAACTGCCACCAGATAGTTTAATAACACTG GTACAACCTGAACTACCAACACTCAGTCGCCTGTGGTTAGCAGCATTAAAAGATTATGCACTCTTGACT TTACCAGCCGAATTTTCTAGTCAGCTTCCTCCAGATGGTGGAGCATTTTATACCCCTGAAACTATTGAT ACAGCTAGACTTCACTATCGGAATTCCTGGGCCCCAATTCTCCATGCGGTGGCACTTTGGTTAAATAGC ACAGGATTTACGTGCTCAGAGTCTACAGAAGCAGCAGCAATATCTGGTTTACAAAAACGTTCTACATCT GTCAATTTAAACCAGGCATCAGGAGCAGTGGGTAGTGCTAAATCTTTGCCAGAAATTAACAAAGACAGA ATGCATCTGATTTTAGGTGTGAGTATACAGTTTCTTTGTTCCCCTAGACCTGAGGAGCCCATTGAACAT GTTACAGCATGCCTGCAGGCCTTACATACCTTGCTAGACTCCCCTTATGCTCGAGTCCATATTGCAGAA GATCAGCTGATAGGTGTTGAGTTGCTGAGTGTTTTGCACCGCCTTCTATTGACCTGGAATCCATCATCT GTCCAGCTGTTGGTTACTGGAGTTGTACAACAGATAGTAAGAGCTGCTCAGGATTATTTGCAAGAGAAA AGAAACACTCTAAATGAAGATGATATGGAAAAAGAGGCCTGTACCGTATTGGGAGAAGGAGGTGACAGC GGTGGTCTCATTCCTGGAAAATCTCTTGTGTTTGCAACTATGGAGCTGCTGATGTTCATTTTAGTACGG CATATGCCACATCTCAGTACCAAGGTGTCAGACTCTCCAAGTCACATAGCCACTAAAACTCGACTATCA GAAGAAAGTGCTCGTTTGGTGGCAGCCACAGTTACCATACTCTCTGATTTACCATCCCTTTGTTCACCC GCTGGATGTATGACAATCCTGCCCACAATTCTGTTCTTAATTGCAAGAATATTGAAAGACACAGCAATA AAGTCTGCAGATAATCAGGTTCCTCCACCAGTCAGTGCAGCTCTTCAAGGGATTAAAAGTATTGTGACA CTTTCAATGGCCAAAACTGAGGCTGGCGTTCAAAAACAGTGGACAGCTCTGATTCGTAGCACGCTTGCT TGCATCCTGGAATATTCTCAACCAGAAGACTCTGTACCTACACCTGATGAAGTAAGCATGCTAACAGCA ATTGCACTCTTCCTGTGGTCTGCTAGTAATGAAATAATAGGAGTCCAGTCATTACAGAATGGCTGCATG AACAGATTTAAAAATGCATTAAATTCATGCGACCCATGGGTTCAAGCCAAATGTTACCAGCTTCTCCTC TCAGTCTTCCAGCATTCCAATCGTGCCCTTTCAACTCCTTATATTCATTCATTAGCTCCAATAGTGGTT GAAAAGCTAAAAGCTGTTGAAAGAAACAGACCAGCCAGTAACATAGAGCTTTTAGCGGTTCAAGAAGGA ATAAAAGTTCTTGAAACACTGGTTGCTCTTGGTGAAGAACAAAACAGAGTCCAGCTACTTGCTCTTTTA GTTCCCACTTTGATATCTTACCTGCTGGATGAAAATTCTTTTGCCTCAGCAAGTTCAGCTTCCAAAGAT CTTCATGAGTTTGCACTCCAGAATTTAATGCATATTGGACCTCTGTATCCACATGCTTTCAAGACAGTA ATGGGGGCTGCTCCTGAGTTGAAAGTGCGTCTAGAAACTGCCGTTCGAGCAAGCCAAGCGAGCAAAGCT AAAGCGGCTGCCAGACAACCAGCCCCCGCCATACATTCTGCACCAACAATTAAGCTAAAAACAAGTTTT TTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_019024
Insert Size	6216 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_019024.2
RefSeq Size	6820 bp
RefSeq ORF	6216 bp
Locus ID	54497
UniProt ID	Q9P2D3
MW	224.3 kDa
Gene Summary	Component of clathrin-coated vesicles (PubMed:15758025). Component of the aftiphilin/p200/gamma-synergin complex, which plays roles in AP1G1/AP-1-mediated protein trafficking including the trafficking of transferrin from early to recycling endosomes, and the membrane trafficking of furin and the lysosomal enzyme cathepsin D between the trans-Golgi network (TGN) and endosomes (PubMed:15758025).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.