MTRF1 (NM_004294) Human Untagged Clone
CAT＃: SC303463
MTRF1 (untagged)-Human mitochondrial translational release factor 1 (MTRF1), nuclear gene encoding mitochondrial protein
CNY 7220.00
Product images
CNY 1999.00
CNY 2700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MRF1; MTTRF1; RF1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC303463 representing NM_004294.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAATCGTCACCTGTGTGTTTGGCTTTTTAGACATCCATCTCTTAATGGTTACCTCCAGTGTCACATC CAGCTCCATTCTCATCAATTTAGACAGATACATCTTGATACAAGGCTGCAAGTTTTTAGACAAAACAGG AATTGCATTCTTCATCTGTTAAGTAAGAATTGGTCCAGGAGATATTGCCATCAAGACACCAAGATGCTC TGGAAGCATAAAGCACTACAGAAATATATGGAGAACCTGAGTAAGGAGTACCAAACACTTGAGCAATGT CTGCAGCATATCCCTGTGAATGAGGAAAACCGAAGGTCCTTGAACAGAAGGCATGCTGAGTTGGCACCT CTTGCAGCCATTTACCAAGAAATTCAGGAGACTGAACAAGCAATTGAAGAATTAGAATCAATGTGTAAA AGCCTAAATAAACAAGATGAAAAGCAGTTACAAGAACTTGCACTGGAAGAAAGGCAAACCATTGATCAA AAAATCAACATGTTGTACAATGAGCTTTTCCAGAGCCTTGTGCCAAAGGAGAAATATGACAAAAATGAT GTTATTTTAGAGGTGACAGCTGGAAGGACTACTGGAGGTGACATCTGCCAACAATTTACCCGAGAAATA TTTGACATGTACCAGAATTATTCGTGCTATAAACACTGGCAATTTGAACTTCTGAATTATACACCAGCA GATTATGGTGGACTACATCATGCAGCCGCCCGAATTTCCGGTGACGGTGTCTATAAGCATTTGAAGTAT GAGGGTGGGATTCACCGAGTTCAGCGCATCCCCGAGGTGGGCCTGTCCTCAAGGATGCAGCGCATTCAC ACAGGAACGATGTCGGTTATTGTCCTTCCTCAGCCAGATGAGGTGGATGTGAAATTGGACCCCAAGGAT TTGCGAATAGATACATTTCGAGCCAAAGGAGCAGGAGGGCAGCATGTTAATAAAACTGATAGTGCCGTC AGACTTGTCCACATCCCCACAGGGCTAGTAGTAGAATGCCAACAAGAAAGATCACAGATAAAAAATAAA GAAATAGCCTTTCGTGTGTTGAGAGCTAGACTCTACCAGCAGATTATTGAGAAAGACAAGCGTCAGCAA CAAAGTGCTAGAAAACTGCAGGTGGGAACAAGAGCCCAGTCAGAGCGAATTCGGACATATAATTTCACC CAGGATAGAGTCAGTGACCACAGGATAGCATATGAAGTTCGTGATATTAAGGAATTTTTATGTGGTGGG AAGGGCCTGGATCAGCTAATTCAGAGACTGCTTCAATCAGCAGATGAAGAAGCCATTGCTGAACTTTTG GATGAACACCTTAAATCAGCAAAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_004294 |
| Insert Size | 1338 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004294.2 |
| RefSeq Size | 2174 bp |
| RefSeq ORF | 1338 bp |
| Locus ID | 9617 |
| UniProt ID | O75570 |
| MW | 52.3 kDa |
| Gene Summary | The protein encoded by this gene was determined by in silico methods to be a mitochondrial protein with similarity to the peptide chain release factors (RFs) discovered in bacteria and yeast. The peptide chain release factors direct the termination of translation in response to the peptide chain termination codons. Initially thought to have a role in the termination of mitochondria protein synthesis, a recent publication found no mitochondrial translation release functionality. Multiple alternatively spliced transcript variants have been suggested by mRNA and EST data; however, their full-length natures are not clear. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (5) differs in the 5' UTR compared to variant 1. All four variants encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207912 | MTRF1 (Myc-DDK-tagged)-Human mitochondrial translational release factor 1 (MTRF1), nuclear gene encoding mitochondrial protein |
CNY 3656.00 |
|
| RC207912L3 | Lenti ORF clone of Human mitochondrial translational release factor 1 (MTRF1), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC207912L4 | Lenti ORF clone of Human mitochondrial translational release factor 1 (MTRF1), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5890.00 |
|
| RG207912 | MTRF1 (tGFP-tagged) - Human mitochondrial translational release factor 1 (MTRF1), nuclear gene encoding mitochondrial protein |
CNY 5256.00 |
