KRT85 (NM_002283) Human Untagged Clone
CAT＃: SC303167
KRT85 (untagged)-Human keratin 85 (KRT85)
CNY 8080.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ECTD4; Hb-5; HB5; hHb5; K85; KRTHB5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC303167 representing NM_002283.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCGTGCCGCTCCTACAGGATCAGCTCAGGATGCGGGGTCACCAGGAACTTCAGCTCCTGCTCAGCT GTGGCCCCCAAAACTGGCAACCGCTGCTGCATCAGCGCCGCCCCCTACCGAGGGGTGTCCTGCTACCGA GGGCTGACGGGCTTCGGCAGCCGCAGCCTCTGCAACCTGGGCTCCTGCGGGCCCCGGATAGCTGTAGGT GGCTTCCGAGCCGGCTCCTGCGGACGCAGCTTCGGCTACCGCTCCGGGGGCGTGTGCGGACCCAGCCCC CCATGCATCACTACCGTGTCGGTCAACGAGAGCCTCCTCACGCCCCTCAACCTGGAGATCGACCCCAAC GCACAGTGCGTGAAGCAGGAGGAGAAGGAGCAGATCAAGTCCCTCAACAGCAGGTTCGCGGCCTTCATC GACAAGGTGCGCTTCCTGGAGCAGCAGAACAAGCTGCTGGAGACCAAGTGGCAGTTCTACCAGAACCAG CGCTGCTGCGAGAGCAACCTGGAGCCACTGTTCAGTGGCTACATCGAGACTCTGCGGCGGGAGGCCGAG TGCGTGGAGGCCGACAGCGGGAGGCTGGCCTCAGAGCTCAACCATGTGCAGGAGGTGCTGGAGGGCTAC AAGAAGAAGTATGAAGAGGAGGTGGCCCTGAGAGCCACAGCAGAGAATGAGTTTGTCGTTCTAAAGAAG GACGTGGACTGTGCCTACCTGCGGAAATCAGACCTGGAGGCCAATGTGGAGGCCCTGGTGGAGGAGTCT AGCTTCCTGAGGCGCCTCTATGAAGAGGAGATCCGCGTTCTCCAAGCCCACATCTCAGACACCTCGGTC ATAGTCAAGATGGACAACAGCCGAGACCTGAACATGGACTGCATCATCGCTGAGATCAAGGCTCAGTAT GACGATGTTGCCAGCCGCAGCCGGGCCGAGGCTGAGTCCTGGTACCGTAGCAAGTGTGAGGAGATGAAG GCCACGGTGATCAGGCATGGGGAGACCCTGCGCCGCACCAAGGAGGAGATCAACGAGCTGAACCGCATG ATCCAGAGGCTGACGGCCGAGATTGAGAATGCCAAGTGCCAGCGTGCCAAGCTGGAGGCTGCTGTGGCT GAGGCAGAGCAGCAGGGTGAGGCGGCCCTCAGCGATGCCCGCTGCAAGCTGGCTGAGCTGGAGGGCGCC CTGCAGAAGGCCAAGCAGGACATGGCCTGCCTGCTCAAGGAGTACCAGGAGGTGATGAACTCCAAGCTG GGCCTGGACATCGAGATCGCCACCTACAGGCGCCTGCTGGAGGGCGAGGAACACAGGCTGTGTGAAGGT GTGGGCTCTGTGAATGTCTGTGTCAGCAGCTCCCGTGGTGGAGTCTCCTGTGGGGGCCTCTCCTACAGC ACCACCCCAGGGCGCCAGATCACTTCTGGCCCCTCAGCCATAGGCGGCAGCATCACGGTGGTGGCCCCT GACTCCTGTGCCCCCTGCCAGCCTCGTTCCTCCAGCTTCAGCTGCGGGAGTAGCCGGTCGGTCCGCTTT GCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_002283 |
| Insert Size | 1524 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002283.3 |
| RefSeq Size | 2491 bp |
| RefSeq ORF | 1524 bp |
| Locus ID | 3891 |
| UniProt ID | P78386 |
| MW | 55.8 kDa |
| Gene Summary | The protein encoded by this gene is a member of the keratin gene family. As a type II hair keratin, it is a basic protein which heterodimerizes with type I keratins to form hair and nails. The type II hair keratins are clustered in a region of chromosome 12q13 and are grouped into two distinct subfamilies based on structure similarity. One subfamily, consisting of KRTHB1, KRTHB3, and KRTHB6, is highly related. The other less-related subfamily includes KRTHB2, KRTHB4, and KRTHB5. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220351 | KRT85 (Myc-DDK-tagged)-Human keratin 85 (KRT85) |
CNY 3776.00 |
|
| RC220351L3 | Lenti ORF clone of Human keratin 85 (KRT85), Myc-DDK-tagged |
CNY 6460.00 |
|
| RC220351L4 | Lenti ORF clone of Human keratin 85 (KRT85), mGFP tagged |
CNY 6460.00 |
|
| RG220351 | KRT85 (tGFP-tagged) - Human keratin 85 (KRT85) |
CNY 5040.00 |
