SPAG4 (NM_003116) Human Untagged Clone
CAT＃: SC303276
SPAG4 (untagged)-Human sperm associated antigen 4 (SPAG4)
CNY 7030.00
Product images
CNY 1999.00
CNY 2700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CT127; SUN4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC303276 representing NM_003116.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCGGCGAAGCTCCCGCCCGGGCTCGGCCTCGTCCTCGCGCAAGCACACGCCCAACTTTTTCAGCGAG AACAGCTCAATGAGCATCACCTCGGAGGACAGCAAAGGGCTCCGGTCAGCGGAGCCCGGGCCTGGGGAG CCCGAGGGCAGAAGAGCCCGGGGCCCGAGCTGCGGTGAGCCCGCCTTGAGCGCGGGAGTGCCCGGAGGA ACCACATGGGCAGGAAGCTCTCAGCAGAAGCCAGCGCCTCGGAGCCACAACTGGCAGACAGCCTGTGGC GCGGCAACCGTGAGGGGCGGGGCCTCGGAACCGACTGGGTCTCCAGTAGTCTCTGAGGAGCCGCTCGAC CTTCTCCCGACCCTGGATCTGAGGCAGGAGATGCCTCCCCCGCGGGTGTTCAAGAGCTTTCTGAGCCTG CTCTTCCAGGGGCTGAGCGTGTTGTTATCCCTGGCAGGAGACGTGCTGGTCAGCATGTACAGGGAGGTC TGTTCCATCCGCTTCCTGTTCACGGCTGTGTCGCTGCTGAGCCTCTTTCTGTCAGCATTCTGGCTGGGG CTTCTGTACCTGGTCTCTCCTTTGGAGAATGAACCTAAGGAGATGCTGACTCTAAGTGAGTACCACGAG CGCGTGCGCTCCCAGGGGCAGCAGCTGCAGCAGCTCCAGGCCGAGCTGGATAAACTCCACAAGGAGGTG TCCACTGTTCGGGCAGCCAACAGCGAGAGAGTGGCCAAGCTCGTGTTCCAGAGGCTGAATGAGGATTTT GTGCGGAAGCCCGACTATGCTTTGAGCTCTGTGGGAGCCTCCATCGACCTGCAGAAGACATCCCACGAT TACGCAGACAGGAACACTGCCTACTTCTGGAATCGCTTCAGCTTCTGGAACTACGCACGGCCGCCCACG GTTATCCTGGAGCCCCACGTGTTCCCTGGGAATTGCTGGGCTTTTGAAGGCGACCAAGGCCAGGTGGTG ATCCAACTGCCGGGCCGAGTGCAGCTGAGCGACATCACTCTGCAGCATCCACCGCCCAGCGTGGAGCAC ACCGGAGGAGCCAACAGCGCCCCCCGCGATTTCGCGGTCTTTGGCCTCCAGGTTTATGATGAAACTGAA GTTTCCTTGGGGAAATTCACCTTCGATGTTGAGAAATCGGAGATTCAGACTTTCCACCTGCAGAATGAC CCCCCAGCTGCCTTTCCCAAGGTGAAGATCCAGATTCTAAGCAACTGGGGCCACCCCCGTTTCACGTGC TTGTATCGAGTCCGTGCCCACGGTGTGCGAACCTCAGAGGGGGCAGAGGGCAGTGCACAGGGGCCCCAT TAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_003116 |
| Insert Size | 1314 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003116.2 |
| RefSeq Size | 1479 bp |
| RefSeq ORF | 1314 bp |
| Locus ID | 6676 |
| UniProt ID | Q9NPE6 |
| Protein Families | Transmembrane |
| MW | 48.2 kDa |
| Gene Summary | The mammalian sperm flagellum contains two cytoskeletal structures associated with the axoneme: the outer dense fibers surrounding the axoneme in the midpiece and principal piece and the fibrous sheath surrounding the outer dense fibers in the principal piece of the tail. Defects in these structures are associated with abnormal tail morphology, reduced sperm motility, and infertility. In the rat, the protein encoded by this gene associates with an outer dense fiber protein via a leucine zipper motif and localizes to the microtubules of the manchette and axoneme during sperm tail development. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015] Transcript Variant: This variant (1) encodes the longer isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214367 | SPAG4 (Myc-DDK-tagged)-Human sperm associated antigen 4 (SPAG4) |
CNY 3656.00 |
|
| RC214367L3 | Lenti ORF clone of Human sperm associated antigen 4 (SPAG4), Myc-DDK-tagged |
CNY 5890.00 |
|
| RC214367L4 | Lenti ORF clone of Human sperm associated antigen 4 (SPAG4), mGFP tagged |
CNY 5890.00 |
|
| RG214367 | SPAG4 (tGFP-tagged) - Human sperm associated antigen 4 (SPAG4) |
CNY 5256.00 |
