DGKD (NM_003648) Human Untagged Clone
CAT＃: SC303359
DGKD (untagged)-Human diacylglycerol kinase, delta 130kDa (DGKD), transcript variant 1
CNY 22520.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DGK-delta; dgkd-2; DGKdelta |
| Vector | pCMV6 series |
| Sequence Data |
>NCBI ORF sequence for NM_003648, the custom clone sequence may differ by one or more nucleotides
ATGAATATGTTTCTGTACTTTCAGACCATCATCAAAGAGGGGATGCTGACCAAACAGAAC AATTCATTCCAGCGATCAAAAAGGAGATACTTTAAGCTTCGAGGGCGAACGCTTTACTAT GCCAAAACGGCAAAGTCAATCATATTTGATGAGGTGGATCTGACAGATGCCAGCGTAGCT GAATCCAGTACCAAAAACGTCAACAACAGTTTTACGGTCATAACTCCATGCAGGAAGCTC ATCTTGTGTGCTGATAACAGAAAAGAAATGGAAGATTGGATTGCAGCATTAAAGACTGTG CAGAACAGGGAGCACTTTGAGCCCACCCAGTACAGCATGGACCACTTCTCAGGGATGCAC AATTGGTACGCCTGTTCCCACGCGAGGCCGACCTACTGCAATGTGTGCCGTGAGGCTCTG TCTGGGGTCACGTCGCACGGGCTGTCCTGCGAGGTGTGCAAATTTAAGGCCCACAAGCGC TGTGCTGTGCGTGCAACCAATAACTGCAAGTGGACCACACTGGCCTCGATCGGGAAGGAC ATCATTGAAGATGCAGATGGGATTGCAATGCCCCACCAGTGGTTGGAAGGAAACCTACCT GTGAGCGCCAAGTGCACTGTGTGCGACAAGACCTGTGGCAGTGTGCTGCGCCTGCAGGAC TGGCGCTGCCTCTGGTGCAAGGCCATGGTTCACACATCGTGTAAAGAATCCTTGCTGACC AAGTGCCCACTTGGCCTGTGCAAAGTGTCAGTCATCCCACCCACGGCTCTCAACAGCATC GACTCCGATGGGTTCTGGAAGGCCAGCTGTCCTCCTTCTTGCACAAGCCCACTGTTGGTC TTCGTCAATTCAAAAAGTGGGGACAACCAGGGTGTGAAGTTCCTCAGAAGATTCAAACAG CTACTAAACCCCGCCCAGGTCTTCGACCTCATGAACGGAGGCCCACACCTCGGCTTACGG TTATTCCAGAAGTTTGACACATTCCGGATTCTGGTTTGTGGCGGGGATGGAAGTGTTGGC TGGGTCCTCTCCGAAATCGACAGCCTCAACCTTCATAAACAGTGTCAGCTGGGAGTGCTG CCGCTCGGCACAGGGAACGACTTGGCCCGAGTACTGGGCTGGGGCTCAGCCTGCGATGAC GACACCCAGCTCCCCCAGATCTTGGAGAAGTTGGAGAGAGCCAGCACCAAGATGCTGGAC AGGTGGAGCGTCATGGCATACGAGGCCAAGCTCCCCCGGCAGGCCTCCTCCTCTACCGTC ACCGAAGACTTCAGCGAGGATTCCGAGGTACAGCAGATTCTCTTCTATGAAGACTCGGTT GCAGCCCACCTTTCTAAAATCCTCACCTCGGACCAGCACTCGGTGGTCATCTCCTCGGCC AAAGTGCTCTGTGAGACGGTGAAGGACTTCGTGGCACGGGTGGGGAAGGCCTATGAGAAG ACGACCGAGAGCTCGGAGGAGTCAGAGGTCATGGCCAAGAAGTGCTCTGTCCTGAAAGAG AAGCTGGATTCCCTTCTCAAGACCTTGGACGATGAGTCCCAGGCCTCGTCCTCTCTGCCC AACCCGCCCCCCACCATTGCCGAGGAGGCTGAAGATGGAGATGGGTCGGGCAGCATCTGC GGTTCCACCGGAGACCGCTTGGTGGCATCAGCTTGCCCGGCCCGGCCGCAGATATTCCGG CCTCGAGAACAGCTCATGCTGAGAGCCAACAGCCTGAAGAAAGCAATTCGTCAGATCATA GAACACACAGAAAAAGCTGTCGATGAGCAGAATGCCCAGACCCAGGAGCAGGAGGGCTTC GTCCTGGGCCTCTCTGAGTCAGAGGAGAAGATGGACCACAGAGTGTGCCCACCACTGTCC CACAGCGAGAGCTTCGGGGTCCCCAAGGGGAGGAGCCAGCGCAAAGTGTCGAAATCTCCG TGTGAAAAGCTGATCAGCAAAGGGAGTCTGTCCCTAGGCAGTTCTGCTTCCCTTCCGCCC CAGCCGGGAAGCCGGGACGGCCTGCCTGCGCTCAACACCAAGATCCTGTACCCAAATGTC CGGGCTGGAATGTCTGGTTCCTTACCCGGTGGCTCAGTCATCAGTCGCCTGTTAATTAAT GCTGATCCCTTCAACTCTGAACCAGAAACCCTAGAGTATTACACGGAGAAATGTGTCATG AACAACTATTTTGGCATTGGCCTGGATGCGAAGATATCCCTGGACTTTAACAACAAGCGC GATGAGCACCCAGAGAAGTGCAGGAGCCGAACCAAGAACATGATGTGGTATGGAGTTCTT GGAACCAAAGAGTTGCTGCACAGAACCTACAAGAACCTGGAGCAAAAGGTCTTGCTGGAG TGTGACGGGCGACCCATCCCACTCCCCAGTCTTCAGGGAATTGCTGTCCTTAACATTCCC AGCTATGCCGGAGGAACCAACTTCTGGGGGGGTACCAAGGAAGATGATACTTTCGCAGCT CCATCATTCGATGACAAGATTCTGGAGGTGGTCGCCGTGTTCGGCAGCATGCAGATGGCC GTCTCTCGAGTCATCAGGCTACAGCATCATCGGATCGCCCAGTGTCGCACGGTGAAGATC TCCATCCTTGGGGATGAGGGCGTGCCTGTGCAGGTGGACGGAGAGGCCTGGGTCCAGCCG CCAGGGTACATTCGGATTGTCCACAAGAACCGGGCACAGACACTGACCAGAGACAGGGCA TTTGAGAGCACCCTGAAGTCCTGGGAAGACAAGCAGAAGTGCGAGCTGCCCCGCCCTCCA TCCTGTTCCCTGCACCCGGAGATGCTGTCCGAGGAGGAGGCCACCCAGATGGACCAGTTT GGGCAGGCAGCAGGGGTCCTCATTCACAGTATCCGAGAAATAGCTCAGTCTCACCGGGAC ATGGAGCAGGAACTGGCCCACGCCGTCAATGCCAGCTCCAAGTCCATGGACCGTGTGTAT GGCAAGCCCAGAACCACAGAGGGGCTCAACTGCAGCTTCGTCCTGGAAATGGTGAATAAC TTCAGAGCTCTGCGCAGTGAGACGGAGCTGCTGCTGTCTGGGAAGATGGCCCTGCAGCTG GATCCGCCTCAGAAGGAGCAGCTGGGGAGTGCTCTTGCCGAGATGGACCGACAGCTCAGG AGGCTGGCAGACACCCCGTGGCTCTGCCAGTCCGCAGAGCCCGGCGACGAAGAGAGTGTG ATGCTGGATCTTGCCAAGCGCAGTCGCAGTGGTAAATTCCGCCTCGTGACCAAGTTTAAA AAGGAGAAAAACAACAAGAACAAAGAAGCTCACAGTAGCCTGGGAGCCCCGGTTCACCTC TGGGGGACAGAGGAGGTTGCTGCCTGGCTGGAGCACCTCAGTCTCTGTGAGTATAAGGAC ATCTTCACACGGCACGACATCCGGGGCTCTGAGCTCCTGCACCTGGAGCGGAGGGACCTC AAGGACCTGGGCGTGACCAAGGTGGGCCACATGAAGAGGATCCTGTGTGGCATCAAGGAG CTGAGCCGCAGCGCCCCCGCCGTCGAGGCCTAG |
| Restriction Sites | Please inquire |
| ACCN | NM_003648 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003648.2, NP_003639.2 |
| RefSeq Size | 6229 bp |
| RefSeq ORF | 3513 bp |
| Locus ID | 8527 |
| UniProt ID | Q16760 |
| Protein Families | Druggable Genome |
| Protein Pathways | Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system |
| Gene Summary | This gene encodes a cytoplasmic enzyme that phosphorylates diacylglycerol to produce phosphatidic acid. Diacylglycerol and phosphatidic acid are two lipids that act as second messengers in signaling cascades. Their cellular concentrations are regulated by the encoded protein, and so it is thought to play an important role in cellular signal transduction. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) differs in the 5' UTR and uses a different in-frame start codon, compared to variant 2. The encoded isoform (1) has a shorter, distinct N-terminus, compared to isoform 2. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216667 | DGKD (Myc-DDK-tagged)-Human diacylglycerol kinase, delta 130kDa (DGKD), transcript variant 1 |
CNY 8960.00 |
|
| RC216667L3 | Lenti-ORF clone of DGKD (Myc-DDK-tagged)-Human diacylglycerol kinase, delta 130kDa (DGKD), transcript variant 1 |
CNY 13590.00 |
|
| RC216667L4 | Lenti-ORF clone of DGKD (mGFP-tagged)-Human diacylglycerol kinase, delta 130kDa (DGKD), transcript variant 1 |
CNY 13590.00 |
|
| RG216667 | DGKD (tGFP-tagged) - Human diacylglycerol kinase, delta 130kDa (DGKD), transcript variant 1 |
CNY 12830.00 |
