SLC12A3 (NM_000339) Human Untagged Clone
CAT＃: SC300050
SLC12A3 (untagged)-Human solute carrier family 12 (sodium/chloride transporters), member 3 (SLC12A3), transcript variant 1
CNY 19950.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | NCC; NCCT; TSC |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC300050 representing NM_000339.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCAGAACTGCCCACAACAGAGACGCCTGGGGACGCCACTTTGTGCAGCGGGCGCTTCACCATCAGC ACACTGCTGAGCAGTGATGAGCCCTCTCCACCAGCTGCCTATGACAGCAGCCACCCCAGCCACCTGACC CACAGCAGCACCTTCTGCATGCGCACCTTTGGCTACAACACGATCGATGTGGTGCCCACATATGAGCAC TATGCCAACAGCACCCAGCCTGGTGAGCCCCGGAAGGTCCGGCCCACACTGGCTGACCTGCACTCCTTC CTCAAGCAGGAAGGCAGACACCTGCATGCCCTGGCCTTTGACAGCCGGCCCAGCCACGAGATGACTGAT GGGCTGGTGGAGGGCGAGGCAGGCACCAGCAGCGAGAAGAACCCCGAGGAGCCAGTGCGCTTCGGCTGG GTCAAGGGGGTGATGATTCGTTGCATGCTCAACATTTGGGGCGTGATCCTCTACCTGCGGCTGCCCTGG ATTACGGCCCAGGCAGGCATCGTCCTGACCTGGATCATCATCCTGCTGTCGGTCACGGTGACCTCCATC ACAGGCCTCTCCATCTCAGCCATCTCCACCAATGGCAAGGTCAAGTCAGGTGGCACCTACTTCCTCATC TCCCGGAGTCTGGGCCCAGAGCTTGGGGGCTCCATCGGCCTCATTTTCGCTTTCGCCAATGCCGTGGGT GTGGCCATGCACACGGTGGGCTTTGCAGAGACCGTGCGGGACCTGCTCCAGGAGTATGGGGCACCCATC GTGGACCCCATTAACGACATCCGCATCATTGGCGTGGTCTCGGTCACTGTGCTGCTGGCCATCTCCCTG GCTGGCATGGAGTGGGAGTCCAAGGCCCAGGTGCTGTTCTTCCTTGTCATCATGGTCTCCTTTGCCAAC TATTTAGTGGGGACGCTGATCCCCCCATCTGAGGACAAGGCCTCCAAAGGCTTCTTCAGCTACCGGGCG GACATTTTTGTCCAGAACTTGGTGCCTGACTGGCGGGGTCCAGATGGCACCTTCTTCGGAATGTTCTCC ATCTTCTTCCCCTCGGCCACAGGCATCCTGGCAGGGGCCAACATATCTGGTGACCTCAAGGACCCTGCT ATAGCCATCCCCAAGGGGACCCTCATGGCCATTTTCTGGACGACCATTTCCTACCTGGCCATCTCAGCC ACCATTGGCTCCTGCGTGGTGCGTGATGCCTCTGGGGTCCTGAATGACACAGTGACCCCTGGCTGGGGT GCCTGCGAGGGGCTGGCCTGCAGCTATGGCTGGAACTTCACCGAGTGCACCCAGCAGCACAGCTGCCAC TACGGCCTCATCAACTATTACCAGACCATGAGCATGGTGTCAGGCTTCGCGCCCCTGATCACGGCTGGC ATCTTCGGGGCCACCCTCTCCTCTGCCCTGGCCTGCCTTGTCTCTGCTGCCAAAGTCTTCCAGTGCCTT TGCGAGGACCAGCTGTACCCACTGATCGGCTTCTTCGGCAAAGGCTATGGCAAGAACAAGGAGCCCGTG CGTGGCTACCTGCTGGCCTACGCCATCGCTGTGGCCTTCATCATCATCGCTGAGCTCAACACCATAGCC CCCATCATTTCCAACTTCTTCCTCTGCTCCTATGCCCTCATCAACTTCAGCTGCTTCCACGCCTCCATC ACCAACTCGCCTGGGTGGAGACCTTCATTCCAATACTACAACAAGTGGGCGGCGCTGTTTGGGGCTATC ATCTCCGTGGTCATCATGTTCCTCCTCACCTGGTGGGCGGCCCTCATCGCCATTGGCGTGGTGCTCTTC CTCCTGCTCTATGTCATCTACAAGAAGCCAGAGGTAAATTGGGGCTCCTCGGTACAGGCTGGCTCCTAC AACCTGGCCCTCAGCTACTCGGTGGGCCTCAATGAGGTGGAAGACCACATCAAGAACTACCGCCCCCAG TGCCTGGTGCTCACGGGGCCCCCCAACTTCCGCCCGGCCCTGGTGGACTTTGTGGGCACCTTCACCCGG AACCTCAGCCTGATGATCTGTGGCCACGTGCTCATCGGACCCCACAAGCAGAGGATGCCTGAGCTCCAG CTCATCGCCAACGGGCACACCAAGTGGCTGAACAAGAGGAAGATCAAGGCCTTCTACTCGGATGTCATT GCCGAGGACCTCCGCAGAGGCGTCCAGATCCTCATGCAGGCCGCAGGTCTCGGGAGAATGAAGCCCAAC ATTCTGGTGGTTGGGTTCAAGAAGAACTGGCAGTCGGCTCACCCGGCCACAGTGGAAGACTACATTGGC ATCCTCCATGATGCCTTTGATTTCAACTATGGCGTGTGTGTCATGAGGATGCGGGAGGGACTCAACGTG TCCAAGATGATGCAGGCGCACATTAACCCCGTGTTTGACCCAGCGGAGGACGGGAAGGAAGCCAGCGCC AGAGGTGCCAGGCCATCAGTCTCTGGCGCTTTGGACCCCAAGGCCCTGGTGAAGGAGGAGCAGGCCACC ACCATCTTCCAGTCGGAGCAGGGCAAGAAGACCATAGACATCTACTGGCTCTTTGACGATGGAGGCCTC ACCCTCCTCATTCCCTATCTCCTTGGCCGCAAGAGGAGGTGGAGCAAATGCAAGATCCGTGTGTTCGTA GGCGGCCAGATTAACAGGATGGACCAGGAGAGAAAGGCGATCATTTCTCTGCTGAGCAAGTTCCGACTG GGATTCCATGAAGTCCACATCCTCCCTGACATCAACCAGAACCCTCGGGCTGAGCACACCAAGAGGTTT GAGGACATGATTGCACCCTTCCGTCTGAATGATGGCTTCAAGGATGAGGCCACTGTCAACGAGATGCGG CGGGACTGCCCCTGGAAGATCTCAGATGAGGAGATTACGAAGAACAGAGTCAAGTCCCTTCGGCAGGTG AGGCTGAATGAGATTGTGCTGGATTACTCCCGAGACGCTGCTCTCATCGTCATCACTTTGCCCATAGGG AGGAAGGGGAAGTGCCCCAGCTCGCTGTACATGGCCTGGCTGGAGACCCTGTCCCAGGACCTCAGACCT CCAGTCATCCTGATCCGAGGAAACCAGGAAAACGTGCTCACCTTTTACTGCCAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_000339 |
| Insert Size | 3093 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000339.2 |
| RefSeq Size | 5582 bp |
| RefSeq ORF | 3093 bp |
| Locus ID | 6559 |
| UniProt ID | P55017 |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 113.9 kDa |
| Gene Summary | This gene encodes a renal thiazide-sensitive sodium-chloride cotransporter that is important for electrolyte homeostasis. This cotransporter mediates sodium and chloride reabsorption in the distal convoluted tubule. Mutations in this gene cause Gitelman syndrome, a disease similar to Bartter's syndrome, that is characterized by hypokalemic alkalosis combined with hypomagnesemia, low urinary calcium, and increased renin activity associated with normal blood pressure. This cotransporter is the target for thiazide diuretics that are used for treating high blood pressure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221919 | SLC12A3 (Myc-DDK-tagged)-Human solute carrier family 12 (sodium/chloride transporters), member 3 (SLC12A3), transcript variant 1 |
CNY 7176.00 |
|
| RC221919L1 | Lenti ORF clone of Human solute carrier family 12 (sodium/chloride transporters), member 3 (SLC12A3), transcript variant 1, Myc-DDK-tagged |
CNY 9576.00 |
|
| RC221919L2 | Lenti ORF clone of Human solute carrier family 12 (sodium/chloride transporters), member 3 (SLC12A3), transcript variant 1, mGFP tagged |
CNY 12160.00 |
|
| RC221919L3 | Lenti ORF clone of Human solute carrier family 12 (sodium/chloride transporters), member 3 (SLC12A3), transcript variant 1, Myc-DDK-tagged |
CNY 12160.00 |
|
| RC221919L4 | Lenti ORF clone of Human solute carrier family 12 (sodium/chloride transporters), member 3 (SLC12A3), transcript variant 1, mGFP tagged |
CNY 9576.00 |
|
| RG221919 | SLC12A3 (tGFP-tagged) - Human solute carrier family 12 (sodium/chloride transporters), member 3 (SLC12A3), transcript variant 1 |
CNY 11310.00 |
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