KIAA0427 (CTIF) (NM_014772) Human Untagged Clone
CAT＃: SC304169
CTIF (untagged)-Human CBP80/20-dependent translation initiation factor (CTIF), transcript variant 1
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | Gm672; KIAA0427 |
| Vector | pCMV6 series |
| Sequence Data |
>NCBI ORF sequence for NM_014772, the custom clone sequence may differ by one or more nucleotides
ATGGAAAACTCCTCTGCAGCATCAGCCTCCTCGGAGGCAGGGAGCAGCCGCTCCCAGGAG ATCGAGGAGCTGGAGCGCTTCATCGACAGCTACGTGCTGGAGTACCAGGTGCAGGGGCTG CTGGCTGACAAGACGGAGGGTGATGGCGAGAGCGAGAGGACCCAGTCCCACATCTCCCAG TGGACAGCGGACTGCAGCGAACCGCTGGACAGCAGCTGTTCCTTCTCCCGAGGGCGAGCC CCCCCACAGCAGAATGGCAGCAAAGACAACTCTCTGGACATGCTGGGCACGGACATCTGG GCGGCCAACACCTTCGATTCCTTCAGTGGTGCCACCTGGGACCTGCAGCCGGAAAAGCTG GACTTCACCCAGTTCCACCGCAAAGTCCGACACACGCCCAAGCAGCCCCTGCCACACATC GACCGCGAAGGGTGTGGCAAAGGGAAGCTGGAAGATGGGGATGGCATCAACCTGAATGAC ATCGAGAAGGTCCTTCCAGCCTGGCAGGGCTACCACCCGATGCCCCATGAAGTGGAGATC GCACACACCAAGAAGCTGTTCCGCAGGAGGAGAAATGATCGAAGGCGGCAGCAGAGACCT CCGGGGGGCAACAAGCCCCAACAGCATGGTGACCACCAGCCAGGCAGTGCCAAACACAAC AGGGACCACCAGAAATCCTACCAGGGGGGCTCAGCACCCCACCCCTCAGGGAGGCCCACT CACCATGGCTACAGCCAGAACCGGCGCTGGCACCATGGCAACATGAAGCACCCACCAGGC GACAAGGGGGAGGCAGGCGCACACCGCAATGCCAAAGAGACCATGACCATCGAGAACCCA AAACTGGAGGACACTGCAGGGGACACCGGGCACAGCAGCCTTGAGGCCCCCCGCAGCCCT GACACCCTGGCCCCGGTGGCTTCTGAGCGGCTGCCCCCACAGCAGTCAGGGGGGCCAGAG GTTGAGACAAAACGTAAAGACAGTATTCTTCCCGAGCGCATCGGGGAGCGGCCCAAAATT ACCCTGCTCCAGTCTTCCAAAGACAGACTGCGGCGAAGGCTAAAGGAAAAGGATGAAGTG GCCGTGGAGACGACCACTCCCCAGCAGAACAAGATGGACAAGCTGATCGAGATCCTGAAC AGCATGCGGAACAACAGCAGCGACGTGGACACCAAGCTCACCACCTTCATGGAGGAGGCC CAGAACTCCACCAACTCCGAGGAGATGCTGGGCGAGATCGTGCGCACAATCTACCAGAAG GCTGTGTCCGACCGCAGCTTCGCCTTCACCGCTGCCAAGCTCTGCGACAAGATGGCGCTC TTTATGGTGGAGGGGACCAAGTTCCGGAGCCTGCTCCTCAACATGCTGCAGAAGGACTTC ACGGTGCGCGAGGAGCTGCAGCAGCAGGACGTGGAGCGCTGGCTGGGCTTCATCACCTTC CTGTGCGAGGTCTTCGGCACCATGCGCAGCAGCACAGGCGAGCCCTTCCGTGTGCTCGTG TGCCCCATCTACACCTGCCTCAGGGAGCTCTTGCAATCTCAGGATGTGAAGGAAGATGCT GTCCTTTGCTGCTCTATGGAGCTGCAGAGTACAGGCCGGCTGCTGGAGGAACAGCTGCCT GAGATGATGACAGAGCTCCTGGCCAGCGCACGGGACAAGATGCTGTGCCCCTCGGAGTCC ATGCTGACCCGGTCGCTGCTCCTAGAGGTCATCGAGCTCCACGCTAACAGCTGGAACCCT CTGACGCCCCCCATCACGCAGTACTACAACAGAACCATCCAGAAACTGACAGCCTGA |
| Restriction Sites | Please inquire |
| ACCN | NM_014772 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014772.1, NP_055587.1 |
| RefSeq Size | 5737 bp |
| RefSeq ORF | 1797 bp |
| Locus ID | 9811 |
| UniProt ID | O43310 |
| Gene Summary | CTIF is a component of the CBP80 (NCBP1; MIM 600469)/CBP20 (NCBP2; MIM 605133) translation initiation complex that binds cotranscriptionally to the cap end of nascent mRNA. The CBP80/CBP20 complex is involved in a simultaneous editing and translation step that recognizes premature termination codons (PTCs) in mRNAs and directs PTC-containing mRNAs toward nonsense-mediated decay (NMD). On mRNAs without PTCs, the CBP80/CBP20 complex is replaced with cytoplasmic mRNA cap-binding proteins, including EIF4G (MIM 600495), and steady-state translation of the mRNAs resumes in the cytoplasm (Kim et al., 2009 [PubMed 19648179]).[supplied by OMIM, Dec 2009] Transcript Variant: This variant (1) represents the more predominant transcript and encodes isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208324 | CTIF (Myc-DDK-tagged)-Human CBP80/20-dependent translation initiation factor (CTIF), transcript variant 1 |
CNY 5990.00 |
|
| RC208324L3 | Lenti-ORF clone of CTIF (Myc-DDK-tagged)-Human CBP80/20-dependent translation initiation factor (CTIF), transcript variant 1 |
CNY 7890.00 |
|
| RC208324L4 | Lenti-ORF clone of CTIF (mGFP-tagged)-Human CBP80/20-dependent translation initiation factor (CTIF), transcript variant 1 |
CNY 7890.00 |
|
| RG208324 | CTIF (tGFP-tagged) - Human CBP80/20-dependent translation initiation factor (CTIF), transcript variant 1 |
CNY 6560.00 |
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