C2CD3 (NM_015531) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	OFD14
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC304325 representing NM_015531. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAAACAACGAAAAGGCCAAGGGTCTGGGGGCAGCCGTGGGCGCAAAAAAAGAGGTTTAAGTGACATT TCTCCATCTACAAGCCTGCCACCTCTGGTTGAAGGCCAGCTACGCTGTTTTCTAAAACTTACTGTTAAT AGAGTCATATGGAAGATTGCAAAGCCTCCCACTTGTGTACTTGTCCGAGTGAGATGGTGGGGAGAAACA TCAGATGGAACCCTCTTTTGTCCCAGGGATGCATTGCAGACTGAACCAAAAGCTGTGAGAACAACTACA CGTTACGCTATTCGTTGTGGTCCAAAACAGTTTACCTCTTATCTAACAGATATGGCTGTGCTGGTGCTG GAAGTAATCACCAAACTTGATGGTCTTCCAATTGGTAGAGTTCAGATCAATGGACTAGCTCAACTTTCT CCAACCCATCAAATCAATGGATTTTTTACCATTGTTTCATCAACGTCTAAGAAACTTGGAGAACTCCAG GTCTCACTTGCCCTGGAACCTCTGTCAGAAACTTACGACAGCTACCATCCACTTCCTACCACTGACATG ACAGAAAATGTGCTTTTATCTAAGCAGGGATTCAGAGAGAATACTGAACCCAGCAGTACCCAGTTTCAG GTTCCATCAAGGCCTCGCGACATACATACCATCAAAATTGATGGAAAAGAGTTAGCAGCCAACAGCAGT AGATCAACCACTCCGAGGGGAAAAGACCATGTATGCTTTGCAGAGAACCCTGATACAATAAAGGATTCT TCCTTTGGACTACAGCACAGTCTTAATTCAGGACAGAGTTTAGAGTCTGTAACTCTGAAAGGCAGAGCT CCACGGAAGCAGATGTCCCTTCTGAACAGTTCTGAATTCCAGCCTCAAATTAGAACAGTTGCCAAGAGT CACAGTGACTCATGCATTCTTTCTTCAAACAACCTCCCTACCAAGGATCTTCTTTCAGCTCTGTTAGAA CAAGGCAATAAACTGCGTAATGCCATGGTGATTTCTGCAATGAAATCAAGCCCAGAGACCAGCATGTTG TTGGACCAAGTTCATCCTCCTATTAATGAAGATTCTCTTAGAGCATCAACACAGATCAGAGCCTTTTCT AGGAATCGGTTTAAAGACCACATTGAAGATCACCTCCTCCCTTCAACTGAGAATACATTTTGGAGACAT GACACAAAAGCTGATACCAGAGCTATACAGCTGCTATTAGGCAGTGCTGAATTATCCCAAGGCAATTTC TGGGATGGGCTAGGCTCTCCTCCAGATTCCCCATCTCCTGGGAGTGATGTGTATTGCATCAGTGAGCTG AATGACCCTCAGTATGACCAGAGTCTTCTGGAGAATTTATTTTATACAGCACCTAAATCTGATACCAGC ATCAGTGATTTCCTCAGTGAAGAGGATGATATCGTCCCTTCTAAAAAAATAAGCCAGTCAACAGCTCTT GCCAGATCATCTAAGGTTCTGGAGTCAAGTGATCATAAGCTGAAGAAGAGGTCAGCAGGCAAGAGAAAT AGAAATTTGGTTGAACAACAGATGCTCTCAGAAACTCCAGAAGATGCCCAAACGATGACACTAAGTGTG GATAGACTGGCCCTTTTGGGTAGAACACATTCAGTCAGAATCATCATCGAAACCATGGGAGTTCCTCCA GATAGTCCTCAGATGACCCCTGGCAAAAAAAGCTATGCTGGTCCACCACCTAAGGTGACTACAGCAAAA AAGCGCACTTTCTTTGTAGAATATCACTTTCCTGTGGGCTTTTCGGAAAGCGGATTGGGAAAGACAGCT TTGATCACTGAGGTTGTTCGACTCGCCTCCAGTAAAATTACAGATGGAAAGGTGAAGTTCCAGCAGCGA TTTGTGTTTCCAGTACAGTTTGGTGGCCCAATGATAGAGCACTGGTGGAATTCCAACCTCACTTTCCAA ATTTATGTAAAGAAAACTCCACAGAAAAAGCCAGAAGTCATTGGATCTGTGTCACTTTCTTTGCGAGCT GTCATTCAATCAGAGCTGCTTTCTTTCAGTGATCAGCTTCCAGTGCAACAAGAAAATGGTCAGTCTCCA TTTGGCCCCCTCAAGGTAACCATGGAGCTTATTACAGATAACAAAGATTTCACTGGTATCAATACTAAG TTAAGTGGCAACACCCATTATACCCCACTTTGTGCTCCTACAAGTCCAAATAAGGCACTACCAGAACTT AACCAAGATATGACCTGTACCAAAAATCCACAAAACTTAAACCAAATTCATGAGGAAACTGCAAAGAAA GCACAGAACTTGGTGCTCCCCAACCGAAAGTCACCAAGCCCTGTAGCACCACATCCTTCAACCTTCGTA GCTACGCCAGCCTCCCATAATTTAGTCAATCAGACAAATGGGACAACAAAAGAGAGTGCTTTGCTGTTG CATGTGCTGTTGATGGTGCCAGATGGGAAAGATTTTATTAGTGGAGAATCTGAGAAACAATCACCATGC AATGTTTATTTAAATTGTAAACTCTTCAGCACAGAGGAAGTCACCAGATCTGTCATCGCATGGGGCACA ACACAACCGGTCTTTAACTTTTCTCAGGTGATTCCTGTCTCTCTGTCTTCCAAATACCTGGAAAGGCTT AAGAACAATGTGATGGTAATTGAAACTTGGAATAAGGTGCGGAGCCCAGGACAGGACAAGCTGCTCGGG CTGGTGAAACTTCCCCTCCACCAGTTTTACATGTCATTCAAAGATGCTAAGATTTCTCGCCTGCTGCTG GATGCCCAGTACCCAGTTGTTGCTGTCGACAGCTACATGCCTGTGATTGATGTGTTTTCAGGCCACCAA AATGGGAGTCTTCGAGTCTTTTTAGCTATGGGTTCTTCAAATCAAATAATGGCACTACAAAGATTAAAG AATGAAGAAGGAACACTCCCTCCCTTCAGCCCTAGGCCAGCCCATTTCCTGGACCAGCCAACTGCAGCA TCTGTTGCTATGGCAGAGGACCGAGGAAATGGACTGATGGAGCACTGCTTTGAGATCCATATAGAGATG GTTAAAGGGCTAGCCCCTCTTCAGGCAACAGTCTGGGGAGAAGCAGATTGTTATGTCCAGTACTACTTT CCAGTTCAACACTCTCAATCCAGTGTGCTGAAAGGACCTGAGTTCCTTGAAAATGGAATTACTCTGAAG CCCTTCAGAACTGCAACCACACTCTGTGTTCCAGATCCCATCTTTAATAGTGAACACCATCACTCTCTC CTGTTGCCAGCTGAGGTTCCAGTGCAAAGGCTCCTACTAAGTGCTTTCTCTGCACAGGGCCTCGTGCCT GGAGGTGGAGTCCAGTTTGAAATCTGGTGCAGATACTATTATCCTAATGTGAGAGACCAGAAGGTCGCC AAAGGAACCTTGCCATTATCAAGGATCTGTGCTATGGTAACCACCCAGCATCGTGAGGATGTGGGAATA CAGACCTTTAATCTCCCTTTAACCCCCAGGATTGAGAACAGGAAAGAATTGAGGAACCAGTCATCAGGT TTACTGGATGTGGGCCTAAGGTACAGGCGTAGTCCAAGAACAGCAGAGGGAGTTCTTGCTGCCCGAACT GTTTCCATCTCAGTCCAGATTATCAGAGCCTGTGGTCTGCAAGCAGCAGCCAAGGCTTTGGCTGAACGG GAACCCGCTCTACAGTTTAGTGCCACAGTCGGGGTCAATGCCTCTGTCACCACTCATCTCTCCTTCCTG CCCCAGGGAGAACAGCGCCGAACCCACCCTGTGGCCTGTTCTTTCTGCCCTGAGTTCTCCCATCACGTT GAGTTCACATGTAACTTGGTGACTCAGCACTGTAGTGGAGAGGCCTGTTTCCTAGCAGAGTTGTTGGAG TTTGCAGAAGTTATTTTTGCTGTCTATCATGAAAATACCAAGTCAGCAAGTGATATAATCAGTATTGAG TCATGCAAAGAGTATCTGCTTGGAGTAGTAAAAGTTCCAACAAAAGAGCTGCTGATCAAGAGATCTGGG ATCACAGGATGGTATCCTATCATTTTACCAGAAGACGGGGGCCTACCTCATGGCCTGGAGCTCATGCAG AAGATCGTGGGTGGTCTGGAGCTTTCGATTTCCTTCACGCATCGTGGAGATAGAGAACGGGTGTTGGAA GCTGCTGAGCATTTGGGCTGGAGCTTTGAGAACAGCCTGAAAGATTTTGTCAGAATGGATGAAGGGGAG CCAGCCACTGTCACCATCTCCACCCCAAGGCTGTGGCTGCCCATCCATTGTGTGCTGCTTGCTGGCCAC AACCACATTCATAAGAATACATATTGCTACCTTCGCTACAAGTTCTATGATCATGAAGCCTTTTGGACC CCTCTCAAGAAGCCTAAGGAATCTGTAAACAAAAAGCAGATTATGGTCACTTTCAAGGCATCCAAAAGA GCAGAAGTCACCAGGGGCCCATCACTGCTTTGGTACTTCAGGGAGGAGAGGCTAGAGATCCAAGTGTGG CGAGCTTATGGCAATGACAGTGTGGAGAGACCCCATCAGACAGACAGCTGGATTGGCTCAGCCTATGTG GACCTGGCCAGACTTGGGGAGAGGTCAGCGAGGACGCTAACTGTCAGTGGTGTGTATCCTCTGTTTGGA CGAAATGCTTCCAACCTCTCAGGAGCTGCCTTGCGAGTTCATGTGGTTCTTTCCTCTCTTTCCTCACAC CTTGAGCCCACTCATGAGCTGGACTCCATGGACTGCAGCAGCCACAGTGAGTCTGAGCAGCTCCCCAGA AGGAATGATGAGGTCCAGCTCTCTCCACCAGAAGTCATCTCCTGCCACCAGAAGTCTCCTGCCTCCACC CAGGTCCCCTGCAGCAGCACCACAGCTGAAGTCCGCCTGACGCAGGAGGGCCCTGCTGATTTGGATGGA ACGTTTGCAGTCAGCATCCTAGTAGAAAGAGCAATGCACTTGAGCTTGAAAGGGAGCCCCTTGACAGAG CGGAAAGTATCGATACCCAGTTGTTGTGTATCCTTTGCAACAGCCGATGAGTCATCTCCTGTATACACC CAAGTGGTTGAAAACACAGATTCCCCCATCTGGAATTTTCAACAGCAGTCAAGGCTATCAAAAGAGCTG CTTCTGGACCCACAACAAACCCTGGTCTTCAAAGTTTGGCATAAAGGAGATGAGGAGAGGGTGATTGGC TTTGCCTCGGTGGACCTCTCCCCACTTCTCTCTGGCTTCCAGTTTGTCTGTGGCTGGTACAACATCACA GACTTCAGTGGAGAGTGCCAGGGGCAGATAAAAGTTGCTGTCTCCCCTTTGGAGAGTTTGATACACTTC AAAGAAGAAAGGCAAGCAAGGCGTGGAGTGGAGACCTCAAAATCACTGATCCCAATATACAGTCCCTTT TCCTTCCCTGCCTCTGATACGTATGCTGCATTCTCCAGCCACATGGCAAGGCAGACCCTAGACCAACTT GCTCATGCCTCCTCAAAGGAGCTTGATTTCTCCTCTCCTGGGAGAAGTGATACCACAAGAAGCCAAGCA TCACGCCATGAAGAGCATGTGCAGAACATTCGCCGGTTTCATGAATCCCTGCATCTTCAGGGAGAGGCA CCCTTGCCATGTGATGACAAACTGACCACATCACCTTTGTCCTCCCAAACCTCCATTCTGACTTCTCTC AGGAAGAATCTGAGTGAGCTTGATCAGATTCAGAGGTACTTCCGCCAGAAGCTCACCAAGCCTTTCCTA CCCCTCAGCCCTCAGACTCAAACGGCCATCTCACAGCACCAGGAGAGCTGTAGGGACCATCTTGGGCCA GGTGCCAGCAGCCTAGACCCTGGGAGCCAGTGTATCCTGGAGAAATCCAGTAACCTGGTGTTGCAAGTC AGCTCCTTAATCACAGGTAGTTACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_015531
Insert Size	5892 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_015531.5
RefSeq Size	6285 bp
RefSeq ORF	5892 bp
Locus ID	26005
UniProt ID	Q4AC94
MW	218.1 kDa
Gene Summary	This gene encodes a protein that functions as a regulator of centriole elongation. Studies of the orthologous mouse protein show that it promotes centriolar distal appendage assembly and is also required for the recruitment of other ciliogenic proteins, including intraflagellar transport proteins. Mutations in this gene cause orofaciodigital syndrome XIV (OFD14), a ciliopathy resulting in malformations of the oral cavity, face and digits. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2014] Transcript Variant: This variant (2) lacks multiple 3' exons and contains an alternate 3' terminal exon, resulting in a distinct 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (2) has a distinct C-terminus and is shorter than isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.