NWD1 (NM_001007525) Human Untagged Clone
CAT＃: SC301221
NWD1 (untagged)-Human NACHT and WD repeat domain containing 1 (NWD1)
CNY 27460.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC301221 representing NM_001007525.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCAGAGAGGGAAGCCCTGCAGAGCACTGCCTACCCTGAAGTGCCAGACCTTCTGCCAGAGGCACGGC TTGATGTTTGAGGTCGTTGATCTGAGGTGGGGTATTCGGAACATTGAAGCCACTGACCACTTGACCACA GAACTCTGCTTGGAGGAGGTTGACCGGTGTTGGAAAACATCCATAGGGCCAGCTTTTGTTGCCCTCATC GGTGATCAGTACGGCCCCTGTCTGATTCCCTCGCGGATCGATGAGAAGGAGTGGGAGGTATTGAGGGAC CATCTGACTGCCAGGCCAAGTGACCTGGAGCTGGTGGCACGATACTTCCAGAGGGACGAGAATGCGTTT CCTCCCACCTACGTCCTGCAGGCACCAGGTACTGGGGAGGCCTGTGAACCAGAGGAGGCCACCTTAACT TCTGTCCTACGCTCTGGAGCCCAGGAGGCCCGGAGGCTGGGGCTCATCACCCAGGAGCAGTGGCAGCAC TACCACCGGTCAGTCATTGAGTGGGAGATAGAGCGGAGCCTGCTGAGCTCAGAGGACCGGGAACAGGGA GCCACCGTCTTCCTTAGAGAGATCCAAGACCTCCACAAACACATCCTTGAAGACTGCGCCCTTAGGATG GTGGACCGGCTCGCGGATGGCTGCCTGGACGCTGATGCCCAGAACCTTCTCAGCAGCCTCAAAAGTCAC ATCACTGACATGCACCCAGGGGTCCTCAAGACCCACCGCCTGCCGTGGAGCCGCGACTTGGTGAACCCC AAGAACAAGACTCACGCCTGCTACCTGAAGGAGCTGGGTGAGCAGTTTGTGGTGAGGGCCAATCACCAG GTCCTCACACGCCTCCGTGAGCTGGATACGGCCGGACAGGAGTTGGCGTGGCTCTACCAAGAGATCCGC CACCACCTTTGGCAGAGCTCGGAGGTCATTCAGACCTTCTGCGGACGCCAGGAACTCCTGGCCCGGCTT GGGCAGCAGCTCAGGCACGATGACAGCAAGCAGCACACCCCCCTGGTACTCTTTGGGCCCCCAGGCATT GGAAAGACAGCCCTGATGTGCAAGCTGGCTGAGCAGATGCCAAGGCTGCTGGGGCACAAGACAGTGACC GTCCTGCGGCTGCTGGGGACGTCACAAATGAGCTCAGATGCCCGTGGCCTGCTGAAGAGCATCTGCTTC CAGGTGTGCCTGGCCTATGGGCTGCCCTTGCCCCCTGCCCAGGTTCTGGACGCCCACACCAGGGTGGTC CAGTTTTTCCATACCCTCCTCCACACTGTCTCTTGCAGAAACTTCGAGTCTCTCGTGCTCCTGCTGGAT GCTATGGATGACCTGGACTCTGTCCGCCATGCTCGGAGGGTTCCCTGGCTGCCTCTCAACTGCCCCCCG AGGGTGCACCTCATCCTCTCAGCTTGCTCGGGGGCACTGGGGGTTTTGGACACCTTGCAGCGGGTGCTC CTGGACCCGGAGGCCTACTGGGAGGTGAAGCCCCTTTCCGGAAACCAAGGCCAGCAGATGATCCAACTC CTGCTGGCAGCTGCAAGGAGGACGCTGAGCCCGGTGCACACAGATTTGCTCTGGGCCAGCCTCCCAGAG TGTGGGAACCCAGGGCGGCTGAGGCTGGCGTTTGAGGAAGCCCGGAAATGGGCCTCTTTCACCGTGCCT GTCCCGCTGGCCACCACCGCAGAGGAAGCCACGCACCAACTCTGCACCCGCCTGGAGCAGACACACGGG CAGCTCCTCGTGGCCCACGTGCTGGGCTACATTGTGTCTTCCCGACACGGTCTCTCGGAGGCGGAGCTG AAGGATGTTTTGTCCCTGGACGACGAGGTCCTGCAGGATGTGTACCGAGATTGGACCCCGCCCAGCAAG GAGCTGCTGCGCTTCCCGCCCCTGCTGTGGGTGCGGCTTCGTCGGGATCTGGGATACTACTTGGCCCGG CGGCCCGTGGATGGCTTCACCCTCCTGGCCATTGCCCACAGACAGCTGGTCGAGGTGGTCCGTGAGCGC TACCTGTCAGGATCCGAGAGAGCCAAGAGGCATGGCGTCCTGGCCGACTTCTTCTCAGGGACCTGGAGC CAGGGTACCAAGAAGCTCATCACTCTGCCACTTGTGGGGAAACCACTGAACTTGGACCGAAAGGTGGCC CCGCAGCCTCTGTGGTTCTCACATACGGTTGCAAACCTGCGGAAGCTGAAGGAGTTGCCCTATCACCTG CTTCACTCGGGCCGCCTGGAGGAGCTGAAACAGGAGGTTCTGGGCAGCATGAGCTGGATTTCCTGCCGG GGCATCTCTGGGGGCATTGAAGACCTGCTGGATGACTTTGACCTGTGTGCCCCTCACCTGGACTCCCCT GAGGTTGGCCTGGTCCGTGAAGCCCTCCAGCTCTGCCGCCCTGCTGTGGAGCTCCGAGGCATGGAGAGG AGCCTCCTGTACACAGAACTGCTGGCCAGACTCCATTTCTTCGCCACCTCACATCCAGCACTGGTGGGA CAGCTATGCCAACAGGCCCAGAGCTGGTTCCAGTTGTGCGCACACCCTGTGCTGGTGCCCCTCGGAGGA TTCCTCCAGCCCCCGGGAGGACCCCTCCGGGCAACTCTCAGCGGCTGTCACAAAGGCATCACCGCCATG GCATGGGGTGTGGAGGAGAAGCTGCTGGTGATTGGCACCCAGGATGGCATCATGGCTGTGTGGGACATG GAAGAGCAGCATGTGATCCACATGCTAACTGGACACACAGGAGAGGTGAGGTGTGTGAAAATATTTGCC AAAGGGACCCTCGCCAACTCTGCTTCAAAGGATTACACGCTGCACTTGTGGAACTTACTCTCTGGCCAG GAGAAATTTACCATTTGGGATGGAGGCTCAAAAAATCCCGCTGAACCTCAGATCTGGAACCTTCATGTG GATGAGGCACACAAAGTTGTGTATTCAGCATCTGGCTCAAAGATCAATGCTTGGAATCTGGAAACTGCA GAGCCGGTATTCCATATCCTGGGAGATGCCTCTGATCCTTGGATGTGCATGGCCGTGCTGGCCTCCCAG GCCACACTGCTGACAGTGTCCAGGGATGGTGTGGTCAGTCTGTGGAGCTCAGCTACGGGAAAACTTCAG GGGAAGCAACATATGTCCAGCATCAAAGAAGAAACACCTACCTGTGCCGTCTCAGTCCAGAAGCAAGGA AAGCTTGTTACCGGGTTTAGCAATGGCTCCATCTCTTTGGTTTCCTCCAAAGGGGACAGATTGCTGGAG AAGCTTCCAGATGCTGTGAGGTTCCTGGTGGTCTCTGAAGATGAGTCCCTCCTCGCCGCAGGCTTTGGA AGATCGGTGCGGATATTCTTGGCGGACTCGAGGGGCTTTCGCCGATTCATGGCCATGGATCTGGAACAT GAAGACATGGTGGAGACGGCTGTTTTTGGTACTGAGAACAACCTGATCATCACGGGGTCCCTTGATGCG CTCATTCAGGTGTGGAGTCTGTCAGAACAGGGGACCCTTCTGGACATCCTGGAAGGCGTCGGGGCCCCC GTGAGCCTGCTGGCCCGCGGCGGGGCTTTGGTGGCATCTGCTTCCCCACAGTCCTCATCTTTCAAGGTC TGGGATCTCAGCGATGCTCATAGGTCCCGGGTGCCTGCACCATTTCTGGACCGCACCGGCCTCACCGCA GTGTCCCACAATGGAAGCTACGTCTACTTCCCCAAAATTGGGGACAAAAACAAAGTCACTATTTGGGAC TTGGCAGAAGGCGAGGAACAAGATTCCCTGGACACCTCCAGTGAGATCAGGTGTCTGGAGGTTGCTGAG CAGCGCAAGCTCCTATTTACGGGCCTCGTGTCGGGGGTCGTCCTTGTGTTCCCCCTGAATTCCAGGCAG GACGTGATATGCATTCCCCCTCCCGAGGCCCGGAAAGCAATCAACTGCATGTCCCTGAGCAAGTGCGAG GACCGCCTGGCCATCGCCTATGACAACATCGTCCTGGTGCTGGACATCACCTCCGGGGACCCCTGCCCG GTCATCGATGGGCCAAGATACACCTTTTACACTCAGCTGCCCGAGACCCTCTCCAGCGTGGCCATTCTG ACGGACTACCGCGTGGTCTACAGCATGACCAATGGGGACCTCTTTCTTTACGAGTGTGCAACTTCCAAA GCGTTTCCCTTGGAGACCCACAGGAGCCGAGTTGCCTGTGTGGAGGTCAGCCACAAGGAGCAGCTGGTG GTCAGCGGGTCTGAGGATGCCCTGCTGTGTCTCTGGGACCTGCAGGCACGCAAGTGGAAATTCGAGATG AGCTACACGGCACCCTGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001007525 |
| Insert Size | 4299 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001007525.3 |
| RefSeq Size | 7645 bp |
| RefSeq ORF | 4299 bp |
| Locus ID | 284434 |
| UniProt ID | Q149M9 |
| MW | 159.9 kDa |
| Gene Summary | The protein encoded by this gene is thought to be a cytosolic protein and predicted to contain a NACHT domain and multiple WD40 repeats. Increased expression of this gene was observed in some prostate cancer cell lines. Knocking down expression of this gene results in decreased androgen receptor protein levels, indicating that this gene may be important in modulating androgen receptor activity. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2016] Transcript Variant: This variant (1) encodes the longest isoform (1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218768 | NWD1 (Myc-DDK-tagged)-Human NACHT and WD repeat domain containing 1 (NWD1) |
CNY 9800.00 |
|
| RC218768L3 | Lenti ORF clone of Human NACHT and WD repeat domain containing 1 (NWD1), Myc-DDK-tagged |
CNY 12730.00 |
|
| RC218768L4 | Lenti ORF clone of Human NACHT and WD repeat domain containing 1 (NWD1), mGFP tagged |
CNY 12730.00 |
|
| RG218768 | NWD1 (tGFP-tagged) - Human NACHT and WD repeat domain containing 1 (NWD1) |
CNY 11880.00 |
