ZAN (NM_003386) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC303298 representing NM_003386. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGTTCCTCCAGTCTGGACTCTGCTGCTTCTGGTGGGGGCTGCCCTTTTCAGGAAAGAGAAGCCTCCG GACCAGAAGCTGGTTGTTCGCAGCTCTAGGGACAACTATGTCCTCACCCAGTGTGATTTTGAGGATGAC GCCAAACCCCTCTGTGACTGGTCCCAAGTGTCCGCAGACGATGAAGACTGGGTTCGAGCCAGTGGGCCC TCTCCCACCGGCTCCACCGGGGCCCCCGGGGGGTACCCTAACGGAGAGGGCAGCTATCTGCATATGGAA TCGAACAGCTTCCACCGTGGGGGAGTGGCCCGCCTGCTCAGCCCCGACCTATGGGAGCAAGGCCCCCTC TGTGTGCACTTTGCCCACCACATGTTCGGGCTGTCTTGGGGCGCCCAGCTCAGGCTGCTGCTGCTCTCG GGTGAAGAGGGCCGCCGCCCCGATGTGCTCTGGAAACACTGGAACACCCAGAGACCCTCCTGGATGCTC ACCACCGTCACTGTGCCCGCAGGGTTCACCCTGCCCACCCGGCTGATGTTTGAGGGAACACGGGGTAGC ACTGCCTACCTGGACATCGCCCTGGATGCCCTCTCTATCCGCCGGGGCTCCTGTAATCGCGTCTGTATG ATGCAAACATGCAGCTTTGACATTCCAAATGACCTCTGTGACTGGACCTGGATCCCAACTGCCTCCGGG GCCAAGTGGACTCAGAAGAAAGGGTCATCAGGAAAGCCAGGCGTGGGGCCTGATGGCGACTTCTCTAGC CCTGGTAGTGGCTGCTACATGCTCCTGGACCCCAAGAATGCAAGACCTGGGCAGAAAGCTGTCCTCCTG AGCCCCGTGAGCCTGTCCTCTGGCTGTCTGAGCTTTTCCTTCCACTACATCCTCCGGGGCCAGTCTCCT GGTGCAGCCCTCCACATTTATGCTTCAGTCTTGGGGAGTATCCGGAAACACACTCTCTTCTCAGGACAA CCTGGGCCCAACTGGCAGGCTGTTTCTGTCAATTACACAGCCGTGGGACGGATACAGTTTGCCGTGGTA GGCGTTTTTGGAAAGACCCCAGAGCCAGCTGTGGCAGTTGATGCAACCAGCATTGCTCCTTGTGGGGAG GGTTTTCCTCAGTGTGACTTTGAAGACAACGCCCATCCCTTCTGTGACTGGGTCCAGACTTCCGGGGAT GGTGGACACTGGGCCCTCGGACATAAAAATGGACCCGTCCATGGCATGGGCCCTGCGGGAGGTTTCCCT AATGCAGGGGGTCACTATATCTACCTTGAGGCTGACGAGTTCTCCCAGGCAGGCCAGTCAGTCAGACTG GTGAGCCGGCCCTTCTGCGCCCCAGGTGACATCTGCGTGGAGTTCGCATACCACATGTATGGCCTTGGG GAGGGTACTATGCTCGAACTCCTCCTGGGAAGTCCTGCGGGGAGTCCCCCGATTCCTCTCTGGAAACGC GTGGGGTCTCAGCGCCCTTACTGGCAGAACACCTCCGTCACCGTCCCCTCAGGACACCAACAGCCCATG CAGCTTATTTTCAAGGGCATCCAGGGAAGCAACACGGCCTCTGTGGTTGCTATGGGTTTCATCTTGATC AATCCTGGGACTTGTCCAGTAAAAGTGCTACCAGAGCTTCCTCCCGTATCTCCAGTTTCTTCCACTGGC CCTTCTGAAACCACTGGCCTCACAGAAAACCCTACAATCTCCACCAAGAAACCTACAGTTTCCATAGAA AAACCCAGTGTCACCACAGAAAAGCCCACAGTCCCCAAAGAAAAGCCCACCATTCCCACAGAAAAACCC ACCATCTCCACAGAAAAACCCACCATTCCTTCAGAAAAACCCAACATGCCCTCAGAAAAACCCACCATT CCCTCAGAAAAACCCACCATCCTCACAGAAAAACCCACCATTCCCTCAGAAAAACCCACCATTCCCTCA GAAAAACCCACCATTTCCACAGAAAAACCCACCGTCCCCACAGAAGAGCCCACCACCCCCACTGAGGAG ACCACCACCTCCATGGAAGAGCCTGTCATCCCTACAGAAAAACCCAGCATCCCTACAGAAAAACCCAGC ATCCCCACGGAAAAACCCACCATCTCCATGGAAGAGACTATCATCTCCACAGAAAAACCCACCATCTCC CCAGAAAAACCCACCATCCCCACAGAAAAACCCACCATCCCCACAGAAAAATCCACCATCTCCCCAGAA AAACCCACCACCCCCACAGAAAAACCCACCATCCCCACAGAAAAACCCACCATCTCCCCAGAAAAACCC ACCACCCCCACAGAAAAACCCACCATCTCCCCAGAAAAACTCACCATCCCCACAGAAAAACCCACCATC CCCACAGAAAAACCCACCATTCCCACAGAAAAACCCACCATCTCCACAGAAGAGCCCACCACCCCCACT GAGGAGACCACCATCTCCACAGAAAAACCCAGCATCCCCATGGAAAAACCCACTCTCCCCACTGAAGAA ACCACCACCTCTGTTGAAGAGACTACCATCTCTACAGAAAAACTCACCATCCCCATGGAAAAACCCACC ATCTCCACAGAAAAACCCACCATCCCCACAGAAAAACCCACCATCTCCCCAGAAAAACTCACCATCCCC ACGGAAAAACTCACCATCCCCACGGAAAAACCCACCATCCCCATTGAAGAGACTACCATCTCCACAGAA AAACTCACCATCCCCACAGAAAAACCCACCATCTCCCCAGAAAAACCCACCATCTCCACGGAAAAACCC ACCATCCCCACGGAAAAACCCACCATCCCCACTGAAGAGACTACCATCTCCACAGAAAAACTCACCATC CCCACAGAAAAACCCACCATCTCCCCAGAAAAACTCACCATCCCCACAGAAAAACCCACCATCTCCACG GAAAAACCCACCATCCCCACGGAAAAACTTACCATCCCCACGGAAAAACCCACCATCCCCACAGAAAAA CCCACCATTCCCACAGAGAAGCTCACAGCCCTGAGGCCACCCCATCCCAGCCCCACAGCCACTGGGCTG GCAGCCTTGGTGATGTCTCCACATGCTCCAAGTACCCCTATGACCAGTGTGATTCTGGGCACTACCACA ACCTCCAGATCCAGTACAGAGCGCTGCCCTCCAAATGCCCGCTACGAATCCTGTGCTTGTCCTGCTTCG TGCAAGAGCCCCAGGCCTAGCTGTGGGCCCCTCTGTCGGGAGGGCTGTGTCTGCAACCCTGGCTTTTTG TTTAGTGACAACCACTGCATCCAGGCCTCTTCCTGCAATTGCTTCTACAACAACGACTACTATGAGCCT GGGGCAGAGTGGTTCAGCCCCAACTGCACAGAACATTGCCGCTGCTGGCCCGGCAGTCGGGTCGAGTGC CAGATCTCTCAGTGTGGGACACACACCGTGTGCCAGCTTAAGAATGGCCAGTATGGATGCCACCCCTAC GCAGGCACTGCCACCTGCTTGGTCTACGGAGACCCTCATTATGTCACCTTTGACGGGAGGCACTTTGGC TTCATGGGCAAGTGCACTTACATCTTGGCCCAGCCCTGTGGCAACTCAACAGACCCATTCTTCAGGGTG ACAGCCAAGAATGAGGAGCAGGGACAGGAAGGCGTGTCCTGCCTGAGCAAAGTCTACGTGACCCTGCCC GAGAGCACCGTCACCCTGCTTAAGGGCAGACGCACTCTGGTTGGGGGTCAGCAAGTTACTCTCCCAGCC ATACCCTCTAAAGGCGTCTTCCTGGGTGCAAGCGGGCGGTTTGTGGAGCTGCAGACGGAGTTCGGTTTG CGGGTGAGATGGGATGGTGACCAGCAGCTGTATGTTACTGTGTCCAGCACATACTCTGGCAAACTCTGT GGTTTGTGTGGGAACTATGACGGCAACAGTGACAATGACCACCTGAAGTTGGACGGCAGCCCAGCAGGA GACAAGGAGGAGCTGGGGAACAGCTGGCAGACGGACCAGGACGAGGACCAGGAGTGTCAGAAGTACCAG GTGGTGAATTCCCCGTCTTGTGATTCATCTCTGCAGAGCAGCATGTCGGGGCCAGGGTTCTGTGGACGG CTGGTCGACACTCATGGCCCATTTGAGACATGCCTGCTGCACGTGAAGGCCGCTTCCTTCTTCGACAGC TGCATGCTTGATATGTGCGGATTCCAGGGGCTGCAGCACCTGCTGTGCACACACATGTCCACCATGACC ACCACCTGCCAGGACGCAGGCCACGCTGTGAAGCCCTGGAGGGAACCCCACTTCTGCCCAATGGCCTGC CCGCCCAACAGCAAGTACTCCCTGTGTGCGAAGCCATGCCCTGACACCTGCCATTCAGGATTCTCCGGC ATGTTCTGCTCAGACCGGTGCGTGGAGGCCTGTGAATGCAATCCGGGCTTCGTCCTCAGTGGCCTCGAG TGCATACCTCGCTCCCAGTGTGGGTGCCTCCACCCTGCAGGCAGCTACTTCAAGGTAGGGGAGCGGTGG TACAAGCCAGGCTGCAAAGAGTTGTGCGTCTGTGAAAGCAACAACAGAATTCGCTGCCAGCCCTGGAGG TGTAGGGCCCAGGAGTTCTGTGGCCAACAGGATGGTATCTATGGCTGCCATGCCCAAGGTGCCGCCACC TGCACAGCCTCGGGTGACCCCCACTACCTGACCTTCGATGGCGCCTTGCACCACTTCATGGGCACCTGC ACCTATGTCCTGACCCGGCCTTGCTGGTCCAGGTCCCAAGACAGCTATTTTGTTGTGAGCGCCACCAAC GAGAACCGCGGGGGGATCCTGGAGGTCTCCTACATCAAAGCCGTCCACGTGACAGTCTTTGACCTCAGC ATCTCACTGCTCAGAGGCTGTAAGGTCATGCTGAATGGCCATCGGGTGGCCCTACCTGTGTGGCTTGCA CAAGGCCGGGTGACCATAAGGCTCAGCAGCAACCTCGTCCTCCTCTACACGAACTTTGGGCTCCAAGTT CGCTACGACGGGAGCCACTTGGTGGAAGTGACAGTCCCCTCCTCCTATGGCGGCCAGCTCTGTGGGCTG TGTGGGAACTACAACAACAACAGCTTGGATGACAACCTGCGCCCCGACAGAAAGCTTGCAGGCGATTCC ATGCAGCTGGGGGCCGCCTGGAAGTTACCTGAATCCTCTGAACCTGGCTGTTTCCTTGTGGGTGGCAAG CCCTCCAGCTGCCAGGAGAACAGCATGGCAGACGCCTGGAACAAGAACTGTGCGATCTTAATAAACCCT CAGGGACCCTTCTCTCAATGTCACCAGGTGGTGCCTCCCCAGTCCAGCTTTGCCAGTTGCGTGCATGGT CAGTGTGGGACCAAGGGCGACACCACAGCCCTGTGCCGCTCCCTGCAGGCCTACGCGTCCCTGTGTGCC CAGGCTGGCCAGGCCCCTGCCTGGCGGAACAGAACCTTCTGCCCTATGAGGTGCCCACCTGGCAGCAGC TACAGCCCCTGCAGCAGCCCCTGCCCAGACACCTGCAGCAGCATAAACAACCCGAGGGACTGCCCCAAA GCACTGCCCTGTGCTGAGAGCTGTGAATGTCAGAAAGGCCACATCTTGAGTGGAACCTCCTGCGTGCCC CTTGGCCAGTGTGGCTGCACTGACCCAGCGGGCTCCTACCACCCGGTCGGGGAGCGCTGGTACACAGAG AACACCTGCACCAGGCTCTGCACCTGCTCCGTCCACAACAACATCACCTGCTTCCAGAGCACCTGCAAA CCCAACCAGATATGCTGGGCCCTGGATGGGCTGCTCCATTGTCGGGCCTCAGGTGTGGGAGTGTGTCAG CTCCCAGGGGAGTCCCACTACGTGAGCTTTGATGGTAGTAACCATTCTATCCCGGACGCCTGCACTCTT GTCCTGGTGAAAGTGTGCCACCCCGCCATGGCCTTGCCCTTCTTCAAGATCAGTGCCAAGCATGAGAAG GAGGAAGGTGGAACTGAGGCTTTCCGCCTTCATGAGGTCTACATTGACATCTACGATGCCCAGGTCACC CTGCAGAAGGGCCACCGTGTGCTAATCAACAGCAAACAGGTCACCCTCCCCGCCATCTCCCAGATCCCT GGGGTCAGTGTCAAGTCCAGCAGCATCTACAGCATTGTTAACATCAAGATCGGGGTGCAAGTCAAGTTT GACGGGAATCATCTCTTAGAGATTGAAATCCCCACAACCTACTATGGAAAGGTCTGCGGCATGTGTGGG AACTTCAATGATGAGGAAGAGGACGAACTAATGATGCCCAGCGATGAAGTAGCAAATAGTGACAGTGAA TTTGTGAACAGTTGGAAAGATAAGGACATTGACCCAAGTTGTCAGAGTCTCCTGGTAGATGAGCAGCAG ATTCCAGCGGAACAGCAGGAGAACCCGAGTGGAAACTGCAGGGCGGCCGACCTCCGCAGGGCGCGGGAA AAGTGCGAGGCAGCGCTCCGGGCTCCTGTGTGGGCCCAGTGCGCCTCCCGCATAGACCTCACGCCCTTC CTGGTGGACTGTGCAAACACCCTCTGTGAGTTCGGAGGTCTCTACCAGGCCCTCTGCCAGGCTCTGCAA GCCTTCGGGGCCACCTGCCAGAGCCAGGGGCTCAAGCCCCCACTCTGGAGAAACAGCAGCTTCTGCCCT CTGGAATGCCCTGCCTACAGCAGCTACACCAACTGCCTTCCCTCCTGCTCACCCTCCTGCTGGGACCTG GATGGCCGGTGTGAGGGCGCCAAAGTCCCCTCTGCCTGCGCTGAGGGCTGCATTTGTCAGCCCGGCTAT GTGCTGAGTGAAGACAAGTGTGTCCCCAGAAGTCAGTGTGGCTGCAAGGATGCCCATGGTGGCTCCATC CCTCTGGGCAAGAGCTGGGTCTCCAGCGGTTGCACGGAGAAGTGTGTCTGCACGGGAGGAGCCATTCAG TGCGGGGACTTCCGATGCCCCTCTGGGTCCCACTGCCAGCTCACTTCCGACAACAGCAACAGCAATTGT GTCTCAGACAAGTCTGAACAATGCTCAGTCTATGGCGACCCCCGTTACCTCACATTTGACGGCTTCAGC TACCGCTTGCAAGGCCGCATGACCTATGTTCTGATCAAGACTGTGGACGTACTGCCTGAGGGGGTGGAG CCCCTCCTCGTGGAAGGACGCAACAAGATGGATCCGCCCAGGAGCTCCATCTTCTTGCAGGAAGTGATT ACCACCGTCTACGGCTATAAAGTGCAGCTCCAAGCTGGTCTGGAGCTTGTGGTCAACAACCAGAAGATG GCCGTCCCCTACAGGCCAAATGAACACCTGCGGGTCACCCTGTGGGGCCAACGGCTCTACCTGGTCACC GACTTTGAGCTGGTCGTCAGCTTTGGTGGAAGGAAAAATGCAGTGATCTCCCTACCCAGCATGTACGAG GGGCTTGTGAGTGGCCTGTGCGGAAACTACGACAAGAACCGCAAGAATGACATGATGCTGCCCAGTGGC GCCCTGACCCAGAACCTCAACACCTTTGGCAACAGCTGGGAGGTGAAGACCGAGGACGCACTCCTGCGC TTCCCCAGGGCTATACCAGCGGAGGAGGAGGGACAAGGGGCGGAGCTGGGCCTCCGCACGGGCCTCCAA GTGTCCGAATGTAGCCCGGAGCAGCTGGCGAGCAACAGCACCCAGGCCTGTAGGGTGCTGGCAGACCCC CAGGGCCCCTTTGCTGCCTGTCACCAGACGGTGGCCCCAGAGCCCTTCCAAGAGCACTGCGTGCTGGAT CTGTGCTCTGCTCAGGACCCAAGAGAGCAAGAGGAGCTGCGTTGCCAGGTCCTCAGTGGCCATGGAGTG TCCAGCAGGTACCATATATCAGAGCTGTATGACACCCTGCCCAGCATCCTGTGCCAACCTGGCAGACCC CGGGGACTGCGAGGGCCCCTGCGTGGAAGGCTGCGCCAGCATCCCAGGCTATGCCTACAGTGGCACCCA GAGCCTCCCCTGGCTGACTGTGGCTGCACCAGCAATGGCATCTACTACCAGCTGGGCAGCAGCTTTCTG ACTGAGGACTGCTCTCAGCGGTGCACCTGTGCCAGCTCACGGATCCTGCTGTGTGAGCCCTTCAGCTGC AGAGCGGGGGAGGTCTGCACCCTGGGGAACCACACCCAAGGCTGCTTTCCAGAAAGCCCGTGTCTGCAG AACCCCTGTCAGAATGACGGGCAGTGTCGGGAGCAGGGAGCCACCTTCACCTGCGAGTGTGAAGTTGGT TACGGGGGAGGCCTGTGTATGGAGCCTCGAGATGCGCCACCTCCCAGAAAGCCAGCATCTAACCTGGTG GGCGTCCTACTGGGACTGCTGGTGCCTGTGGTGGTCGTACTACTGGCCGTGACCAGAGAGTGCATTTAC AGAACGAGGAGGAAGAGAGAGAAAACGCAGGAGGGAGACAGACTGGCCAGGCTGGTGGACACAGATACT GTTCTGGACTGTGCCTGTTAA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_003386
Insert Size	8439 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_003386.2
RefSeq Size	8689 bp
RefSeq ORF	8439 bp
Locus ID	7455
UniProt ID	Q9Y493
Protein Families	Druggable Genome, Transmembrane
MW	305.6 kDa
Gene Summary	This gene encodes a protein that functions in the species specificity of sperm adhesion to the egg zona pellucida. The encoded protein is located in the acrosome and may be involved in signaling or gamete recognition. An allelic polymorphism in this gene results in both functional and frameshifted alleles; the reference genome represents the functional allele. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2015] Transcript Variant: This variant (3) encodes the longer isoform (3).

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