Otoferlin (OTOF) (NM_004802) Human Untagged Clone
CAT＃: SC303534
OTOF (untagged)-Human otoferlin (OTOF), transcript variant 2
CNY 23750.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AUNB1; DFNB6; DFNB9; FER1L2; NSRD9 |
| Vector | pCMV6 series |
| Sequence Data |
>NCBI ORF sequence for NM_004802, the custom clone sequence may differ by one or more nucleotides
ATGATCAAAACGGAGAAGTCCTACCCTGAGCGTCGCCTGCGGGGCGTCCTGGAGGAGCTG AGCTGTGGCTGCTGCCGCTTCCTCTCCCTCGCTGACAAGGACCAGGGCCACTCATCCCGC ACCAGGCTTGACCGGGAGCGCCTCAAGTCCTGCATGAGGGAGCTGGAAAACATGGGGCAG CAGGCCAGGATGCTGCGGGCCCAGGTGAAGCGGCACACGGTGCGGGACAAGCTGAGGCTG TGCCAGAACTTCCTGCAGAAGCTGCGCTTCCTGGCGGACGAGCCCCAGCACAGCATTCCC GACATCTTCATCTGGATGATGAGCAACAACAAGCGTGTCGCCTATGCCCGTGTGCCCTCC AAGGACCTGCTCTTCTCCATCGTGGAGGAGGAGACTGGCAAGGACTGCGCCAAGGTCAAG ACGCTCTTCCTTAAGCTGCCAGGGAAGCGGGGCTTCGGCTCGGCAGGCTGGACAGTGCAG GCCAAGGTGGAGCTGTACCTGTGGCTGGGCCTCAGCAAACAGCGCAAGGAGTTCCTGTGC GGCCTGCCCTGTGGCTTCCAGGAGGTCAAGGCAGCCCAGGGCCTGGGCCTGCATGCCTTC CCACCCGTCAGCCTGGTCTACACCAAGAAGCAGGCGTTCCAGCTCCGAGCGCACATGTAC CAGGCCCGCAGCCTCTTTGCCGCCGACAGCAGCGGACTCTCAGACCCCTTTGCCCGCGTC TTCTTCATCAATCAGAGTCAGTGCACAGAGGTGCTGAATGAGACCCTGTGTCCCACCTGG GACCAGATGCTGGTGTTCGACAACCTGGAGCTCTATGGTGAAGCTCATGAGCTGAGGGAC GATCCGCCCATCATTGTCATTGAAATCTATGACCAGGATTCCATGGGCAAAGCTGACTTC ATGGGCCGGACCTTCGCCAAACCCCTGGTGAAGATGGCAGACGAGGCGTACTGCCCACCC CGCTTCCCACCTCAGCTCGAGTACTACCAGATCTACCGTGGCAACGCCACAGCTGGAGAC CTGCTGGCGGCCTTCGAGCTGCTGCAGATTGGACCAGCAGGGAAGGCTGACCTGCCCCCC ATCAATGGCCCGGTGGACGTGGACCGAGGTCCCATCATGCCCGTGCCCATGGGCATCCGG CCCGTGCTCAGCAAGTACCGAGTGGAGGTGCTGTTCTGGGGCCTACGGGACCTAAAGCGG GTGAACCTGGCCCAGGTGGACCGGCCACGGGTGGACATCGAGTGTGCAGGGAAGGGGGTG CAGTCGTCCCTGATCCACAATTATAAGAAGAACCCCAACTTCAACACCCTCGTCAAGTGG TTTGAAGTGGACCTCCCAGAGAACGAGCTGCTGCACCCGCCCTTGAACATCCGTGTGGTG GACTGCCGGGCCTTCGGTCGCTACACACTGGTGGGCTCCCATGCCGTCAGCTCCCTGCGA CGCTTCATCTACCGGCCCCCAGACCGCTCGGCCCCCAGCTGGAACACCACGGGGGAGGTT GTGGTGACTATGGAGCCAGAGGTACCCATCAAGAAACTGGAGACCATGGTGAAGCTGGAC GCGACTTCTGAAGCTGTTGTCAAGGTGGATGTGGCTGAGGAGGAGAAGGAGAAGAAGAAG AAGAAGAAGGGCACTGCGGAGGAGCCAGAGGAGGAGGAGCCAGACGAGAGCATGCTGGAC TGGTGGTCCAAGTACTTTGCCTCCATTGACACCATGAAGGAGCAACTTCGACAACAAGAG CCCTCTGGAATTGACTTGGAGGAGAAGGAGGAAGTGGACAATACCGAGGGCCTGAAGGGG TCAATGAAGGGCAAGGAGAAGGCAAGGGCTGCCAAAGAGGAGAAGAAGAAGAAAACTCAG AGCTCTGGCTCTGGCCAGGGGTCCGAGGCCCCCGAGAAGAAGAAACCCAAGATTGATGAG CTTAAGGTATACCCCAAAGAGCTGGAGTCCGAGTTTGATAACTTTGAGGACTGGCTGCAC ACTTTCAACTTGCTTCGGGGCAAGACCGGGGATGATGAGGATGGCTCCACCGAGGAGGAG CGCATTGTGGGACGCTTCAAGGGCTCCCTCTGCGTGTACAAAGTGCCACTCCCAGAGGAC GTGTCCCGGGAAGCCGGCTACGACTCCACCTACGGCATGTTCCAGGGCATCCCGAGCAAT GACCCCATCAATGTGCTGGTCCGAGTCTATGTGGTCCGGGCCACGGACCTGCACCCTGCT GACATCAACGGCAAAGCTGACCCCTACATCGCCATCCGGCTAGGCAAGACTGACATCCGC GACAAGGAGAACTACATCTCCAAGCAGCTCAACCCTGTCTTTGGGAAGTCCTTTGACATC GAGGCCTCCTTCCCCATGGAATCCATGCTGACGGTGGCTGTGTATGACTGGGACCTGGTG GGCACTGATGACCTCATTGGGGAAACCAAGATCGACCTGGAGAACCGCTTCTACAGCAAG CACCGCGCCACCTGCGGCATCGCCCAGACCTACTCCACACATGGCTACAATATCTGGCGG GACCCCATGAAGCCCAGCCAGATCCTGACCCGCCTCTGCAAAGACGGCAAAGTGGACGGC CCCCACTTTGGGCCCCCTGGGAGAGTGAAGGTGGCCAACCGCGTCTTCACTGGGCCCTCT GAGATTGAGGACGAGAACGGTCAGAGGAAGCCCACAGACGAGCATGTGGCGCTGTTGGCC CTGAGGCACTGGGAGGACATCCCCCGCGCAGGCTGCCGCCTGGTGCCAGAGCATGTGGAG ACGAGGCCGCTGCTCAACCCCGACAAGCCGGGCATCGAGCAGGGCCGCCTGGAGCTGTGG GTGGACATGTTCCCCATGGACATGCCAGCCCCTGGGACGCCTCTGGACATCTCACCTCGG AAGCCCAAGAAGTACGAGCTGCGGGTCATCATCTGGAACACAGATGAGGTGGTCTTGGAG GACGACGACTTCTTCACAGGGGAGAAGTCCAGTGACATCTTCGTGAGGGGGTGGCTGAAG GGCCAGCAGGAGGACAAGCAGGACACAGACGTCCACTACCACTCCCTCACTGGCGAGGGC AACTTCAACTGGCGCTACCTGTTCCCCTTCGACTACCTGGCGGCGGAGGAGAAGATCGTC ATCTCCAAGAAGGAGTCCATGTTCTCCTGGGACGAGACCGAGTACAAGATCCCCGCGCGG CTCACCCTGCAGATCTGGGATGCGGACCACTTCTCCGCTGACGACTTCCTGGGGGCCATC GAGCTGGACCTGAACCGGTTCCCGCGGGGCGCAAAGACAGCCAAGCAGTGCACCATGGAG ATGGCCACCGGGGAGGTGGACGTGCCCCTCGTGTCCATCTTCAAGCAAAAGCGCGTCAAA GGCTGGTGGCCCCTCCTGGCCCGCAATGAGAACGATGAGTTTGAGCTCACGGGCAAGGTG GAGGCTGAGCTGCATTTACTGACAGCAGAGGAGGCAGAGAAGAACCCAGTGGGCCTGGCC CGCAATGAACCTGACCCCCTAGAGAAACCCAACCGGCCCGACACGAGCTTCATCTGGTTC CTGAACCCTCTCAAGTCGGCTCGCTACTTCTTGTGGCACACGTATCGCTGGCTGCTCCTC AAACTGTTGCTGCTCCTGCTGCTGCTCCTCCTCCTCGCCCTGTTCCTCTACTCTGTGCCT GGCTACCTGGTCAAGAAAATCCTCGGGGCCTGA |
| Restriction Sites | Please inquire |
| ACCN | NM_004802 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004802.2, NP_004793.2 |
| RefSeq Size | 4970 bp |
| RefSeq ORF | 3693 bp |
| Locus ID | 9381 |
| UniProt ID | Q9HC10 |
| Protein Families | Druggable Genome, Transmembrane |
| Gene Summary | Mutations in this gene are a cause of neurosensory nonsyndromic recessive deafness, DFNB9. The short form of the encoded protein has 3 C2 domains, a single carboxy-terminal transmembrane domain found also in the C. elegans spermatogenesis factor FER-1 and human dysferlin, while the long form has 6 C2 domains. The homology suggests that this protein may be involved in vesicle membrane fusion. Several transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) differs in the 5' UTR and coding region compared to variant 1. The resulting isoform (b, also called 'short form 1') has a shorter N-terminus and lacks a segment compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220950 | OTOF (Myc-DDK-tagged)-Human otoferlin (OTOF), transcript variant 2 |
CNY 9424.00 |
|
| RC220950L3 | Lenti-ORF clone of OTOF (Myc-DDK-tagged)-Human otoferlin (OTOF), transcript variant 2 |
CNY 14160.00 |
|
| RC220950L4 | Lenti-ORF clone of OTOF (mGFP-tagged)-Human otoferlin (OTOF), transcript variant 2 |
CNY 14160.00 |
|
| RG220950 | OTOF (tGFP-tagged) - Human otoferlin (OTOF), transcript variant 2 |
CNY 13490.00 |
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