RSPH4A (NM_001010892) Human Untagged Clone
CAT＃: SC301511
RSPH4A (untagged)-Human radial spoke head 4 homolog A (Chlamydomonas) (RSPH4A), transcript variant 1
CNY 13780.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CILD11; dJ412I7.1; RSHL3; RSPH6B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC301511 representing NM_001010892.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGGACTCAACCTCCCCGAAGCAAGAAAAAGAAAACCAAGAAGAACTAGGGGAAACAAGGCGGCCA TGGGAAGGAAAGACAGCAGCTTCTCCCCAATATTCTGAGCCTGAGTCGTCTGAGCCCTTGGAGGCGAAG CAGGGGCCAGAAACTGGACGCCAGTCCCGAAGCAGCCGTCCTTGGAGCCCGCAGTCTAGAGCCAAGACG CCTCTGGGTGGCCCCGCGGGACCAGAAACATCATCACCTGCTCCTGTCTCTCCGCGGGAGCCCTCTTCC TCTCCTTCTCCCCTGGCTCCGGCCAGACAAGACCTCGCGGCACCACCTCAGTCGGACAGGACCACGAGT GTGATTCCTGAAGCTGGGACACCTTATCCTGATCCTTTGGAACAATCATCTGATAAAAGAGAATCAACT CCTCATCACACAAGCCAGTCAGAAGGAAACACCTTTCAACAGTCTCAGCAACCCAAACCCCACCTGTGT GGACGAAGGGACGTGAGCTATAACAACGCTAAACAGAAAGAGCTGAGATTTGACGTTTTTCAGGAGGAA GACTCAAACAGTGACTATGATTTACAGCAGCCGGCGCCTGGGGGCTCTGAAGTGGCCCCCAGCATGCTT GAGATCACCATTCAGAATGCTAAGGCTTACCTGCTGAAGACTAGCAGCAATTCGGGCTTTAATCTATAT GATCATCTTTCTAATATGTTGACCAAGATATTAAATGAGCGTCCTGAAAATGCTGTTGACATCTTTGAA AATATTAGCCAAGATGTGAAGATGGCACATTTTAGTAAAAAATTTGATGCACTACAAAATGAGAATGAG TTGCTTCCAACATATGAAATAGCAGAAAAGCAAAAGGCTCTTTTTCTCCAGGGACATTTGGAAGGAGTT GACCAAGAATTGGAAGATGAAATAGCAGAAAACGCTCTTCCAAATGTAATGGAGTCAGCTTTTTATTTT GAACAAGCTGGAGTTGGTTTGGGCACAGATGAGACATACCGCATATTTCTTGCCCTCAAGCAGCTTACT GATACCCACCCAATCCAAAGATGCCGCTTCTGGGGAAAGATCTTGGGTCTGGAAATGAATTATATTGTA GCTGAAGTGGAATTTCGTGAGGGGGAAGATGAAGAGGAAGTGGAAGAGGAAGATGTAGCTGAAGAGAGG GACAATGGAGAAAGTGAAGCTCATGAAGATGAGGAAGATGAATTACCAAAGTCCTTTTACAAGGCCCCA CAGGCTATACCAAAAGAAGAAAGTAGAACAGGTGCCAACAAATATGTCTATTTTGTTTGCAATGAACCA GGAAGACCATGGGTGAAGTTACCACCAGTTATACCTGCACAAATTGTTATTGCAAGAAAAATCAAGAAA TTTTTCACTGGGCGATTGGATGCTCCCATCATAAGCTACCCACCTTTCCCAGGAAATGAGAGTAATTAT TTACGAGCACAAATTGCCCGAATTTCAGCAGGAACCCACGTCAGTCCTCTAGGATTTTATCAGTTTGGT GAAGAGGAAGGAGAGGAGGAGGAAGAGGCAGAAGGTGGGCGAAATAGCTTTGAGGAAAACCCTGATTTT GAAGGCATCCAAGTGATTGATCTAGTAGAATCCCTATCCAATTGGGTTCATCATGTACAGCATATTCTC TCTCAGGGTCGCTGTAATTGGTTCAACTCCATACAAAAAAATGAGGAAGAAGAAGAGGAAGAAGATGAA GAAAAAGACGATTCTGACTACATAGAACAGGAAGTGGGGCTTCCTCTTTTGACACCAATCTCTGAAGAT TTAGAGATTCAGAATATACCCCCCTGGACAACACGGTTATCCTCAAATCTCATTCCACAATATGCTATT GCAGTCCTTCAATCCAACCTTTGGCCTGGAGCATATGCCTTCTCCAATGGCAAAAAGTTTGAAAATTTC TACATAGGCTGGGGTCATAAGTATAGTCCAGACAATTATACACCCCCAGTTCCACCACCAGTTTATCAA GAATACCCCAGTGGACCAGAAATTACAGAAATGGATGATCCTAGTGTGGAGGAGGAGCAGGCTTTCAGG GCTGCACAAGAAGCAGTTCTACTCGCAGCTGAGAATGAAGAATCTGAGGAAGATGAAGATGAGGAAGAT GATTATGACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001010892 |
| Insert Size | 2151 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001010892.2 |
| RefSeq Size | 2858 bp |
| RefSeq ORF | 2151 bp |
| Locus ID | 345895 |
| UniProt ID | Q5TD94 |
| MW | 80.7 kDa |
| Gene Summary | This gene encodes a protein that appears to be a component the radial spoke head, as determined by homology to similar proteins in the biflagellate alga Chlamydomonas reinhardtii and other ciliates. Radial spokes, which are regularly spaced along cilia, sperm, and flagella axonemes, consist of a thin 'stalk' and a bulbous 'head' that form a signal transduction scaffold between the central pair of microtubules and dynein. Mutations in this gene cause primary ciliary dyskinesia 1, a disease arising from dysmotility of motile cilia and sperm. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009] Transcript Variant: This variant (1) encodes the longer isoform (1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223494 | RSPH4A (Myc-DDK-tagged)-Human radial spoke head 4 homolog A (Chlamydomonas) (RSPH4A), transcript variant 1 |
CNY 5248.00 |
|
| RC223494L3 | Lenti ORF clone of Human radial spoke head 4 homolog A (Chlamydomonas) (RSPH4A), transcript variant 1, Myc-DDK-tagged |
CNY 7700.00 |
|
| RC223494L4 | Lenti ORF clone of Human radial spoke head 4 homolog A (Chlamydomonas) (RSPH4A), transcript variant 1, mGFP tagged |
CNY 7700.00 |
|
| RG223494 | RSPH4A (tGFP-tagged) - Human radial spoke head 4 homolog A (Chlamydomonas) (RSPH4A), transcript variant 1 |
CNY 6370.00 |
