Kaiso (ZBTB33) (AK092998) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ZNF-kaiso; ZNF348
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK092998, the custom clone sequence may differ by one or more nucleotides TTTATGTCTTCACATATAAAGTCAGTTCATAGTCAAGATCCTTCTGGGGACTCAAAGCTTTATCGTTTAC ATCCATGCAGGTCTTTACAAATCAGACAATATGCATATCTTTCCGATAGATCAAGCACTATTCCTGCAAT GAAGGATGATGGTATTGGGTATAAGGTTGACACTGGAAAAGAACCTCCAGTAGGGACCACTACATCTACT CAGAACAAGCCAATGACCTGGGAAGATATTTTTATTCAGCAGGAAAATGATTCAATTTTTAAACAAAATG TAACAGATGGCAGTACTGAGTTTGAATTTATAATACCAGAGTCTTACTAAACTCCTTTGAAATACTAGAA AGTTTTGTTTTGGATGATGGGGCAGGGGTTTCAGAAGATCTGTAAAACAAATTAAGGTGCGAACAAGTTA ATTTGATCTGCCACATTATCTGAAGGAAGTGTAGTGGGATTTTTGTTGATAATTTTTAGAAGCAAATTTT CCTGAAAGTTTTGAGTAGAGGTGAGACCCCCTCCCCAAGTATCTGTTTATATAGTTAGTTTTCAGCTCAT TTAAAGGAGGCAAAAATTAAAAGCTTGGAGAGATAGTTTCCTGAATAGAATTTGAAGCAGTCTGAATGTT CTTTGAAAATAACTGGAGTTATTAGCATACCCTAGTACATCTTACAGCTTTCCCCTTCCATGTTAGCACT TTACTGCTGAATTCTCAATTTTCTTAACATTGAGACAATAAATGTGTGTTTTGTCTTGTATATGGCATAA AGAGTAAATAAGTTTTAGAGTTGTTCTGGAAAATGTCAGAATAAGTCAGTACTTGGGTTGTGTAATCTGC TAGTCCAAGCGAACAGCAACCTCCTGCTACCCTCCCTCTATGAAAATAGCCATGCAGACAAGTCTCTCAT CTGAAGAACAAATTAGATTTAGCTAATTAGAATTAATCCTGGCTTTCATTGCCATAGTCTGTAAAAGACT TTGGTGGCTAGACCACTTTATACCTTTGCAGTGTGGTCTCTGGGGGCAAAAAACTAATGAAAACAATCTC TGTAATGGCAGATAGGAGGAGATGAAAAGTTCTGTTGCATGGATTTTTAATTCTCTGGCTACCACATAGT AGAGAATGGAATGAAGATTTCCTTTTGGCTTCTTAAGGTTAAAAATATTCCCATGAACATGAAAATTTTC AAATTTTGAATCTGAAAGCCACCAAATGTATCTTTATGTATAAATCCTTGTAAATGATAGATTCCATGGG TGAGACTTTACATATTTTGGGTGGGAGGCTACTGGCATATATTTTTAAATGTTCATATTGCGTAGAATCT CCACTAGGAAGTCTTTATTTGAAATAGTTGAATCAGTGATCTAGTATTTTCCTTTCGGCAAGATTTGTTA GGTTTTTACCCCTTCTAAAATAAGTTTTATTCCATCTGCAAATTGCTGCAATATTATAGTAATCAGAAAC TACATAAGGAATGTTATATAGGCTTGTCAGTTCCCGTTTTTCTTGACAACAATAAATACCACTTTTAAAA ATGACACATATTTAAACACTTAGAAAATAAAGTTAACACTTACTGAAGTGCTAGTACTAAACTGTGCTAG TACTAAAAGAAAACAGGTTGGAACATACATATAGCCTAGCATTTATAACAGAATTGTTGAACGTCTGTAA ATGATTTCTTTTTTTTTTGCAAAGGAAAAAATTGATACTGGAAAAGATTGTTGTGCATAGTTATTAGTCA TTTGTAACCTTGCTTAAGTATTTCTTAGTCCAACATAGATATTTTCTTTCTCCTGACCATGTATTTTAAA ATATAGTCTATTTCTTGACTTTGAACTTAAAGCTTTAATCATAATTTCTCATGTATACATCGTTCTTCTG ATGGTAAGCTGGATTTGAAGGTAGTGGTTTCAGTGTTTCTTAAGTTGGTAGCTGAGGGTATCAGGCATCA GTTCATGCAATAATACAAGAAAAAAAATCCTTTGCTTGCCAAGAGGTAGAGTGATGTGCATTTATCTGTT TTCTGTTCTGTAAGTCTAGACCTTCAAACCATTTGTAAACTAACCCCTGGGAAATTTGAAATTACCTGAT AACTTAAGACTCTGTGATCTCTGGAATCACCATATGTTTCTTTTTTGTGTAGATATTAATAACATTACTC TTTGACTATAGTGTGCACTCTGAAATGTACTCAGTGAAAATTTGTTTTGAGTTTCATTAATGCTATTTCA CCAGTTAGACATAATTACTTCTACCGATGTGAATGATACGGATGCCGGCAGAGCTTCCAGATCTTTCAGA CTCAACTGCTAGGTCAATTAGTTTGTCATAATAAAACTTGGCAGATTCTACAAGTCTATTATGACAAACC AGGAACTAATTCTATAATGGAAAACTATCCATTCTGAATAATAGGTATGTAATTATTTGCTGCTGCTGCT GTGCTCTGTAAATTCTGAATATGACATTTAAACTCTGTGCCTACTAAAGGTATCTTCTGGAGTTTTTGGG AGGAGAGAAACTGGAAAATTAAATTGTATTTTTGCCAGAAGACTCTTACTTGCATGTGTCTCAGGGTCTT CAGTTTTTCTATAAGTTTCCATATCCAAAGTTCAGAATTCATGTGAAATACTTCTTTGGGGCAAAAGTCC TTCATTCCTGGTATTTATTGGATTGGAAATCTGTAGCAAGATGCTGTTTAAAATTACCATATTGTTTTTT TATCTTATACTTAGCTCTCTGGCTATTGAACTTCCTTTTCTTGTTTGAAGTTAGCTTCAAATTTGCTCCT ATGCTAAATTACCTGTAAATATTCTGGATAGGAACTACTTGAAATAGTAATTTGTTAAAAGATATGACAA AATGAAAATGCTTAAACTACAGAAATTTAAAAATGCCATAACAATCTTGCAAGACTAACTTTAAAATATA CTTTAAATGATTATTATGATTTTGGTGGTAACGATCCCCCACACACAACCACTATGAAGAAATAATGCCG CATTTTTCCCCCATTGTACC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK092998 unedited CGCGAATTCGGCACCAGCCCTGATGAAGGGGAGGCCATACTTGAGAATGAAATACCAAAA ACGTCTGGCAGCGAGATGGCAAACAAACGTATGAAAGTAAAACATGATGATCACTATGAG TTAATAGTAGATGGAAGGGTCTATTATATCTGTATTGTATGCAAAAGGTCATATGTCTGT CTGACAAGCTTGCGGAGACATTTTAACATTCATTCTTGGGAGAAGAAGTATCCGTGCCGT TACTGTGAGAAGGTATTTCCTCTTGCAGAATATCGCACAAAGCATGAAATTCATCACACA GGGGAGCGAAGGTATCAGTGTTTGGCCTGTGGCAAATCTTTCATCAACTATCAGTTTATG TCTTCACATATAAAGTCAGTTCATAGTCAAGATCCTTCTGGGGACTCAAAGCTTTATCGT TTACATCCATGCAGGTCTTTACAAATCAGACAATATGCATATCTTTCCGATAGATCAAGC ACTATTCCTGCAATGAAGGATGATGGTATTGAGTATAAGGTTGACACTGGAAAAGAACCT CCAGTAGGGACCACTACATCTACTCAGAACAAGCCAATGACCTGNGAAGATATTTTTATT CAGCAGGAAAATGATTCAATTTTTAAACAAAATGTAACAGATGGCAGTACTGAGTTTGAA TTTATAATACCAGAGTCTTACTAAACTCCTTTGAAATACTAGAAAGTTNNTGTTTGGATG ATGG
Restriction Sites	NotI-NotI
ACCN	AK092998
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK092998.1
RefSeq Size	3030 bp
RefSeq ORF	3030 bp
Locus ID	10009
Gene Summary	This gene encodes a transcriptional regulator with bimodal DNA-binding specificity, which binds to methylated CGCG and also to the non-methylated consensus KAISO-binding site TCCTGCNA. The protein contains an N-terminal POZ/BTB domain and 3 C-terminal zinc finger motifs. It recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. It may contribute to the repression of target genes of the Wnt signaling pathway, and may also activate transcription of a subset of target genes by the recruitment of catenin delta-2 (CTNND2). Its interaction with catenin delta-1 (CTNND1) inhibits binding to both methylated and non-methylated DNA. It also interacts directly with the nuclear import receptor Importin-α2 (also known as karyopherin alpha2 or RAG cohort 1), which may mediate nuclear import of this protein. Alternatively spliced transcript variants encoding the same protein have been identified.[provided by RefSeq, May 2010]

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