LCT (NM_002299) Human Untagged Clone
CNY 37050.00
货期*
4周
规格
Product images
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Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | LAC; LPH; LPH1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC303171 representing NM_002299.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGCTGTCTTGGCATGTAGTCTTTATTGCCCTGCTAAGTTTTTCATGCTGGGGGTCAGACTGGGAG TCTGATAGAAATTTCATTTCCACCGCTGGTCCTCTAACCAATGACTTGCTGCACAACCTGAGTGGTCTC CTGGGAGACCAGAGTTCTAACTTTGTAGCAGGGGACAAAGACATGTATGTTTGTCACCAGCCACTGCCC ACTTTCCTGCCAGAATACTTCAGCAGTCTCCATGCCAGTCAGATCACCCATTATAAGGTATTTCTGTCA TGGGCACAGCTCCTCCCAGCAGGAAGCACCCAGAATCCAGACGAGAAAACAGTGCAGTGCTACCGGCGA CTCCTCAAGGCCCTCAAGACTGCACGGCTTCAGCCCATGGTCATCCTGCACCACCAGACCCTCCCTGCC AGCACCCTCCGGAGAACCGAAGCCTTTGCTGACCTCTTCGCCGACTATGCCACATTCGCCTTCCACTCC TTCGGGGACCTAGTTGGGATCTGGTTCACCTTCAGTGACTTGGAGGAAGTGATCAAGGAGCTTCCCCAC CAGGAATCAAGAGCGTCACAACTCCAGACCCTCAGTGATGCCCACAGAAAAGCCTATGAGATTTACCAC GAAAGCTATGCTTTTCAGGGCGGAAAACTCTCTGTTGTCCTGCGAGCTGAAGATATCCCGGAGCTCCTG CTAGAACCACCCATATCTGCGCTTGCCCAGGACACGGTCGATTTCCTCTCTCTTGATTTGTCTTATGAA TGCCAAAATGAGGCAAGTCTGCGGCAGAAGCTGAGTAAATTGCAGACCATTGAGCCAAAAGTGAAAGTT TTCATCTTCAACCTAAAACTCCCAGACTGCCCCTCCACCATGAAGAACCCAGCCAGTCTGCTCTTCAGC CTTTTTGAAGCCATAAATAAAGACCAAGTGCTCACCATTGGGTTTGATATTAATGAGTTTCTGAGTTGT TCATCAAGTTCCAAGAAAAGCATGTCTTGTTCTCTGACTGGCAGCCTGGCCCTTCAGCCTGACCAGCAG CAGGACCACGAGACCACGGACTCCTCTCCTGCCTCTGCCTATCAGAGAATCTGGGAAGCATTTGCCAAT CAGTCCAGGGCGGAAAGGGATGCCTTCCTGCAGGATACTTTCCCTGAAGGCTTCCTCTGGGGTGCCTCC ACAGGAGCCTTTAACGTGGAAGGAGGCTGGGCCGAGGGTGGGAGAGGGGTGAGCATCTGGGATCCACGC AGGCCCCTGAACACCACTGAGGGCCAAGCGACGCTGGAGGTGGCCAGCGACAGTTACCACAAGGTAGCC TCTGACGTCGCCCTGCTTTGCGGCCTCCGGGCTCAGGTGTACAAGTTCTCCATCTCCTGGTCCCGGATC TTCCCCATGGGGCACGGGAGCAGCCCCAGCCTCCCAGGCGTTGCCTACTACAACAAGCTGATTGACAGG CTACAGGATGCGGGCATCGAGCCCATGGCCACGCTGTTCCACTGGGACCTGCCTCAGGCCCTGCAGGAT CATGGTGGATGGCAGAATGAGAGCGTGGTGGATGCCTTCCTGGACTATGCGGCCTTCTGCTTCTCCACA TTTGGGGACCGTGTGAAGCTGTGGGTGACCTTCCATGAGCCGTGGGTGATGAGCTACGCAGGCTATGGC ACCGGCCAGCACCCTCCCGGCATCTCTGACCCAGGAGTGGCCTCTTTTAAGGTGGCTCACTTGGTCCTC AAGGCTCATGCCAGAACTTGGCACCACTACAACAGCCATCATCGCCCACAGCAGCAGGGGCACGTGGGC ATTGTGCTGAACTCAGACTGGGCAGAACCCCTGTCTCCAGAGAGGCCTGAGGACCTGAGAGCCTCTGAG CGCTTCTTGCACTTCATGCTGGGCTGGTTTGCACACCCCGTCTTTGTGGATGGAGACTACCCAGCCACC CTGAGGACCCAGATCCAACAGATGAACAGACAGTGCTCCCATCCTGTGGCTCAACTCCCCGAGTTCACA GAGGCAGAGAAGCAGCTCCTGAAAGGCTCTGCTGATTTTCTGGGTCTGTCGCATTACACCTCCCGCCTC ATCAGCAACGCCCCACAAAACACCTGCATCCCTAGCTATGATACCATTGGAGGCTTCTCCCAACACGTG AACCATGTGTGGCCCCAGACCTCATCCTCTTGGATTCGTGTGGTGCCCTGGGGGATAAGGAGGCTGTTG CAGTTTGTATCCCTGGAATACACAAGAGGAAAAGTTCCAATATACCTTGCCGGGAATGGCATGCCCATA GGGGAAAGTGAAAATCTCTTTGATGATTCCTTAAGAGTAGACTACTTCAATCAATATATCAATGAGGTG CTCAAGGCTATCAAGGAAGACTCTGTGGATGTTCGTTCCTACATTGCTCGTTCCCTCATTGATGGCTTC GAAGGCCCTTCTGGTTACAGCCAGCGGTTTGGCCTGCACCACGTCAACTTCAGCGACAGCAGCAAGTCA AGGACTCCCAGGAAATCTGCCTACTTTTTCACTAGCATCATAGAAAAGAACGGTTTCCTCACCAAGGGG GCAAAAAGACTGCTACCACCTAATACAGTAAACCTCCCCTCCAAAGTCAGAGCCTTCACTTTTCCATCT GAGGTGCCCTCCAAGGCTAAAGTCGTTTGGGAAAAGTTCTCCAGCCAACCCAAGTTCGAAAGAGATTTG TTCTACCACGGGACGTTTCGGGATGACTTTCTGTGGGGCGTGTCCTCTTCCGCTTATCAGATTGAAGGC GCGTGGGATGCCGATGGCAAAGGCCCCAGCATCTGGGATAACTTTACCCACACACCAGGGAGCAATGTG AAAGACAATGCCACTGGAGACATCGCCTGTGACAGCTATCACCAGCTGGATGCCGATCTGAATATGCTC CGAGCTTTGAAGGTGAAGGCCTACCGCTTCTCTATCTCCTGGTCTCGGATTTTCCCAACTGGGAGAAAC AGCTCTATCAACAGTCATGGGGTTGATTATTACAACAGGCTGATCAATGGCTTGGTGGCAAGCAACATC TTTCCCATGGTGACATTGTTCCATTGGGACCTGCCCCAGGCCCTCCAGGATATCGGAGGCTGGGAGAAT CCTGCCTTGATTGACTTGTTTGACAGCTACGCAGACTTTTGTTTCCAGACCTTTGGTGATAGAGTCAAG TTTTGGATGACTTTTAATGAGCCCATGTACCTGGCATGGCTAGGTTATGGCTCAGGGGAATTTCCCCCA GGGGTGAAGGACCCAGGCTGGGCACCATATAGGATAGCCCACGCCGTCATCAAAGCCCATGCCAGAGTC TATCACACGTACGATGAGAAATACAGGCAGGAGCAGAAGGGGGTCATCTCGCTGAGCCTCAGTACACAC TGGGCAGAGCCCAAGTCACCAGGGGTCCCCAGAGATGTGGAAGCCGCTGACCGAATGCTGCAGTTCTCC CTGGGCTGGTTTGCTCACCCCATTTTTAGAAACGGAGACTATCCTGACACCATGAAGTGGAAAGTGGGG AACAGGAGTGAACTGCAGCACTTAGCCACCTCCCGCCTGCCAAGCTTCACTGAGGAAGAGAAGAGGTTC ATCAGGGCGACGGCCGACGTCTTCTGCCTCAACACGTACTACTCCAGAATCGTGCAGCACAAAACACCC AGGCTAAACCCACCCTCCTACGAAGACGACCAGGAGATGGCTGAGGAGGAGGACCCTTCGTGGCCTTCC ACGGCAATGAACAGAGCTGCGCCCTGGGGGACGCGAAGGCTGCTGAACTGGATCAAGGAAGAGTATGGT GACATCCCCATTTACATCACCGAAAACGGAGTGGGGCTGACCAATCCGAACACGGAGGATACTGATAGG ATATTTTACCACAAAACCTACATCAATGAGGCTTTGAAAGCCTACAGGCTCGATGGTATAGACCTTCGA GGGTATGTCGCCTGGTCTCTGATGGACAACTTTGAGTGGCTAAATGGCTACACGGTCAAGTTTGGACTG TACCATGTTGATTTCAACAACACGAACAGGCCTCGCACAGCAAGAGCCTCCGCCAGGTACTACACAGAG GTCATTACCAACAACGGCATGCCACTGGCCAGGGAGGATGAGTTTCTGTACGGACGGTTTCCTGAGGGC TTCATCTGGAGTGCAGCTTCTGCTGCATATCAGATTGAAGGTGCGTGGAGAGCAGATGGCAAAGGACTC AGCATTTGGGACACGTTTTCTCACACACCACTGAGGGTTGAGAACGATGCCATTGGAGACGTGGCCTGT GACAGTTATCACAAGATTGCTGAGGATCTGGTCACCCTGCAGAACCTGGGCGTGTCCCACTACCGTTTT TCCATCTCCTGGTCTCGCATCCTCCCTGATGGAACCACCAGGTACATCAATGAAGCGGGCCTGAACTAC TACGTGAGGCTCATCGATACACTGCTGGCCGCCAGCATCCAGCCCCAGGTGACCATTTACCACTGGGAC CTACCACAGACGCTCCAAGATGTAGGAGGCTGGGAGAATGAGACCATCGTGCAGCGGTTTAAGGAGTAT GCAGATGTGCTCTTCCAGAGGCTGGGAGACAAGGTGAAGTTTTGGATCACGCTGAATGAGCCCTTTGTC ATTGCTTACCAGGGCTATGGCTACGGAACAGCAGCTCCAGGAGTCTCCAATAGGCCTGGCACTGCCCCC TACATTGTTGGCCACAATCTAATAAAGGCTCATGCTGAGGCCTGGCATCTGTACAACGATGTGTACCGC GCCAGTCAAGGTGGCGTGATTTCCATCACCATCAGCAGTGACTGGGCTGAACCCAGAGATCCCTCTAAC CAGGAGGATGTGGAGGCAGCCAGGAGATATGTTCAGTTCATGGGAGGCTGGTTTGCACATCCTATTTTC AAGAATGGAGATTACAATGAGGTGATGAAGACGCGGATCCGTGACAGGAGCTTGGCTGCAGGCCTCAAC AAGTCTCGGCTGCCAGAATTTACAGAGAGTGAGAAGAGGAGGATCAACGGCACCTATGACTTTTTTGGG TTCAATCACTACACCACTGTCCTCGCCTACAACCTCAACTATGCCACTGCCATCTCTTCTTTTGATGCA GACAGAGGAGTTGCTTCCATCGCAGATCGCTCGTGGCCAGACTCTGGCTCCTTCTGGCTGAAGATGACG CCTTTTGGCTTCAGGAGGATCCTGAACTGGTTAAAGGAGGAATACAATGACCCTCCAATTTATGTCACA GAGAATGGAGTGTCCCAGCGGGAAGAAACAGACCTCAATGACACTGCAAGGATCTACTACCTTCGGACT TACATCAATGAGGCCCTCAAAGCTGTGCAGGACAAGGTGGACCTTCGAGGATACACAGTTTGGAGTGCG ATGGACAATTTTGAGTGGGCCACAGGCTTTTCAGAGAGATTTGGTCTGCATTTTGTGAACTACAGTGAC CCTTCTCTGCCAAGGATCCCCAAAGCATCAGCGAAGTTCTACGCCTCTGTGGTCCGATGCAATGGCTTC CCTGACCCCGCTACAGGGCCTCACGCTTGTCTCCACCAGCCAGATGCTGGACCCACCATCAGCCCCGTG AGACAGGAGGAGGTGCAGTTCCTGGGGCTAATGCTCGGCACCACAGAAGCACAGACAGCTTTGTACGTT CTCTTTTCTCTTGTGCTTCTTGGAGTCTGTGGCTTGGCATTTCTGTCATACAAGTACTGCAAGCGCTCT AAGCAAGGGAAAACACAACGAAGCCAACAGGAATTGAGCCCGGTGTCTTCATTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_002299 |
| Insert Size | 5784 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002299.3 |
| RefSeq Size | 6288 bp |
| RefSeq ORF | 5784 bp |
| Locus ID | 3938 |
| UniProt ID | P09848 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Galactose metabolism |
| MW | 218.6 kDa |
| Gene Summary | The protein encoded by this gene belongs to the glycosyl hydrolase 1 family of proteins. The encoded preproprotein is proteolytically processed to generate the mature enzyme. This enzyme is integral to the plasma membrane and has both phlorizin hydrolase activity and lactase activity. Mutations in this gene are associated with congenital lactase deficiency. Polymorphisms in this gene are associated with lactase persistence, in which intestinal lactase activity persists at childhood levels into adulthood. [provided by RefSeq, Jan 2016] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212751 | LCT (Myc-DDK-tagged)-Human lactase (LCT) |
CNY 12952.00 |
|
| RC212751L1 | Lenti ORF clone of Human lactase (LCT), Myc-DDK-tagged |
CNY 15352.00 |
|
| RG212751 | LCT (tGFP-tagged) - Human lactase (LCT) |
CNY 15200.00 |
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