IBTK (NM_015525) Human Untagged Clone
CAT＃: SC127308
IBTK (untagged)-Human inhibitor of Bruton agammaglobulinemia tyrosine kinase (IBTK)
CNY 13896.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BTBD26; BTKI |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_015525, the custom clone sequence may differ by one or more nucleotides
ATGAGTTCACCCATGCCTGACTGCACATCAAAGTGTCGATCCCTGAAGCATGCTTTGGATGTCCTTTCTG TGGTAACAAAGGGGAGCGAAAACCAGATTAAGGCCTTTCTCTCCAGTCATTGTTACAATGCTGCAACTAT CAAGGATGTTTTTGGCAGGAATGCCCTCCACCTTGTTTCCTCCTGTGGAAAGAAAGGAGTGTTAGATTGG CTTATTCAGAAAGGAGTGGATCTGTTGGTGAAAGACAAAGAGTCTGGATGGACAGCATTGCACAGAAGCA TTTTTTATGGACATATTGATTGTGTTTGGTCTCTATTGAAGCATGGTGTTAGTCTGTATATTCAAGATAA AGAAGGCTTGTCAGCTTTGGATCTTGTAATGAAGGATAGACCAACTCATGTAGTATTCAAGAATACTGAT CCTACAGATGTTTATACTTGGGGCGATAATACAAATTTTACCCTGGGTCATGGAAGCCAGAATAGCAAAC ATCATCCAGAGTTGGTGGATCTGTTCTCCAGGAGTGGGATTTATATCAAGCAGGTGGTGCTTTGTAAATT TCACTCCGTGTTTCTGTCTCAGAAAGGGCAGGTTTATACCTGTGGTCATGGTCCTGGAGGGCGATTAGGA CATGGAGATGAACAGACATGCTTGGTCCCTCGGCTTGTGGAAGGACTGAATGGTCATAATTGTTCCCAAG TGGCAGCTGCTAAGGATCATACTGTTGTATTAACTGAAGATGGATGTGTTTATACATTTGGTCTAAACAT TTTTCATCAATTAGGAATTATTCCACCGCCTTCCAGTTGTAATGTACCCAGACAGATACAGGCAAAATAT CTGAAAGGAAGGACAATCATTGGCGTTGCAGCAGGCAGGTTTCATACAGTCCTATGGACTAGAGAAGCTG TTTACACTATGGGACTAAATGGTGGACAACTGGGTTGTTTGCTAGATCCCAATGGAGAAAAGTGTGTAAC TGCTCCTCGTCAGGTCTCTGCCCTTCACCATAAAGACATTGCTCTGTCTTTGGTTGCTGCAAGTGATGGA GCTACAGTCTGTGTTACCACAAGGGGAGATATTTACTTACTTGCAGACTATCAGTGCAAGAAGATGGCTT CTAAACAGTTGAACTTGAAAAAAGTTCTTGTGTCTGGGGGTCATATGGAATACAAGGTTGATCCTGAACA TTTGAAAGAAAATGGGGGTCAAAAAATTTGCATTCTTGCAATGGATGGAGCTGGAAGGGTGTTTTGCTGG AGATCAGTCAACAGTTCTCTGAAGCAGTGTCGATGGGCCTATCCACGTCAGGTCTTCATTTCTGATATTG CTTTAAATAGAAATGAAATTCTATTTGTTACGCAAGATGGAGAAGGATTTAGAGGGAGATGGTTTGAAGA GAAAAGAAAGAGTTCTGAAAAGAAAGAGATTTTATCAAACCTTCACAATTCCTCATCAGATGTGTCTTAT GTCTCTGATATAAATAGTGTGTATGAAAGAATTCGACTTGAGAAACTTACCTTTGCACATAGAGCTGTTA GTGTCAGCACAGATCCAAGTGGATGCAACTTTGCAATCCTGCAGTCAGATCCTAAAACAAGCCTTTATGA AATTCCAGCTGTGTCCTCATCATCCTTTTTTGAAGAGTTTGGCAAACTGTTGAGGGAAGCAGATGAAATG GACAGCATTCATGATGTGACATTTCAAGTTGGCAATAGACTCTTCCCTGCACATAAATATATTTTGGCAG TGCATTCTGATTTTTTTCAGAAATTGTTTCTTTCAGATGGTAATACTTCAGAATTTACAGATATTTACCA GAAAGATGAAGATTCTGCAGGGTGCCATCTCTTTGTGGTAGAGAAGGTTCATCCTGACATGTTTGAATAC CTTTTACAATTTATATACACAGATACTTGTGACTTTTTAACTCATGGCTTCAAACCAAGAATACACTTAA ACAAAAACCCAGAAGAATATCAGGGAACTCTGAATTCTCATTTGAATAAAGTGAATTTCCATGAAGATGA TAACCAGAAGTCTGCATTTGAAGTTTACAAAAGTAATCAAGCTCAAACAGTTAGTGAGAGGCAGAAGAGC AAACCTAAATCTTGTAAAAAAGGAAAAAATATTAGGGAAGATGATCCTGTAAGAATGTTGCAAACTGTTG CAAAGAAATTCGACTTCAGTAATTTGAGTAGTAGGTTAGATGGAGTCAGATTTGAAAATGAAAAAATTAA TGTTATTGCCAAGAACACTGGTAATAAACTGAAGCTAAGTCAGAAAAAATGTTCATTTCTGTGTGACGTG ACCATGAAATCAGTGGATGGAAAGGAATTTCCTTGTCATAAATGTGTTCTTTGTGCTAGACTTGAATATT TTCATAGTATGCTGAGTAGCTCATGGATTGAGGCTTCCAGTTGTGCAGCTCTGGAAATGCCAATACATTC TGATATATTAAAAGTTATTTTGGACTACCTCTATACTGATGAAGCTGTGGTGATAAAAGAATCTCAAAAT GTAGATTTTATTTGTAGTGTTCTTGTGGTGGCTGATCAACTTCTCATAACCCGGTTGAAAGAGATTTGTG AAGTAGCATTAACTGAAAAACTTACCCTGAAGAATGCTGCTATGCTACTGGAATTTGCAGCAATGTATAG TGCAAAACAGTTGAAACTGTCTTGTTTACAGTTTATAGGATTGAATATGGCAGCTTTACTTGAAGCAAGG TCTCTTGATGTTTTAAGCGATGGTGTTTTGAAGGATCTTTCTGAGTTTTACCGGAAAATGATTCCAGCAA TGGATAGAAGAGTCATTACACCATATCAAGATGGACCAGATATTAGCTATTTGGAAGTAGAAGATGGAGA TATCTTCTTGAAAGAAGAAATAAATATGGAACAAAATCATTCGGAAACTATGTTCAAGAAAGCAAAAACA AAAGCTAAAAAGAAGCCACGTAAACGTTCAGATAGTTCTGGAGGTTATAACCTTTCAGATATTATTCAGA GTCCATCATCTACAGGATTATTAAAGTCTGGTAAGACCAATTCTGTGGAATCTCTTCCAGAACTGTTGAC ATCAGACTCTGAAGGAAGCTATGCAGGAGTGGGTAGTCCTAGAGATTTACAGTCCCCTGATTTCACAACA GGATTTCATTCAGATAAGATTGAGGCGAAAGTCAAACCGTATGTTAATGGTACATCTCCTGTGTATTCTA GGGAAGATTTAAAACCATGGGAAAAGTCACCAATACTTAAAATATCTGCTCCACAGCCTATTCCCAGTAA CAGAATTGATACTACCAGCTCTGCCAGTTGGGTTGCTGGTTCTTTCAGTCCTGTCAGCCCTCCTGTTGTG GATCTCAGAACTATCATGGAAATAGAAGAAAGTAGACAAAAATGTGGAGCTACACCAAAGTCACATTTAG GCAAAACAGTTTCTCATGGAGTTAAACTTTCTCAGAAGCAACGAAAAATGATTGCATTGACTACCAAGGA AAACAATTCAGGAATGAATAGCATGGAAACAGTTTTATTCACTCCTTCAAAAGCCCCCAAACCAGTGAAT GCATGGGCATCTTCTCTGCATTCAGTTTCATCCAAGTCATTCCGGGATTTCTTACTAGAAGAAAAAAAGT CTGTTACTAGCCATAGTTCAGGCGATCATGTCAAAAAAGTTTCTTTTAAAGGAATTGAAAATTCTCAGGC ACCAAAAATTGTCAGATGCTCTACCCATGGTACCCCAGGACCAGAAGGCAACCATATTTCAGATTTACCA CTTCTAGACAGTCCCAATCCCTGGCTATCTTCTTCAGTGACTGCTCCATCCATGGTAGCCCCAGTCACTT TTGCATCTATTGTAGAAGAAGAACTACAACAAGAAGCAGCTCTTATTAGAAGTCGAGAAAAACCGTTGGC TCTGATTCAGATTGAGGAGCATGCCATACAAGATTTATTGGTTTTCTATGAGGCATTTGGCAACCCTGAA GAGTTTGTCATTGTTGAAAGGACACCGCAGGGACCACTGGCAGTACCTATGTGGAATAAGCATGGATGCT AG >OriGene 5' read for NM_015525 unedited
GTATACGACTCCTATAGGCGGCCGCGAATTCGGCACGAGGCTGTATTGCAGCCGCCTGCC GAACTGACCCGGGTCTGGGGACTGGCCCCTCTGGCGCCGTTCGGTTTCTCTTATTGCCTT CACTGAGGATGAGTCCCTTTGTGGCTCTATGTGGACCCTGCGGAATCCACCGGCGCAGTT TCATCTAGCGACTGGTCACCCTTGCAATTATGGATATTTAAAAGGGTCAGACATGTGGAT GAGGGGGAGTTCCCCTTCTCACTCCCCCTTGGTGCTTGACTCCAGGAATAATATATAAAC TGTGGAATTTTTTTAAATGAAGAACTTGTATTTGATATGAACTGTATAGAGATATTTATA ATTTTTTTGATTTAAGTGCCAAAAAATTGTATAAAGATATATAGTTTTATACTATTGTCA GGAGGATTTAAATTATCCTAAAAAGTCATTGATACAATGCTGCAACTATCAAGGATGTTT TTGGAAAGAATGCCCTCCACCTTGTTTACTCCTGAGAAAAGAAAGGAATGTTAAATTGGC TTATTCATAAAGGAGTGGATCTGTTGGAGAAAGACAAAGAGTCTGGATGGACAGCATTGC ACAGAAACATTTTTTATGGACATATTGAAAGCGTTTGGTATCTATTGAAGCATGGTGGTA GTCTGTATATTCAAGATAAAGAAGGCTTGTCAACTTTAGATCTTGTGATGAACGATAGAC CAACTCATGTAGTAATGAAGAATACTGATACTACAGATGTTTATACTTGGGGCGATAATA CAAATAATACCCTGCGTCATGGGAAGCCACATAGCAAACATCATGCAGAGTTGGTGGATC TGTTCTGCAGCAGTGAGATCATATCAAGCAGTTGCGCTTTGTAATTTCACTCAGTGTTCT GTCTTAAAAAGGCAGTTATACCTGGGTAATGGACCGAAAGCAATTAGACATGAAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_015525 |
| Insert Size | 5000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015525.2, NP_056340.2 |
| RefSeq Size | 5775 bp |
| RefSeq ORF | 4062 bp |
| Locus ID | 25998 |
| UniProt ID | Q9P2D0 |
| Protein Families | Druggable Genome |
| Gene Summary | Bruton tyrosine kinase (BTK) is a protein tyrosine kinase that is expressed in B cells, macrophages, and neutrophils. The protein encoded by this gene binds to BTK and downregulates BTK's kinase activity. In addition, the encoded protein disrupts BTK-mediated calcium mobilization and negatively regulates the activation of nuclear factor-kappa-B-driven transcription. This gene has a pseudogene on chromosome 18. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218657 | IBTK (Myc-DDK-tagged)-Human inhibitor of Bruton agammaglobulinemia tyrosine kinase (IBTK) |
CNY 13888.00 |
|
| RC218657L1 | Lenti ORF clone of Human inhibitor of Bruton agammaglobulinemia tyrosine kinase (IBTK), Myc-DDK-tagged |
CNY 16288.00 |
|
| RC218657L2 | Lenti ORF clone of Human inhibitor of Bruton agammaglobulinemia tyrosine kinase (IBTK), mGFP tagged |
CNY 16288.00 |
|
| RC218657L3 | Lenti ORF clone of Human inhibitor of Bruton agammaglobulinemia tyrosine kinase (IBTK), Myc-DDK-tagged |
CNY 16288.00 |
|
| RC218657L4 | Lenti ORF clone of Human inhibitor of Bruton agammaglobulinemia tyrosine kinase (IBTK), mGFP tagged |
CNY 7600.00 |
|
| RG218657 | IBTK (tGFP-tagged) - Human inhibitor of Bruton agammaglobulinemia tyrosine kinase (IBTK) |
CNY 15488.00 |
