MSH4 (NM_002440) Human Untagged Clone
CAT＃: SC303201
MSH4 (untagged)-Human mutS homolog 4 (E. coli) (MSH4)
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC303201 representing NM_002440.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCTGAGGCCTGAGATCTCATCAACCTCGCCTTCTGCCCCGGCGGTTTCCCCGTCGTCGGGAGAAACC CGCTCACCTCAGGGTCCCCGCTACAATTTCGGACTCCAGGAGACTCCACAGAGCCGCCCTTCGGTCCAG GTGGTCTCTGCATCCACCTGTCCTGGCACGTCAGGAGCTGCGGGCGACCGGAGCAGCAGCAGCAGCAGC CTTCCCTGCCCCGCGCCAAACTCCCGGCCAGCTCAAGGTTCATACTTTGGAAACAAAAGAGCTTATGCA GAAAACACAGTTGCATCAAATTTTACTTTTGGTGCAAGCTCATCTTCTGCACGAGATACTAATTATCCT CAAACACTTAAAACTCCATTGTCTACTGGAAATCCTCAGAGATCAGGTTATAAGAGCTGGACACCACAA GTGGGATATTCAGCTTCATCCTCATCTGCGATTTCTGCACACTCCCCATCAGTTATTGTAGCTGTTGTA GAAGGGAGAGGACTTGCCAGAGGTGAAATAGGAATGGCAAGTATTGATTTAAAAAACCCCCAAATTATA CTATCCCAGTTTGCAGACAACACAACATATGCAAAGGTGATCACTAAACTTAAAATTTTATCACCTTTG GAAATAATAATGTCAAATACTGCTTGTGCTGTGGGGAATTCCACCAAGTTGTTCACTCTGATCACAGAA AATTTCAAGAATGTTAATTTCACTACTATCCAAAGGAAATACTTCAATGAAACAAAAGGATTAGAGTAC ATTGAACAGTTATGCATAGCAGAATTCAGCACTGTCCTAATGGAGGTTCAGTCCAAGTATTACTGCCTT GCAGCTGTTGCAGCTTTGTTAAAATATGTTGAATTTATTCAAAATTCAGTTTATGCACCAAAATCACTG AAGATTTGTTTCCAGGGTAGTGAACAGACAGCCATGATAGATTCATCATCAGCCCAAAACCTTGAATTG TTAATTAATAATCAAGACTATAGGAATAATCACACTCTCTTTGGTGTTCTAAATTATACTAAGACTCCT GGAGGGAGTAGACGACTTCGTTCTAATATATTAGAGCCTCTAGTTGATATTGAAACCATTAACATGAGA TTAGATTGTGTTCAAGAACTACTTCAAGATGAGGAACTATTTTTTGGACTTCAATCAGTTATATCAAGA TTTCTTGATACAGAGCAGCTTCTTTCTGTTTTAGTCCAAATTCCAAAGCAAGACACGGTCAATGCTGCT GAATCAAAGATAACAAATTTAATATACTTAAAACATACCTTGGAACTTGTGGATCCTTTAAAGATTGCT ATGAAGAACTGTAACACACCTTTATTAAGAGCTTACTATGGTTCCTTGGAAGACAAGAGGTTTGGAATC ATACTTGAAAAGATTAAAACAGTAATTAATGATGATGCAAGATACATGAAAGGATGCCTAAACATGAGG ACTCAGAAGTGCTATGCAGTGAGGTCTAACATAAATGAATTTCTTGACATAGCAAGAAGAACATACACA GAGATTGTAGATGACATAGCAGGAATGATATCACAACTTGGAGAAAAATATAGTCTACCTTTAAGGACA AGTTTTAGCTCTGCTCGAGGATTTTTCATCCAGATGACTACAGATTGTATAGCCCTACCTAGTGATCAA CTTCCTTCAGAATTTATTAAGATTTCTAAAGTGAAAAATTCTTACAGCTTTACATCAGCAGATTTAATT AAAATGAATGAAAGATGCCAAGAATCTTTGAGAGAAATCTATCACATGACTTATATGATAGTGTGCAAA CTGCTTAGTGAGATTTATGAACATATTCATTGCTTATATAAACTATCTGACACTGTGTCAATGCTGGAT ATGCTACTGTCATTTGCTCATGCCTGCACTCTTTCTGACTATGTTCGACCAGAATTTACTGATACTTTA GCAATCAAACAGGGATGGCATCCTATTCTTGAAAAAATATCTGCGGAAAAACCTATTGCCAACAATACC TATGTTACAGAAGGGAGTAATTTTTTGATCATAACTGGACCAAACATGAGTGGAAAATCCACATATTTA AAACAGATTGCTCTTTGTCAGATTATGGCCCAGATTGGATCATATGTTCCAGCAGAATATTCTTCCTTT AGAATTGCTAAACAGATTTTTACAAGAATTAGTACTGATGATGATATCGAAACAAATTCATCAACATTT ATGAAAGAAATGAAAGAGATAGCATATATTCTACATAATGCTAATGACAAATCGCTCATATTAATTGAT GAACTTGGCAGAGGTACTAATACGGAAGAAGGTATTGGCATTTGTTATGCTGTTTGTGAATATCTACTG AGCTTAAAGGCATTTACACTGTTTGCTACACATTTCCTGGAACTATGCCATATTGATGCCCTGTATCCT AATGTAGAAAACATGCATTTTGAAGTTCAACATGTAAAGAATACCTCAAGAAATAAAGAAGCAATTTTG TATACCTACAAACTTTCTAAGGGACTCACAGAAGAGAAAAATTATGGATTAAAAGCTGCAGAGGTGTCA TCACTTCCACCATCAATTGTCTTGGATGCCAAGGAAATCACAACTCAAATTACGAGACAAATTTTGCAA AACCAAAGGAGTACCCCTGAGATGGAAAGACAGAGAGCTGTGTACCATCTAGCCACTAGGCTTGTTCAA ACTGCTCGAAACTCTCAATTGGATCCAGACAGTTTACGAATATATTTAAGTAACCTCAAGAAGAAGTAC AAAGAAGATTTTCCCAGGACTGAACAAGTTCCAGAAAAGACTGAAGAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_002440 |
| Insert Size | 2811 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002440.3 |
| RefSeq Size | 3293 bp |
| RefSeq ORF | 2811 bp |
| Locus ID | 4438 |
| UniProt ID | O15457 |
| Protein Families | Druggable Genome |
| MW | 104.8 kDa |
| Gene Summary | This gene encodes a member of the DNA mismatch repair mutS family. This member is a meiosis-specific protein that is not involved in DNA mismatch correction, but is required for reciprocal recombination and proper segregation of homologous chromosomes at meiosis I. This protein and MSH5 form a heterodimer which binds uniquely to a Holliday Junction and its developmental progenitor, thus provoking ADP-ATP exchange, and stabilizing the interaction between parental chromosomes during meiosis double-stranded break repair. [provided by RefSeq, Aug 2011] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206059 | MSH4 (Myc-DDK-tagged)-Human mutS homolog 4 (E. coli) (MSH4) |
CNY 6856.00 |
|
| RC206059L3 | Lenti ORF clone of Human mutS homolog 4 (E. coli) (MSH4), Myc-DDK-tagged |
CNY 9030.00 |
|
| RC206059L4 | Lenti ORF clone of Human mutS homolog 4 (E. coli) (MSH4), mGFP tagged |
CNY 9030.00 |
|
| RG206059 | MSH4 (tGFP-tagged) - Human mutS homolog 4 (E. coli) (MSH4) |
CNY 8456.00 |
