TDRD6 (NM_001010870) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	bA446F17.4; CT41.2; NY-CO-45; SPATA36; TDR2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC301493 representing NM_001010870. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTGCTCGACGCCCGGAATGCCGGCGCCGGGGGCCTCGCTGGCCCTGCGGGTGTCCTTCGTGGACGTG CATCCCGATGTGATCCCGGTGCAGCTGTGGGGGCTGGTGGGCGAGCGGCGGGGCGAGTACCTGCGGCTG AGCCGGGAAATCCAGGAAGCGGCGGCCACGCGCGGCCAGTGGGCGCTGGGCAGCGCCTCGGCCTCGCCC GGCGAGCTGTGCCTGGTGCAGGTCGGGCTTTTGTGGCACCGCTGCCGCGTGGTCAGCCGGCAGGCACAG GAGAGCCGTGTCTTCCTGCTGGACGAGGGCCGCACCATCACGGCCGGAGCAGGCTCGCTGGCGCCTGGG CGCAGAGAGTTCTTCAATTTGCCCTCGGAAGTGCTGGGCTGCGTGCTAGCGGGCCTGGTGCCGGCAGGC TGCGGCGCGGGCTCAGGCGAGCCGCCGCAGCACTGGCCCGCCGACGCCGTGGACTTCCTTAGCAACCTT CAGGGCAAGGAGGTGCACGGGTGCGTCCTGGACGTGCTGCTGCTCCATCGCCTGGTCCTCCTGGAGGTG CCTGATGTGTTCCAACAGATGCGGGAGCTGGGCCTGGCTCGGCGGGTGCCCGACAGCCTCTTCCGTTCG CTGCTGGAGCGCTATCTCACAGCGGCCACTGCTAGCGTGGGCTCCGGGGTCCCGGTTCTCTCGCGAGTC CCGCTCAAGCAAAAGCAGCCTGGTCTGGATTACTTCTATCCCCAGCTGCAGCTGGGCGTGACGGAGGCC GTGGTCATAACCCAAGTGTGCCATCCCCACCGCATTCACTGCCAGCTCCGCAGCGTCTCGCAGGAGATC CACCGCCTCTCCGAGAGCATGGCCCAGGTATACCGGGGTTCCACGGGGACAGGGGATGAGAACTCTACC AGTGCCACCTGGGAGGAGAGGGAGGAGAGCCCAGATAAGCCGGGCTCTCCGTGTGCATCCTGTGGCCTG GATGGACATTGGTACAGAGCACTGTTGCTTGAGACTTTTCGGCCCCAGCGCTGTGCCCAGGTGCTTCAT GTGGACTATGGAAGGAAGGAGTTAGTGAGTTGCAGCAGCCTTCGGTACTTGCTGCCTGAATATTTTCGA ATGCCGGTGGTGACCTACCCTTGTGCTTTGTATGGACTCTGGGACGGTGGGAGAGGCTGGTCTCGGTCA CAGGTCGGTGACCTGAAGACACTGATACTAGGCAAGGCAGTGAATGCAAAGATTGAATTTTATTGCTCC TTTGAGCATGTGTATTATGTCAGCCTGTATGGAGAAGATGGGATTAATCTGAACCGTGTGTTTGGAGTA CAGTCGTGTTGCTTGGCTGACCGAGTCCTTCAGAGCCAGGCAACAGAGGAGGAGGAACCAGAAACATCT CAGTCTCAGTCTCCTGCTGAAGAAGTAGATGAAGAGATTTCACTCCCAGCCTTAAGATCTATCAGGTTA AAGATGAATGCCTTCTACGATGCGCAGGTAGAGTTTGTTAAAAATCCTTCTGAGTTTTGGATTAGGTTG AGGAAACACAATGTCACCTTCAGTAAGCTGATGAGGAGAATGTGTGGTTTCTATTCCTCTGCCAGTAAG CTGGATGGTGTAGTTTTGAAACCTGAACCTGATGACCTTTGCTGTGTCAAGTGGAAAGAAAATGGTTAT TATAGGGCCATAGTCACCAAATTGGATGACAAGAGTGTGGATGTATTCTTAGTTGACCGAGGCAATTCG GAAAATGTGGACTGGTATGACGTAAGGATGCTGCTTCCTCAGTTTAGGCAGCTACCAATATTGGCTGTG AAGTGCACCCTGGCTGATATTTGGCCTTTGGGAAAAACTTGGAGCCAGGAGGCAGTTTCCTTTTTTAAA AAGACTGTGCTCCACAAAGAATTAGTCATCCATATTCTTGATAAACAGGATCATCAATATGTTATTGAG ATTCTTGACGAATCAAGAACAGGGGAAGAAAACATTAGTAAGGTAATTGCCCAAGCTGGATATGCCAAG TATCAGGAATTTGAAACAAAGGAAAATATCCTGGTAAATGCCCACTCCCCAGGGCATGTTTCAAACCAC TTTACTACGGAGAGTAACAAAATACCTTTTGCCAAGACTGGAGAAGGAGAGCAGAAAGCCAAGAGAGAG AATAAAACCACATCTGTTTCAAAAGCTTTGAGTGACACAACAGTTGTAACAAATGGTTCAACTGAACTA GTTGTGCAGGAAAAAGTGAAAAGAGCATCTGTTTATTTTCCTCTTATGCAGAATTGCTTGGAAATTAAG CCAGGCTCCTCTAGTAAAGGAGAGCTGGAAGTTGGAAGTACAGTAGAAGTCAGAGTGTCTTATGTTGAA AACCCTGGCTATTTCTGGTGTCAGCTGACCAGGAACATACAAGGACTTAAAACTCTAATGTCTGATATT CAGTACTATTGCAAAAATACAGCTGCTCCTCACCAGAGAAACACCCTTGCTTGTTTGGCTAAGCGAACA GTAAACAGACAGTGGTCCAGAGCACTTATTAGTGGGATACAGTCTGTGGAGCATGTCAATGTAACATTT GTAGATTATGGAGACAGAGAAATGGTATCTGTGAAGAATATTTATTCAATTAGTGAAGAATTTCTGAAG GTTAAGGCACAGGCTTTTAGGTGCAGTCTTTATAATTTAATTCAACCAGTTGGCCAGAATCCCTTTGTT TGGGATGTAAAGGCAATACAAGCTTTCAATGAATTTATAGATAATGCATGGCAAAAAAATCTAGAATTA AAATGTACAATATTTGCTCTGGCTTCAATTAATGAAGAACTGTTTAACATTGTGGATTTGCTAACCCCC TTTCAGAGTGCATGCCATTTCTTGGTAGAAAAGAGACTTGCAAGACCAGTAAAACTTCAGAAGCCTTTG GAGTCCTCTGTTCAGCTACATTCCTACTTCTATTCTACACATGATATGAAAATTGGAAGTGAAGAATTA GTTTATATAACGCATATTGATGACCCTTGGACATTTTATTGCCAGCTGGCAAGAAATGCAAATATTTTA GAACAGTTGTCATGTAGTATTACACAATTAAGTAAAGTTTTGCTGAATTTAAAAACATCTCCCTTGAAC CCTGGAACCTTGTGCCTTGCCAAGTATACTGATGGAAACTGGTATAGGGGCATAGTAATAGAGAAAGAG CCAAAGAAAGTCTTCTTTGTTGATTTTGGGAATATTTATGTAGTAACAAGTGATGATCTGCTTCCAATA CCTAGTGATGCATATGATGTCTTACTTTTGCCCATGCAAGCTGTCAGATGTTCATTATCTGATATTCCT GATCATATACCAGAAGAAGTGGTGGTGTGGTTTCAGGAGACTATTTTAGATAAGTCATTGAAGGCTTTA GTTGTAGCAAAAGATCCAGATGGAACACTGATTATAGAACTATATGGTGACAATATTCAAATTAGTGCT AGTATTAATAAGAAGTTGGGGCTACTTAGTTACAAAGATAGAATAAGAAAAAAAGAAAGTGAAGTCCTC TGTTCTACAACTGAAACTCTTGAAGAAAAAAATGAGAATATGAAGTTGCCATGTACAGAGTATTTAAGT AAATCAGTAGGGTACAAGTTACCTAATAAAGAAATTTTGGAAGAGTCATATAAACCTCAGATCAACTCA TCATACAAGGAACTCAAACTTTTACAAAGTTTAACAAAAACAAACTTAGTCACTCAATATCAAGACTCT GTGGGAAATAAAAATAGTCAAGTGTTTCCATTAACAACAGAAAAGAAAGAAGAAATTTCTGCTGAGACA CCCTTGAAAACAGCAAGAGTAGAAGCTACTCTTTCAGAGAGAAAAATAGGAGATTCATGTGACAAAGAT TTGCCTCTGAAATTTTGTGAGTTCCCACAGAAGACTATAATGCCTGGATTTAAAACAACTGTATATGTT TCTCATATAAATGACCTTTCAGACTTTTATGTTCAACTAATAGAAGATGAAGCTGAAATTAGTCATCTT TCAGAGAGATTAAACAGTGTTAAAACAAGGCCCGAATATTATGTAGGTCCACCTTTGCAAAGAGGAGAT ATGATATGTGCTGTTTTCCCAGAAGATAATTTATGGTATCGTGCTGTGATCAAGGAGCAACAACCCAAT GACCTTCTCTCTGTGCAGTTTATAGATTATGGCAATGTTTCTGTGGTTCATACTAACAAAATAGGTAGG CTTGACCTTGTTAATGCAATATTGCCGGGGTTGTGCATTCATTGCTCCTTGCAGGGATTTGAGGTTCCT GACAATAAAAATTCTAAGAAAATGATGCATTACTTTTCCCAACGGACCAGCGAGGCTGCAATAAGATGT GAATTTGTTAAATTTCAAGACAGATGGGAAGTTATTCTTGCTGATGAACATGGGATCATAGCAGATGAT ATGATTAGCAGGTATGCTCTCAGTGAAAAATCTCAAGTAGAACTTTCTACCCAAGTAATTAAAAGTGCC AGTTCAAAGTCTGTTAACAAATCAGACATTGACACTTCAGTATTTCTTAACTGGTATAATCCAGAAAAA AAAATGATAAGAGCTTATGCCACTGTGATAGATGGACCTGAGTACTTTTGGTGTCAGTTTGCTGATACG GAGAAACTTCAGTGTTTAGAAGTAGAAGTACAGACTGCTGGAGAACAGGTAGCAGACAGGAGAAATTGT ATCCCATGTCCTTATATTGGAGATCCTTGTATAGTAAGATACAGAGAAGATGGACATTATTATAGGGCA CTTATCACTAATATTTGTGAAGATTATCTTGTATCTGTCAGGCTTGTGGACTTTGGAAACATTGAAGAC TGTGTGGACCCAAAAGCACTCTGGGCCATTCCTTCTGAACTTCTGTCGGTTCCCATGCAAGCCTTTCCA TGTTGCCTCTCAGGGTTTAACATTTCAGAAGGATTATGTTCTCAAGAGGGAAATGACTATTTCTATGAA ATAATAACAGAAGATGTGTTGGAAATAACAATACTAGAAATCAGAAGGGATGTTTGTGATATCCCTTTA GCAATTGTTGACTTGAAAAGCAAAGGTAAAAGTATTAATGAGAAAATGGAGAAATATTCTAAGACTGGT ATTAAAAGTGCTCTTCCCTATGAAAATATTGACTCAGAGATAAAGCAGACTCTTGGGTCCTACAATCTT GATGTAGGACTTAAGAAATTAAGTAATAAAGCTGTACAAAATAAAATATATATGGAACAACAGACAGAT GAGCTTGCTGAAATAACTGAAAAAGATGTAAACATTATTGGAACCAAACCAAGTAACTTCCGTGACCCT AAAACTGATAACATTTGTGAAGGGTTTGAAAACCCCTGCAAAGATAAAATTGATACTGAGGAACTGGAA GGTGAATTAGAGTGCCATCTGGTTGACAAAGCAGAGTTTGATGATAAATACCTGATTACAGGATTTAAC ACATTACTACCACATGCTAATGAAACAAAGGAGATACTAGAACTGAATTCACTTGAGGTGCCGCTTTCT CCTGATGATGAATCAAAAGAATTCTTAGAACTGGAATCTATTGAGTTACAGAATTCTCTGGTGGTGGAT GAAGAAAAAGGGGAGCTAAGCCCGGTGCCACCGAATGTGCCACTCTCCCAAGAGTGTGTCACAAAAGGC GCCATGGAGCTATTTACACTGCAGCTTCCTCTCAGCTGTGAAGCTGAGAAACAGCCAGAACTAGAACTA CCTACAGCCCAGCTGCCTTTAGATGACAAGATGGATCCTTTGTCTTTAGGAGTTAGTCAGAAAGCACAG GAATCCATGTGTACTGAGGACATGAGAAAGTCAAGTTGTGTAGAATCTTTTGATGACCAGCGCAGGATG TCATTGCATCTACATGGAGCAGATTGTGATCCTAAAACACAGAATGAAATGAATATATGTGAAGAAGAA TTTGTAGAGTATAAAAACAGGGATGCCATTTCGGCATTGATGCCTTTGTTCTCTGAGGAAGAAAGCAGT GATGGAAGCAAGCACAATAATGGTTTACCAGATCATATCTCAGCTCAACTACAGAACACCTACACTCTG AAAGCCTTTACTGTTGGATCTAAATGTGTTGTGTGGTCAAGTCTAAGAAACACATGGTCTAAATGTGAG ATTTTAGAAACAGCTGAAGAAGGAACAAGGGTTTTGAACCTTTCAAATGGTATGGAGGAGATAGTGAAC CCTGAGAATGTCTGGAATGGCATACCCAAATTGGATAAGAGTCCACCTGAGAAAAGGGGTTTGGAGGTG ATGGAGATTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001010870
Insert Size	6291 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001010870.2
RefSeq Size	8977 bp
RefSeq ORF	6291 bp
Locus ID	221400
UniProt ID	O60522
MW	236.5 kDa
Gene Summary	This gene encodes a tudor domain-containing protein and component of the chromatoid body, a type of ribonucleoprotein granule present in male germ cells. Studies in rodents have demonstrated a role for the encoded protein in spermiogenesis and the nonsense mediated decay (NMD) pathway. This protein is a major autoantigen in human patients with autoimmune polyendocrine syndrome type 1 (APS1). [provided by RefSeq, Oct 2016] Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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