STIL (NM_003035) Human Untagged Clone
CAT＃: SC303265
STIL (untagged)-Human SCL/TAL1 interrupting locus (STIL), transcript variant 2
CNY 10450.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MCPH7; SIL |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC303265 representing NM_003035.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGCCTATATATCCTTTTGCACGGCCCCAGATGAATACCAGGTTTCCTTCAAGCAGGATGGTACCT TTCCACTTTCCTCCATCAAAATGTGCACTTTGGAACCCAACGCCAACTGGAGATTTCATCTACTTACAT CTCAGTTACTACAGAAATCCAAAGCTTGTGGTGACTGAGAAGACCATCCGACTTGCTTATCGTCATGCT AAGCAGAATAAAAAAAATTCGTCATGCTTTTTACTTGGTTCTCTGACAGCAGACGAAGATGAAGAAGGT GTAACATTGACAGTAGATCGCTTTGATCCTGGTCGAGAAGTACCTGAATGCCTAGAAATAACCCCTACT GCTTCTCTTCCTGGGGACTTTTTGATTCCATGCAAAGTTCATACTCAAGAACTTTGTTCAAGAGAAATG ATAGTTCACAGTGTAGATGACTTCAGTTCAGCTTTAAAGGCTCTACAGTGCCATATATGTAGCAAAGAT TCCTTGGACTGTGGTAAGCTGCTTTCCCTAAGAGTTCATATCACTTCCAGGGAGAGTTTGGACAGTGTG GAATTTGACTTGCATTGGGCAGCAGTAACTCTAGCAAATAACTTTAAATGCACACCTGTGAAGCCCATC CCCATTATTCCAACAGCTCTGGCAAGAAACTTGAGCAGTAATCTGAATATTTCTCAAGTTCAAGGGACT TATAAATATGGATATCTTACCATGGATGAAACACGCAAATTGTTACTTTTGTTGGAATCTGATCCCAAG GTTTATTCTCTACCATTGGTGGGAATTTGGCTGTCTGGAATTACACATATCTATAGTCCTCAGGTATGG GCTTGCTGTTTGCGATACATATTCAATTCTTCTGTTCAAGAAAGGGTTTTTTCAGAATCTGGAAATTTC ATCATAGTTCTCTATTCTATGACACATAAGGAACCTGAGTTTTATGAATGCTTCCCTTGTGATGGCAAG ATACCTGACTTTCGGTTTCAGTTGCTAACCAGTAAGGAAACATTACATCTTTTCAAAAATGTTGAACCT CCTGACAAAAATCCAATCCGTTGTGAACTGAGCGCTGAAAGCCAAAATGCAGAAACAGAGTTTTTCAGT AAGGCTTCCAAGAATTTTTCAATTAAGAGGTCTTCCCAAAAGTTATCTTCTGGGAAGATGCCAATACAT GATCACGACTCTGGTGTTGAAGATGAAGATTTTTCTCCAAGACCAATTCCTAGTCCTCATCCAGTGAGT CAGAAGATTTCTAAGATCCAACCATCAGTTCCTGAACTTTCACTTGTGTTGGATGGCAATTTCATAGAA TCAAACCCTCTGCCTACTCCATTGGAAATGGTGAATAATGAAAATCCTCCTTTGATTAACCACTTGGAA CACTTGAAGCCATTGCAACCCCAGCTTTATGATGAGAAACACAGTCCAGAAGTTGAAGCTGGAGAGCCT TCCTTGAGAGGAATACCAAATCAGTTAAACCAGGATAAACCAGCTCTTTTGAGACACTGCAAAGTAAGA CAGCCACCTGCCTATAAGAAAGGGAACCCCCATACCAGGAACAGTATTAAACCATCTTCTCATAATGGG CCATCTCATGATATATTTGAAAAGCTCCAAACAGTTTCTGCTGGAAATGTACAAAACGAAGAGTATCCT ATAAGACCCTCCACACTTAATTCTAGGCAGTCTTCTCTTGCCCCGCAGTCCCAACCACACGATTTTGTT TTTTCACCCCATAATTCAGGAAGACCAATGGAACTTCAGATACCTACTCCCCCACTGCCATCTTACTGT TCCACAAACGTTTGCAGGTGTTGTCAGCATCATAGTCATATTCAATATAGTCCGCTAAATTCTTGGCAA GGAGCAAACACAGTTGGATCCATTCAAGATGTCCAGTCTGAAGCCCTTCAAAAGCATTCATTATTTCAC CCAAGTGGATGTCCAGCCCTGTACTGTAATGCATTCTGTTCTTCAAGTAGTCCTATAGCCTTGAGACCT CAGGGAGATATGGGCAGTTGTTCTCCCCACAGCAATATTGAACCATCGCCTGTGGCAAGACCGCCTTCA CATATGGACTTATGTAACCCACAGCCTTGCACAGTGTGCATGCACACACCCAAGACTGAGTCAGATAAT GGAATGATGGGACTATCTCCAGATGCATATCGGTTCCTCACAGAACAAGACAGACAGCTAAGACTACTT CAGGCACAGATTCAGCGTTTGTTGGAAGCACAGTCTCTGATGCCCTGTTCCCCTAAGACAACTGCTGTT GAAGACACAGTGCAAGCTGGAAGACAAATGGAGTTGGTTTCTGTGGAAGCACAGTCTTCCCCTGGCTTG CACATGAGAAAAGGTGTAAGCATTGCTGTGAGCACAGGTGCTAGCTTGTTTTGGAATGCAGCAGGTGAG GATCAAGAGCCTGACTCTCAAATGAAGCAAGATGATACCAAAATTTCCAGTGAGGACATGAATTTTTCT GTCGATATTAATAATGAAGTCACAAGTCTTCCAGGTAGTGCATCTTCATTAAAAGCAGTTGATATTCCC AGTTTTGAAGAGAGCAACATTGCTGTGGAAGAAGAATTTAACCAGCCACTTTCTGTATCCAACTCTTCT CTAGTTGTGAGAAAAGAACCTGATGTACCTGTGTTCTTTCCAAGTGGCCAGCTGGCAGAAAGTGTAAGC ATGTGTTTACAGACTGGACCAACAGGGGGTGCCAGTAACAATTCTGAAACATCAGAGGAACCAAAAATT GAGCATGTAATGCAACCCTTGCTTCATCAACCATCAGATAACCAGAAAATTTACCAGGATTTATTGGGT CAAGTAAACCACCTATTAAATAGTTCCTCCAAGGAAACTGAGCAGCCGTCTACCAAAGCAGTAATTATC AGTCATGAATGCACCAGAACCCAAAACGTTTACCATACAAAGAAAAAAACACATCATTCAAGACTGGTG GACAAAGATTGTGTCCTTAATGCAACTCTTAAGCAACTAAGAAGCCTTGGAGTAAAAATTGATTCTCCC ACTAAAGTGAAGAAAAATGCACATAACGTGGATCACGCCAGTGTGTTGGCATGCATCAGCCCAGAAGCA GTGATCTCTGGATTAAACTGCATGTCATTTGCTAATGTTGGCATGAGCGGCTTAAGCCCCAATGGTGTG GATTTGAGCATGGAGGCAAATGCTATAGCTCTGAAATATTTAAATGAAAATCAGCTGTCACAACTGTCT GTCACTCGATCGAACCAAAATAATTGTGACCCATTCAGCCTTCTCCATATTAATACAGACAGAAGCACA GTGGGGCTTAGTTTAATTTCACCAAACAACATGTCATTTGCAACCAAAAAATATATGAAGAGATATGGA CTCCTACAAAGCAGTGACAATAGTGAAGATGAAGAGGAACCTCCCGACAATGCAGATAGCAAGAGTGAA TATTTATTGAATCAGAACCTTAGGTCCATACCTGAACAGCTTGGTGGTCAGAAAGAGCCTTCTAAGAAT GACCATGAAATAATTAATTGTTCTAACTGTGAATCTGTGGGGACCAACGCAGATACGCCAGTATTGAGA AATATTACAAATGAAGTTTTGCAGACAAAAGCAAAACAGCAGTTGACTGAAAAGCCAGCTTTCTTAGTA AAGAACCTTAAACCAAGTCCTGCAGTGAACCTTCGAACCGGGAAAGCAGAGTTCACTCAACATCCTGAG AAAGAAAATGAAGGGGACATTACAATTTTTCCTGAAAGTTTGCAACCTTCTGAAACGCTAAAGCAGATG AATAGCATGAATTCAGTAGGCACCTTCTTAGATGTAAAACGTCTCAGACAGTTACCAAAATTATTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_003035 |
| Insert Size | 3864 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003035.2 |
| RefSeq Size | 5016 bp |
| RefSeq ORF | 3864 bp |
| Locus ID | 6491 |
| UniProt ID | Q15468 |
| MW | 143 kDa |
| Gene Summary | This gene encodes a cytoplasmic protein implicated in regulation of the mitotic spindle checkpoint, a regulatory pathway that monitors chromosome segregation during cell division to ensure the proper distribution of chromosomes to daughter cells. The protein is phosphorylated in mitosis and in response to activation of the spindle checkpoint, and disappears when cells transition to G1 phase. It interacts with a mitotic regulator, and its expression is required to efficiently activate the spindle checkpoint. It is proposed to regulate Cdc2 kinase activity during spindle checkpoint arrest. Chromosomal deletions that fuse this gene and the adjacent locus commonly occur in T cell leukemias, and are thought to arise through illegitimate V-(D)-J recombination events. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) uses an alternate in-frame splice junction in the 3' coding region compared to variant 1, resulting in an isoform (2) that is 1 aa shorter compared to isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220810 | STIL (Myc-DDK-tagged)-Human SCL/TAL1 interrupting locus (STIL), transcript variant 2 |
CNY 13440.00 |
|
| RC220810L3 | Lenti ORF clone of Human SCL/TAL1 interrupting locus (STIL), transcript variant 2, Myc-DDK-tagged |
CNY 14730.00 |
|
| RC220810L4 | Lenti ORF clone of Human SCL/TAL1 interrupting locus (STIL), transcript variant 2, mGFP tagged |
CNY 14730.00 |
|
| RG220810 | STIL (tGFP-tagged) - Human SCL/TAL1 interrupting locus (STIL), transcript variant 2 |
CNY 15040.00 |
