NRAP (NM_006175) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	N-RAP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC303742 representing NM_006175. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAATGTGCAGCCCTGTTCTAGGTGTGGGTATGGGGTTTATCCTGCCGAGAAGATCAGCTGTATAGAT CAGATATGGCATAAAGCCTGTTTTCACTGTGAAGTTTGCAAGATGATGCTGTCTGTTAATAACTTTGTG AGTCACCAGAAAAAGCCGTACTGTCACGCCCATAACCCTAAGAACAACACTTTCACCAGTGTCTATCAC ACTCCATTAAATCTAAATGTGAGGACATTTCCAGAGGCCATCAGTGGGATCCATGACCAAGAAGATGGT GAACAGTGTAAATCAGTTTTTCACTGGGACATGAAATCCAAGGATAAGGAAGGTGCACCTAACAGGCAG CCACTGGCAAATGAGAGAGCCTATTGGACTGGATATGGGGAAGGGAATGCTTGGTGCCCAGGAGCTCTG CCAGACCCCGAAATTGTAAGGATGGTTGAGGCTCGAAAGTCTCTTGGTGAGGAATATACAGAAGACTAT GAGCAACCCAGGGGCAAGGGGAGCTTTCCAGCCATGATCACACCTGCTTATCAAAGGGCCAAGAAAGCC AACCAGCTGGCCAGCCAAGTGGAGTATAAGAGAGGGCATGATGAACGCATCTCCAGGTTCTCCACGGTG GTGGATACTCCTGAGCTGCTACGGAGCAAGGCTGGGGCACAGCTTCAAAGTGATGTGAGATACACAGAG GACTATGAACAACAAAGAGGGAAAGGCAGTTTCCCTGCGATGATCACACCCGCCTATCAGATAGCCAAA AGAGCCAATGAGCTGGCAAGTGATGTGAGGTACCATCAACAATATCAAAAAGAAATGAGGGGAATGGCT GGTCCAGCCATTGGAGCTGAGGGCATCTTGACAAGGGAATGTGCAGACCAATATGGCCAGGGTTACCCG GAGGAGTATGAGGAGCACAGGGGAAAGGGCAGCTTCCCAGCTATGATCACTCCAGCATATCAGAACGCC AAGAAAGCTCACGAACTCGCTAGTGACATAAAATACAGGCAGGACTTCAATAAGATGAAAGGCGCTGCA CATTATCACTCGCTTCCAGCTCAAGACAACTTGGTTCTCAAACAGGCTCAGAGCGTAAACAAACTCGTG AGTGAGAATAAATATAAAGAAAACTACCAGAACCACATGAGAGGCCGCTATGAAGGAGTTGGTATGGAC AGACGCACTCTGCATGCTATGAAAGTTGGCAGCCTGGCAAGCAACGTTGCCTACAAAGCTGATTATAAA CATGATATTGTCGACTACAACTACCCAGCCACTCTCACGCCTTCCTATCAAACAGCTATGAAACTGGTG CCCTTGAAAGATGCCAATTATAGGCAGAGCATCGACAAGTTGAAGTACAGCTCGGTGACTGACACCCCA CAGATTGTTCAAGCCAAAATCAATGCCCAGCAGCTGAGTCATGTGAATTACCGTGCTGACTATGAGAAA AATAAGTTGAATTACACATTGCCCCAGGATGTTCCTCAGCTGGTGAAGGCCAAAACCAATGCCAAACTC TTCAGTGAGGTTAAGTATAAAGAAGGCTGGGAGAAGACAAAGGGGAAAGGATTTGAGATGAAGCTGGAT GCCATGTCTCTGCTGGCCGCCAAAGCCTCTGGGGAGCTTGCTAGCAATATTAAATATAAAGAAGAATAT GAAAAGACAAAAGGCAAAGCCATGGGAACAGCCGACTCTAGGCTTCTGCACTCCCTGCAGATTGCTAAG ATGAGCAGTGAGGTTGAATATAAGAAAGGCTTTGAAGAGAGTAAGACCCGGTTTCACCTGCCCATGGAT ATGGTAAACATCAGGCATGCTAAGAAGGCCCAAACTCTCGCCAGTGACCTGGACTACAGGAAGAAACTG CATGAATACACTGTGCTGCCTGAAGATATGAAGACTCAGTGGGCCAAGAAGGCCTATGGGCTCCAGAGC GAGCTGCAGTACAAGGCTGACCTGGCATGGATGAAGGGAGTTGGGTGGCTGACAGAGGGGAGTCTCAAC TTGGAACAAGCCAAGAAGGCTGGACAGCTGGTCAGCGAGAAAAACTACCGGCAGAGGGTCGATGAGCTG AAGTTCACCAGCGTGACCGACAGCTCCCAGATGGAGCACGCCAAGAAGAGCCAGGAGCTACAGAGCGGG GTGGCCTACAAGGCAGGAAATGAGCAGTCTGTCCATCAGTATACCATCAGCAAAGACGAGCCTCTCTTC CTGCAGGCCCGAGCCAATGCTGCAAATCTCAGCGAGAAACTGTATAAGAGCAGCTGGGAAAACCAGAAA GCAAAAGGGTTTGAGCTGCGTCTTGATTCCTTGACCTTCCTGGCAGCCAAAGCCAAGAGGGACCTGGCT AGCGAGGTGAAGTACAAGGAGGATTATGAGAGATCCAGAGGGAAGCTCATTGGGGCAAAAGATGTACAG GGAGATTCGCAAATGAGCCACTCACTGCAAATGTCCAAGCTGCAGAGTGAGCTGGAGTACAAGAAGGGA TTCGAGGACACCAAATCCCAATGCCACGTCTCCCTGGACATGGTCCACCTCGTGCATGCCCGCAAAGCT CAGCATTTAGCCACAGACGTAGGCTACAAGACAGCGGAACATCACTTTACGGCTTTGCCCACAGACATG AAGGTGGAATGGGCCAAGAAGGCTTATGGCTTACAGAGTGATAACCAATACAGGGCAGATGTGAAGTGG ATGAAAGGCATGGGCTGGGTCGCCACCGGGTCATTAAATGTGGAGCAGGCGAAGAAGGCAGGAGAACTC ATTAGCGAGAAGAAGTACCGTCAGCATCCAGATGCTTTGAAGTTTACCAGTATTAAAGACACTCCGGAG ATGGTCCAGGCCAGAATTAGTTATACCCAAGCAGTGGATAGATTATACAGGGAACAAGGAGAAAACATA AAGCATCATTACACACCGACTGCTGACCTCCCTGAAGTCCTGCTGGCCAAGCTGAATGCCATGAATATC AGTGAGACGCGTTATAAGGAATCCTGGAGCAAACTTCGAGATGGTGGCTATAAACTGAGGTTGGATGCC CTTCCATTCCAAGCAGCAAAGGCTTCTGGTGAAATCATAAGTGATTACAAATACAAAGAGGCATTTGAG AAAATGAAAGGACAGATGCTTGGTTCCCGGAGCTTGGAAGATGATATCAGCCTTGCACATTCAGTGTAT GCGACCTCACTGCAGAGTGATGTGAATTACAAGAAAGGCTTTGAACACTCAAAGGCGCAGTTCCATCTG CCGTTGGACATGGCCGCCCTGGTGCATGCCAAGAAGGCTCAGACCCTGGCCAGCAATCAGGACTACAAA CATCCACTGCCCCAGTACACTTCCTTGGCAGAAGACCTGAGGCTGAGCTGTGCCAAGAAAGCTCACAAA TTGCAGAGTGAGAATTTGTACCGGTCAGACCTGAACTTTATGCGAGGTGTTGCATGTGTCATTCCAGGC ACGTTAGAGATTGAAGGGAGGAAGAAAGCATCGGAACTCATCAGTGAGAGTAAATATCGGCAGCATCCC CACTCATTCAAGTACACAGCTGTGACAGACACTCCCAACCTCCTTCATGCGAAATTCAGCAACCAGATA ACGAATGAGCGCCTCTATAAAGCAGCTGGAGAGGATGCAAGACACGAGTATACAATGACCCTGGGTCTG CCCGAGTTCATCCGAGCAAAAACGAATGCAGCCAACCTGAGTGACGCAAGATACAAAGAGTCCTGGCGT AATCTTCGTGCTCAAGGCTACAAGCTGACAATAGAAGCGCTCCCCTTCCAGGCTGCCCGGGCCTCTGGA GATATAGCCAGTGATTTTCTCTACAGACATGACTTTGTGAAGGAGCGAGGGAAACTCATAGGGCCCCAG AGTGTAAGAGACGACCCCCGGATCCAGCACTGCCGGCGCATGGGCCAGCTGCAGAGCGAGCTTCAGTAC AGGAGGGGGGCGACCAGCAGCCAAGCCCAGTTCCATCTGCCCATGGACATGGTGCACCTGGTCCATGCC AAGAATGCCCAGGCTCTGGCCAGCGACCACGACTACAGGACACAGTATCACAAGTTCACAGCACTGCCC GAGGACCTGAAGATGGCCTGGGCCAAGAAAGCCCATGCCCTGCAGAGTGAGTTGCGCTACAAGTCAGAC CTGATCGGCATGAAGGGCATAGGATGGCTGGCGCTGAGATCCCCACAGATGGAGAGTGCAAAGAAGGCT GGAGAACTCATCAGCGAGACCAAGTACCGTAAAAAACCAGACAGTATCAAGTTCACCACAGTGGTTGAC TCCCCAGACCTGGTTCATGCCAAGAACAGCTATATGCACTGCAATGAGCGCATGTATAGATCTGGAGAT GCAGAATCCCTGCACAGATACACCCTGATCCCCGACCATCCCGATTTCACCCGAGCTCGCCTCAATGCG CTGCATCTGAGTGACAAAGTCTACAGAAACTCCTGGGAGCAGACCCGGGCTGGCAGTTATGACTTCAGG CTGGATGCCATCCCCTTCCAGACTGCCCGGGCATCTAGGGAGATCGCCAGTGATTTCCGGTACAAAGAG GCTTTCCTGCGGGACCGAGGCCTGCAGATCGGGTACCGCAGTGTCGACGATGACCCAAGGATGAAGCAT TTCCTCAACGTTGGCAGGCTCCAGAGTGACAATGAGTACAAGAAGGACTTTGCCAAGAGTCGGTCCCAG TTTCACAGCAGCACAGACCAGCCCGGCCTCCTTCAGGCCAAGAGGAGCCAGCAGCTGGCCAGTGATGTG CACTACAGGCAGCCCCTGCCCCAGCCCACCTGCGACCCGGAGCAGCTGGGCCTCAGGCATGCTCAGAAG GCCCACCAGCTGCAGAGTGATGTCAAGTATAAATCAGACTTGAACCTGACCAGAGGTGTTGGCTGGACC CCTCCTGGCTCCTACAAAGTGGAAATGGCTCGGCGGGCTGCGGAACTGGCCAACGCAAGGGGCCTGGGT CTCCAGGGAGCTTACCGGGGGGCAGAAGCAGTGGAGGCTGGAGATCATCAGAGTGGGGAGGTGAACCCA GATGCCACTGAGATTCTGCACGTCAAAAAGAAGAAGGCCCTGCTGTTGTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_006175
Insert Size	5088 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_006175.4
RefSeq Size	5441 bp
RefSeq ORF	5088 bp
Locus ID	4892
UniProt ID	Q86VF7
MW	193 kDa
Gene Summary	May be involved in anchoring the terminal actin filaments in the myofibril to the membrane and in transmitting tension from the myofibrils to the extracellular matrix.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the shortest transcript and encodes the shortest isoform (1, also known as isoform C). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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