DEP1 (PTPRJ) (NM_002843) Human Untagged Clone

CAT#: SC128010

PTPRJ (untagged)-Human protein tyrosine phosphatase, receptor type, J (PTPRJ), transcript variant 1



  "NM_002843" in other vectors (6)

CNY 13,728.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
Rabbit polyclonal His6-DEP-1 Antibody
    • 400 ul

CNY 6,160.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms CD148; DEP1; HPTPeta; R-PTP-ETA; SCC1
Vector pCMV6-XL6
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_002843 edited
GGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCATTTAGGTGACACTAT
AGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGCGGAGGAGG
AGGCGAAGGAGACGGCAGGAGGCGGCGACGACGGTGCCCGGGCTCGGGCGCACGGCGGGG
CCCGATTCGCGCGTCCGGGGCACGTTCCAGGGCGCGCGGGGCATGAAGCCGGCGGCGCGG
GAGGCGCGGCTGCCTCCGCGCTCGCCCGGGCTGCGCTGGGCGCTGCCGCTGCTGCTGCTG
CTGCTGCGCCTGGGCCAGATCCTGTGCGCAGGTGGCACCCCTAGTCCAATTCCTGACCCT
TCAGTAGCAACTGTTGCCACAGGGGAAAATGGCATAACGCAGATCAGCAGTACAGCAGAA
TCCTTTCATAAACAGAATGGAACTGGAACACCTCAGGTGGAAACAAACACCAGTGAGGAT
GGTGAAAGCTCTGGAGCCAACGATAGTTTAAGAACACCTGAACAAGGATCTAATGGGACT
GATGGGGCATCTCAAAAAACTCCCAGTAGCACTGGGCCCAGTCCTGTGTTTGACATTAAA
GCTGTTTCCATCAGTCCAACCAATGTGATCTTAACTTGGAAAAGTAATGACACAGCTGCT
TCTGAGTACAAGTATGTAGTAAAGCATAAGATGGAAAATGAGAAGACAATTACTGTTGTG
CATCAACCATGGTGTAACATCACAGGCTTACGTCCAGCGACTTCATATGTATTCTCCATC
ACTCCAGGAATAGGCAATGAGACTTGGGGAGATCCCAGAGTCATAAAAGTCATCACAGAG
CCGATCCCAGTTTCTGATCTCCGTGTTGCCCTCACGGGTGTGAGGAAGGCTGCTCTCTCC
TGGAGCAATGGCAATGGCACCGCCTCCTGCCGGGTTCTTCTTGAAAGCATTGGAAGCCAT
GAGGAGTTGACTCAAGACTCAAGACTTCAGGTCAATATCTCGGGCCTGAAGCCAGGGGTT
CAATACAACATCAACCCGTATCTTCTACAATCAAATAAGACAAAGGGAGACCCCTTGGGC
ACAGAAGGTGGCTTGGATGCCAGCAATACAGAGAGAAGCCGGGCAGGGAGCCCCACCGCC
CCTGTGCATGATGAGTCCCTCGTGGGACCTGTGGACCCATCCTCCGGCCAGCAGTCCCGA
GACACGGAAGTCCTGCTTGTCGGGTTAGAGCCTGGCACCCGATACAATGCCACCGTTTAT
TCCCAAGCAGCGAATGGCACAGAAGGACAGCCCCAGGCCATAGAGTTCAGGACAAATGCT
ATTCAGGTTTTTGACGTCACCGCTGTGAACATCAGTGCCACAAGCCTGACCCTGATCTGG
AAAGTCAGCGATAACGAGTCGTCATCTAACTATACCTACAAGATACATGTGGCGGGGGAG
ACAGATTCTTCCAATCTCAACGTCAGTGAGCCTCGCGCTGTCATCCCCGGACTCCGCTCC
AGCACCTTCTACAACATCACAGTGTGTCCTGTCCTAGGTGACATCGAGGGCACGCCGGGC
TTCCTCCAAGTGCACACCCCCCCTGTTCCAGTTTCTGACTTCCGAGTGACAGTGGTCAGC
ACGACGGAGATCGGCTTAGCATGGAGCAGCCATGATGCAGAATCATTTCAGATGCATATC
ACACAGGAGGGAGCTGGCAATTCTCGGGTAGAAATAACCACCAACCAAAGTATTATCATT
GGTGGCTTGTTCCCTGGAACCAAGTATTGCTTTGAAATAGTTCCAAAAGGACCAAATGGG
ACTGAAGGGGCATCTCGGACAGTTTGCAATAGAACTGGTAAGCAAATAGGCTTTTCTGTT
AAACCATCATGTTTCTTAAAGGAAATCAGTATAATTAATAAACATAATGTTTCCAAAAAA
AAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAAC
AGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGC
CACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTA
GGTGTCCTTCTATAATATAT
>OriGene 5' read for NM_002843 unedited
ATTTCCCCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAG
CAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGC
GAATTCGGCACGAGGCGGAGGAGGAGGCGAAGGAGACGGCAGGAGGCGGCGACGACGGTG
CCCGGGCTCGGGCGCACGGCGGGGCCCGATTCGCGCGTCCGGGGCACGTTCCAGGGCGCG
CGGGGCATGAAGCCGGCGGCGCGGGAGGCGCGGCTGCCTCCGCGCTCGCCCGGGCTGCGC
TGGGCGCTGCCGCTGCTGCTGCTGCTGCTGCGCCTGGGCCAGATCCTGTGCGCAGGTGGC
ACCCCTAGTCCAATTCCTGACCCTTCAGTAGCAACTGTTGCCACAGGGGAAAATGGCATA
ACGCAGATCAGCAGTACAGCAGAATCCTTTCATAAACAGAATGGAACTGGAACACCTCAG
GTGGAAACAAACACCAGTGAGGATGGTGAAAGCTCTGGAGCCAACGATAGTTTAAGAACA
CCTGAACAAGGATCTAATGGGACTGATGGGGCATCTCAAAAACTCCCAGTAGCACTGGGC
CCAGTCCTGTGTTTGACATTAAAGCTGTTTCCATCAGTCCAACCAATGTGATCTTAACTT
GGAAAAGCAATGACACAGCTGCTTTCTGAGTACAAGTATGTTAGTAAAGCACTAAGATGG
GAAATGGAGAAGACAATTACTTGTGTGGATCAACCATGGTGGAACATTACAGGCTTACCG
TCAGCGACCTTATATGGATTCCCTCTTCCTTCAGGAAATAGCAATGACACCTCGGCNAGA
ACCCCACAGTCATTAAAAGTCATTACCGGAGCCGATCCCAGCTTCCTGATTCTCCGGTGT
GCCCTCCCCGGGTGGGAGGGAAGGTTGGCTCCCTCCTGAAGCCATGCCAAAGGGCACCCG
CCTCCTGCCGGTTNTTT
>OriGene 3' genomic read for NM_002843 unedited
NAAAATTTACTGTGNACCGCGGCCCGCTTTCGANGATCGAGTTTTTTTTTTTTTTTTTTA
ATGCATTGACATTTTTTTATATTTGGTATTTGTAAGTCTTTAAAAAAAGTTTTCAGGCCA
TTTTTTCCTTAAAAAAAAAAAAAAGCAACCAACAGCAATACTCTGTACAAGTATAACAAA
CATTAGAAATATGCATCATTCCAAAATAGTTACAGGGAAAATTACAGTTTAGAGTCCACA
TCAACACATCCTATTTGTATGTGCCCCCAAGGGAGAAAAAGCTACAGTATGTTAAACACA
CAGCTGCTACACAGTAGTCTGAAAACCCAGGACTTAAAACTTTTGAGGCAAATCAACAAC
AGTCACCAAGACTTGTTTAGCTCAATAAGTACAATCAAGCATTTCAAAAGAGAACCAGGC
TTTTTCATCCCAGATGAAAAACACACGTGATGGGCTGCATAGCTGACCCCCGCCCCTCGA
TCCCCAACCCCCGGGAGCCTCTGACTCAACAGAGCACAAATCCAGTGGAGTCAATTAGGC
GGAAGTCTGGCTGGAGGTCACAGTATGGCTGCAGTGGGCAGACACCAAATGTCATTTCCT
GCTGGTGCTGATCATCACTAGATCCAACTTTGGAGGCAAAAAGTCGTTGTTCCTGGGAGA
TGCTATCAATGGCCCAAATCCCCCAAACCAGCTGGCCACCAGTCTTTGGCCCTTTCATTG
ACACCCAAACCCATACCAGCTGCTTTTCTGTATGCAATTGTCAAAGACTGAACAGGGGCG
CTCACACCATCCCCCCGATTCCACTGGACTCTTCCATTNNGAGCTCATGNTCCTGGGAGG
GGGTGAAGCTCANAGCACCCTNCTCTTGGCTCTGACCAACCCCAGCCTGTGTGTCCTTTT
ATATTAACATT
Restriction Sites NotI-NotI     
ACCN NM_002843
Insert Size 9000 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002843.2, NP_002834.2
RefSeq Size 5119 bp
RefSeq ORF 4014 bp
Locus ID 5795
UniProt ID Q12913
Domains Y_phosphatase, PTPc_motif, FN3
Protein Families Druggable Genome, Phosphatase, Transmembrane
Protein Pathways Adherens junction
Gene Summary The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes, including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region containing five fibronectin type III repeats, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. This protein is present in all hematopoietic lineages, and was shown to negatively regulate T cell receptor signaling possibly through interfering with the phosphorylation of Phospholipase C Gamma 1 and Linker for Activation of T Cells. This protein can also dephosphorylate the PDGF beta receptor, and may be involved in UV-induced signal transduction. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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