MRPL2 (NM_015950) Human Untagged Clone
CAT＃: SC304370
MRPL2 (untagged)-Human mitochondrial ribosomal protein L2 (MRPL2), nuclear gene encoding mitochondrial protein
CNY 6270.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CGI-22; MRP-L14; RPML14 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC304370 representing NM_015950.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCCTGTGCGCACTGACCCGCGCTCTGCGCTCTCTGAACCTGGCGCCCCCGACCGTCGCCGCCCCT GCCCCGAGTCTGTTCCCCGCCGCCCAGATGATGAACAATGGCCTCCTCCAACAGCCCTCTGCCTTGATG TTGCTCCCCTGCCGCCCAGTTCTTACTTCTGTGGCCCTTAATGCCAACTTTGTGTCCTGGAAGAGTCGT ACCAAGTACACCATTACACCAGTGAAGATGAGGAAGTCTGGGGGCCGAGACCACACAGGCCGAATCCGG GTGCATGGTATTGGCGGGGGCCACAAGCAACGTTATCGAATGATTGACTTTCTGCGTTTCCGGCCTGAG GAGACCAAGTCAGGACCCTTTGAGGAGAAGGTTATCCAAGTCCGCTATGATCCCTGTAGGTCAGCAGAC ATAGCTCTGGTTGCTGGGGGCAGCCGGAAACGCTGGATCATCGCCACAGAAAACATGCAGGCTGGAGAT ACAATCTTGAACTCTAACCACATAGGCCGAATGGCAGTTGCTGCTCGGGAAGGGGATGCGCATCCTCTT GGGGCTCTGCCTGTGGGGACCCTCATCAACAACGTGGAAAGTGAGCCAGGCCGGGGTGCCCAATATATC CGAGCTGCAGGGACGTGTGGTGTGCTACTGCGGAAGGTGAATGGCACAGCCATTATCCAGCTGCCCTCT AAGAGGCAGATGCAGGTGCTGGAAACGTGCGTAGCAACAGTAGGCCGAGTATCCAACGTTGATCATAAC AAACGGGTCATTGGCAAGGCAGGTCGCAACCGCTGGCTGGGCAAGAGGCCTAACAGTGGGCGGTGGCAC CGCAAGGGGGGCTGGGCTGGCCGAAAGATTCGGCCACTACCCCCCATGAAGAGTTACGTGAAGCTGCCT TCTGCTTCTGCCCAAAGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_015950 |
| Insert Size | 918 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015950.4 |
| RefSeq Size | 1333 bp |
| RefSeq ORF | 918 bp |
| Locus ID | 51069 |
| UniProt ID | Q5T653 |
| MW | 33.3 kDa |
| Gene Summary | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the EcoL2 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 12q. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205836 | MRPL2 (Myc-DDK-tagged)-Human mitochondrial ribosomal protein L2 (MRPL2), nuclear gene encoding mitochondrial protein |
CNY 2400.00 |
|
| RC205836L3 | Lenti ORF clone of Human mitochondrial ribosomal protein L2 (MRPL2), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC205836L4 | Lenti ORF clone of Human mitochondrial ribosomal protein L2 (MRPL2), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5890.00 |
|
| RG205836 | MRPL2 (tGFP-tagged) - Human mitochondrial ribosomal protein L2 (MRPL2), nuclear gene encoding mitochondrial protein |
CNY 4370.00 |
|
| SC320944 | MRPL2 (untagged)-Human mitochondrial ribosomal protein L2 (MRPL2), nuclear gene encoding mitochondrial protein |
CNY 2400.00 |
