VHLL (NM_001004319) Human Untagged Clone
CAT＃: SC300638
VHLL (untagged)-Human von Hippel-Lindau tumor suppressor-like (VHLL)
CNY 1200.00
CNY 3990.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | VHLP; VLP |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_001004319 edited
ATGCCCTGGAGAGCGGGGAACGGGGTGGGTTTAGAGGCCCAGGCGGGCACCCAGGAGGCA GGCCCAGAAGAGTACTGCCAGGAAGAGTTGGGCGCCGAGGAGGAGATGGCAGCCAGAGCA GCATGGCCTGTGCTGCGCTCTGTGAACTCACGCGAGCTCTCCCGGATCATCATCTGCAAT CACAGCCCACGAATCGTGCTGCCTGTGTGGCTCAACTACTATGGCAAGCTGCTGCCCTAC CTGACGCTGCTGCCCGGCAGGGACTTCCGCATCCACAACTTCCGAAGCCACCCTTGGCTC TTCAGAGATGCAAGGACACATGATAAGCTTCTGGTTAACCAAACTGAATTGTTTGTGCCA TCTTCCAATGTTAATGGACAGCCTGTTTTTGCCAACATCACACTGCAGTGTATACCCTGA >OriGene 5' read for NM_001004319 unedited
AAGTCAAAATTGTAAACGACTCACTATAGGCGGCCGCGNAATCATGCCCTGGAGAGCGGG GAACGGGGTGGGTTTAGAGGCCCAGGCGGGCACCCAGGAGGCAGGCCCAGAAGAGTACTG CCAGGAAGAGTTGGGCGCCGAGGAGGAGATGGCAGCCAGAGCAGCATGGCCTGTGCTGCG CTCTGTGAACTCACGCGAGCTCTCCCGGATCATCATCTGCAATCACAGCCCACGAATCGT GCTGCCTGTGTGGCTCAACTACTATGGCAAGCTGCTGCCCTACCTGACGCTGCTGCCCGG CAGGGACTTCCGCATCCACAACTTCCGAAGCCACCCTTGGCTCTTCAGAGATGCAAGGAC ACATGATAAGCTTCTGGTTAACCAAACTGAATTGTTTGTGCCATCTTCCAATGTTAATGG ACAGCCTGTTTTTGCCAACATCACACTGCAGTGTATACCCTGAGTCGACTCTAGATTGCG GCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCA GTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAA TTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTATGGGGTGGAGGGGG GTGGGGTAATGGAAGCAAGGGGCAAGTTTGGGAAGACAACCTGTAGGGCCCTGCGGGGGT CTATTTGGGAACCAAGGCTGGAGTGGCAGTGGGCACCAATCTTTGGGCTCACTGGCAATC TCCCGCCTCCCTGGGTTTCAAGCGATTTCTCCCTGGCCTAAGCCCTCCCGAGGTTGTTGG GGATTCCCAGGCATGCATGGACCAGGGCTCAGCTAAATTTTTGGTTTTTTTGGTAAAAGA CGGGGGTG |
| Restriction Sites | Please inquire |
| ACCN | NM_001004319 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001004319.1, NP_001004319.1 |
| RefSeq Size | 676 bp |
| RefSeq ORF | 420 bp |
| Locus ID | 391104 |
| UniProt ID | Q6RSH7 |
| Gene Summary | Von Hippel-Lindau (VHL) tumor suppressor protein is a component of an E3 ubiquitin ligase complex that selectively ubiquitinates the alpha subunit of the hypoxia-inducible factor (HIF) transcription factor for proteasome-mediated degradation. Inactivation of VHL causes VHL disease and sporadic kidney cancer. This gene encodes a VHL homolog that lacks one of two key domains necessary for VHL function. This gene may contribute to the regulation of oxygen homeostasis and neovascularization during placenta development. This gene is intronless, and can also be interpreted as a retrotransposed pseudogene of the VHL locus located on chromosome 3. However, the protein is represented in this RefSeq due to evidence in PMID:14757845 that strongly suggests it is translated. The same publication also indicates that this protein binds HIF alpha but fails to recruit the E3 ubiquitin ligase complex, and it therefore functions as a dominant-negative VHL protein and a protector of HIF alpha. [provided by RefSeq, Jan 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219675 | VHLL (Myc-DDK-tagged)-Human von Hippel-Lindau tumor suppressor-like (VHLL) |
CNY 1200.00 |
|
| RC219675L1 | Lenti ORF clone of Human von Hippel-Lindau tumor suppressor-like (VHLL), Myc-DDK-tagged |
CNY 3600.00 |
|
| RC219675L2 | Lenti ORF clone of Human von Hippel-Lindau tumor suppressor-like (VHLL), mGFP tagged |
CNY 5890.00 |
|
| RC219675L3 | Lenti ORF clone of Human von Hippel-Lindau tumor suppressor-like (VHLL), Myc-DDK-tagged |
CNY 5890.00 |
|
| RC219675L4 | Lenti ORF clone of Human von Hippel-Lindau tumor suppressor-like (VHLL), mGFP tagged |
CNY 5890.00 |
|
| RG219675 | VHLL (tGFP-tagged) - Human von Hippel-Lindau tumor suppressor-like (VHLL) |
CNY 2800.00 |
