MYOM1 (NM_003803) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	SKELEMIN
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC303380 representing NM_003803. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCTTTGCCTTTTTATCAGAGGTGCCACCAGCACTATGATCTCAGCTACCGCAACAAGGACGTGCGC AGCACCGTGAGTCACTACCAGCGGGAGAAGAAACGCTCCGCCGTCTACACCCAGGGCTCCACGGCCTAC AGCAGCCGCTCCTCCGCCGCGCACCGCCGGGAGTCCGAGGCCTTCCGTCGGGCGTCCGCCTCCTCCTCC CAGCAGCAGGCCTCGCAGCACGCCCTGAGCTCTGAAGTCAGTCGGAAGGCAGCCTCAGCCTACGATTAT GGCTCCTCCCATGGACTTACAGATTCCAGTCTGCTGTTAGATGATTATTCATCCAAGTTGAGCCCCAAA CCAAAGAGAGCCAAGCACAGCCTACTGTCTGGAGAAGAGAAAGAAAATTTGCCCAGTGACTACATGGTA CCCATTTTCTCAGGACGTCAAAAGCATGTCAGTGGAATTACTGATACGGAAGAAGAAAGAATTAAAGAA GCTGCTGCTTATATAGCCCAGAGGAATCTTCTTGCTAGTGAGGAAGGAATCACAACATCTAAACAGTCC ACGGCATCCAAGCAGACCACGGCATCTAAGCAGTCCACGGCATCCAAGCAGTCCACAGCATCCAAGCAG TCCACGGCATCCAGGCAGTCCACGGCATCCAGGCAGTCTGTGGTTTCCAAACAGGCCACATCCGCTCTT CAACAGGAAGAAACTTCTGAAAAGAAGTCAAGGAAAGTTGTGATTCGAGAAAAGGCAGAACGCCTGTCC CTGAGGAAAACATTAGAAGAAACCGAGACATATCATGCCAAGCTGAATGAAGACCATCTTCTCCATGCT CCTGAGTTTATCATTAAACCTCGCTCCCACACGGTTTGGGAGAAGGAGAATGTAAAATTGCATTGCTCC ATAGCAGGCTGGCCAGAACCTCGTGTCACGTGGTATAAAAACCAGGTGCCAATAAATGTCCATGCAAAC CCTGGAAAGTATATTATTGAGAGTCGATATGGGATGCACACTCTGGAGATTAATGGATGTGATTTTGAA GATACAGCTCAGTACCGGGCCTCGGCGATGAATGTTAAAGGAGAGCTTTCGGCATATGCTTCAGTTGTG GTAAAAAGGTATAAGGGAGAGTTTGATGAGACTCGCTTCCACGCTGGGGCTTCCACCATGCCCCTCAGC TTTGGTGTGACCCCATATGGTTATGCATCCCGGTTTGAGATCCACTTTGATGACAAATTTGATGTGTCT TTTGGGAGAGAGGGAGAGACAATGAGTCTAGGCTGTCGTGTTGTCATCACTCCTGAAATTAAACATTTC CAGCCAGAGATCCAGTGGTACAGAAACGGAGTACCTCTTTCTCCATCAAAATGGGTGCAAACACTTTGG AGTGGAGAGCGGGCAACGCTGACATTTTCCCATCTCAACAAAGAAGATGAAGGCCTCTATACAATCCGT GTACGGATGGGAGAATATTATGAACAATATAGTGCTTATGTCTTTGTTCGAGATGCTGATGCAGAGATT GAAGGAGCCCCAGCTGCTCCCTTGGATGTGAAGTGCTTGGAGGCCAACAAAGATTATATCATCATCTCC TGGAAACAGCCAGCTGTCGATGGAGGGAGTCCTATTCTCGGATATTTTATTGATAAGTGTGAGGTGGGC ACAGATAGCTGGTCGCAGTGCAATGACACACCTGTGAAGTTTGCTCGTTTTCCTGTCACTGGATTGATC GAAGGTCGTTCCTATATCTTCCGAGTTCGAGCTGTGAATAAAATGGGAATAGGTTTCCCATCTCGAGTT TCCGAGCCCGTGGCTGCTCTGGATCCGGCTGAGAAAGCTAGACTTAAAAGTCGCCCCTCAGCACCCTGG ACTGGACAGATCATTGTTACTGAAGAGGAACCTTCAGAGGGTATTGTGCCTGGCCCCCCGACAGACCTC TCTGTCACTGAGGCCACCCGGAGCTATGTGGTGCTCAGCTGGAAGCCCCCTGGCCAGCGTGGTCATGAG GGCATTATGTACTTTGTGGAAAAGTGTGAGGCAGGAACAGAAAACTGGCAGCGAGTGAACACGGAGCTC CCTGTGAAGTCTCCCCGCTTTGCTCTGTTTGACTTGGCCGAGGGGAAATCCTACTGTTTCCGTGTCCGC TGTTCTAATTCTGCAGGAGTTGGTGAGCCCTCAGAGGCAACGGAGGTGACTGTGGTAGGGGACAAACTT GATATCCCCAAGGCTCCTGGCAAAATCATCCCAAGCAGAAACACAGACACCTCAGTGGTAGTTTCGTGG GAGGAGTCCAAAGATGCCAAAGAGCTGGTCGGGTACTACATAGAGGCGAGCGTTGCTGGCTCTGGCAAG TGGGAGCCCTGTAACAACAACCCCGTGAAGGGCTCACGATTCACTTGTCATGGATTAGTGACTGGTCAG AGTTATATTTTCCGGGTCAGAGCAGTCAATGCAGCTGGACTTAGTGAATATTCCCAGGATTCAGAAGCT ATTGAAGTCAAAGCTGCTATTGGGGGAGGAGTGTCTCCAGATGTGTGTCCCGCACTGAGCGATGAGCCT GGTGGACTAACCGCCTCCAGGGGGCGCGTGCATGAAGCCTCCCCGCCAACCTTCCAGAAAGATGCTTTG CTTGGCAGCAAACCTAACAAACCTTCACTACCCAGTAGCTCTCAAAACCTGGGCCAAACAGAAGTGAGT AAAGTAAGTGAAACAGTTCAGGAAGAGCTTACCCCGCCACCACAGAAAGCGGCTCCTCAGGGGAAAAGT AAGTCTGACCCCCTGAAAAAGAAGACAGACAGAGCACCACCATCTCCACCCTGTGATATCACCTGTCTT GAAAGTTTTCGTGACTCAATGGTTCTTGGATGGAAGCAACCAGATAAGATTGGAGGGGCAGAAATTACT GGCTATTATGTGAACTATCGCGAGGTCATTGATGGGGTACCAGGAAAATGGAGAGAAGCCAATGTCAAG GCTGTCAGTGAGGAGGCATACAAGATTAGCAACTTGAAGGAAAACATGGTGTATCAGTTCCAAGTGGCA GCCATGAACATGGCTGGGCTGGGCGCGCCCTCCGCAGTAAGCGAATGCTTCAAATGTGAAGAGTGGACC ATCGCCGTCCCAGGACCACCGCACAGTCTCAAGTGTAGTGAAGTCAGGAAAGACTCACTGGTTCTCCAG TGGAAGCCGCCAGTCCACTCCGGGCGGACTCCGGTCACTGGTTACTTCGTGGACTTGAAGGAGGCCAAG GCCAAAGAAGACCAGTGGCGAGGGCTCAATGAGGCGGCTATTAAAAACGTATACCTGAAGGTTCGAGGC CTCAAGGAGGGCGTCAGCTACGTGTTCCGTGTTCGAGCCATAAACCAGGCGGGAGTTGGGAAGCCATCT GACCTTGCTGGCCCTGTTGTGGCAGAGACCCGTCCAGGAACCAAAGAGGTTGTTGTAAATGTGGATGAT GATGGAGTCATTTCATTGAACTTCGAGTGTGATAAGATGACTCCAAAGTCCGAGTTCTCCTGGTCCAAA GATTATGTATCCACTGAGGACTCTCCACGATTGGAAGTCGAAAGCAAGGGCAACAAGACGAAAATGACC TTCAAAGACCTTGGGATGGATGACTTGGGTATTTACTCTTGCGATGTAACAGACACTGATGGAATAGCA TCAAGCTACTTAATAGATGAGGAAGAATTGAAACGTTTACTTGCTCTCAGCCATGAACACAAGTTCCCA ACTGTCCCAGTTAAATCAGAGTTGGCAGTTGAAATTTTGGAGAAAGGCCAGGTCCGGTTTTGGATGCAG GCTGAGAAACTGTCTGGCAATGCCAAAGTCAACTACATATTTAACGAGAAGGAAATTTTTGAAGGCCCG AAATATAAAATGCATATTGACCGAAACACTGGCATCATCGAAATGTTCATGGAAAAGCTACAGGATGAG GATGAGGGAACGTACACTTTCCAGCTTCAAGATGGAAAAGCAACTAACCATTCTACTGTTGTTCTCGTT GGAGATGTTTTCAAAAAGCTCCAGAAAGAAGCTGAATTCCAGCGGCAAGAATGGATCAGGAAACAAGGT CCTCACTTTGTTGAGTATTTGAGCTGGGAAGTGACTGGTGAATGTAATGTACTATTGAAATGCAAGGTG GCAAATATTAAGAAGGAGACTCATATTGTGTGGTACAAAGATGAGAGGGAGATATCAGTGGATGAAAAG CATGACTTTAAGGATGGTATATGTACCCTGCTTATAACAGAGTTTTCCAAGAAAGATGCTGGGATTTAT GAAGTTATCCTGAAAGATGACCGAGGAAAAGATAAGAGCAGACTGAAGCTTGTGGATGAAGCCTTTAAG GAACTGATGATGGAAGTATGCAAAAAAATAGCTTTGTCTGCTACAGACCTGAAAATCCAGAGCACAGCC GAGGGCATCCAACTGTACTCTTTTGTAACTTACTATGTGGAGGATTTGAAAGTTAACTGGTCCCACAAT GGGTCCGCCATTAGGTACTCAGACAGAGTTAAGACCGGGGTCACTGGAGAGCAGATCTGGCTACAAATC AACGAGCCCACCCCGAATGACAAAGGGAAGTATGTCATGGAGCTCTTTGATGGCAAAACTGGACATCAG AAGACAGTGGATCTCTCTGGACAAGCATACGATGAGGCCTATGCTGAATTCCAGAGGTTGAAACAAGCT GCCATTGCCGAGAAAAATCGTGCCCGGGTGTTGGGAGGTCTCCCAGACGTGGTCACCATCCAGGAGGGG AAGGCCCTTAATCTCACTTGCAACGTGTGGGGAGACCCGCCTCCGGAGGTGTCGTGGTTGAAGAACGAG AAGGCCCTGGCCTCAGACGACCACTGCAACCTCAAGTTCGAGGCTGGGAGGACCGCGTACTTCACCATC AACGGCGTGAGCACCGCTGACTCGGGCAAATACGGGCTGGTTGTGAAGAACAAGTATGGCTCGGAGACC AGCGACTTCACCGTCAGCGTGTTCATCCCAGAGGAGGAGGCGAGGATGGCCGCCTTGGAGTCCCTGAAA GGTGGCAAGAAGGCCAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_003803
Insert Size	5058 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_003803.3
RefSeq Size	5848 bp
RefSeq ORF	5058 bp
Locus ID	8736
UniProt ID	P52179
MW	187.6 kDa
Gene Summary	The giant protein titin, together with its associated proteins, interconnects the major structure of sarcomeres, the M bands and Z discs. The C-terminal end of the titin string extends into the M line, where it binds tightly to M-band constituents of apparent molecular masses of 190 kD (myomesin 1) and 165 kD (myomesin 2). This protein, myomesin 1, like myomesin 2, titin, and other myofibrillar proteins contains structural modules with strong homology to either fibronectin type III (motif I) or immunoglobulin C2 (motif II) domains. Myomesin 1 and myomesin 2 each have a unique N-terminal region followed by 12 modules of motif I or motif II, in the arrangement II-II-I-I-I-I-I-II-II-II-II-II. The two proteins share 50% sequence identity in this repeat-containing region. The head structure formed by these 2 proteins on one end of the titin string extends into the center of the M band. The integrating structure of the sarcomere arises from muscle-specific members of the superfamily of immunoglobulin-like proteins. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) represents the longer transcript, and encodes the longer isoform (a).

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