TBC1D9B (BD127777) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BD127777, the custom clone sequence may differ by one or more nucleotides TACAACACCAGGGTGGTGGGAGCCCTGGTGGACCAAGGCATCTTCGAAGAGCTCACGAGAGACTTCCTGC CGCAGCTCTCGGAGAAGATGCAGGACCTGGGGGTGATCTCCAGCATCTCGCTGTCCTGGTTCCTGACCCT CTTCCTCAGCGTCATGCCCTTCGAGAGCGCCGTGGTCATCGTCGACTGCTTTTTCTATGAGGGCATCAAG GTGATCCTGCAGGTGGCCCTGGCCGTCCTGGACGCCAACATGGAGCAGCTGCTGGGCTGCAGCGACGAGG GCGAGGCCATGACCATGCTGGGCAGATACCTGGATAATGTGGTCAACAAGCAGAGTGTCTCTCCTCCTAT CCCGCACCTCCGTGCCTTGCTGAGCAGCAGCGATGACCCCCCTGCAGAGGTGGACATCTTTGAGCTCCTG AAAGTGTCCTATGAGAAATTCAGCAGCCTGAGGGCCGAAGACATTGAGCAGATGCGGTTTAAACAGAGGC TGAAAGTGATCCGGTCCTTGGAGGACACGGCCAAGAGGAGTGTGGTCCGAGCTATACCTGTGGACATTGG TTTCTCCATTGAAGAGCTGGAGGACCTTTACATGGTGTTTAAGGCCAAGCACCTGGCTAGCCAGTACTGG GGGTGCAGCCGCACAATGGCCGGCCGTCGGGACCCCAGCCTGCCCTACCTGGAGCAGTACCGGATTGATG CCAGCCAGTTCCGGGAACTCTTTGCCAGCCTGACACCCTGGGCCTGTGGCTCCCACACACCTCTGCTGGC AGGGCGCATGTTCAGGCTCCTGGACGAAAACAAGGACTCGCTGATCAACTTCAAGGAGTTCGTGACAGGG ATGAGCGGGATGTACCACGGGGACCTGACAGAGAAGCTCAAGGTGCTCTACAAGCTACACCTTCCCCCAG CTCTGAGCCCAGAGGAAGCCGAGTCAGCCCTGGAGGCGGCCCATTATTTCACAGAGGACAGCTCCTCAGA AGAAGCACTACCACAGGAAGAGCAAGAAGGAAGTGGAAGTGAGGAGAGAGGAGAGGAGAAGGGGACCAGC TCTCCGGACTATCGGCACTACCTTCGAATGTGGGCCAAGGAGAAAGAGGCTCAGAAGGAGACGATTAAGG ATCTTCCCAAGATGAACCAGGAGCAGTTCATTGAGCTGTGCAAGACGCTTTACAACATGTTCAGTGAAGA CCCCATGGAGCAGGACCTGTACCACGCCATCGCCACCGTGGCCAGCCTCCTGCTCCGCATCGGAGAGGTG GGGAAGAAGTTCTCAGCCCGCACAGGCAGGAAGCCCAGGGACTGTGCCACTGAGGAGGACGAGCCACCAG CACCCGAACTGCATCAGGACGCAGCCAGGGAGCTTCAGCCCCCAGCTGCAGGAGACCCCCAAGCCAAAGC AGGCGGAGACACACACCTCGGAACAGCCCCACAGGAGAGCCAGGTGGTGGTGGAGGGGGGCAGCGGCGAG GGACAGGGCTCACCCTCCCAGCTGCTGTCTGACGATGAAACCAAAGACGACATGTCCATGTCCTCCTACT CGGTGGTCAGCACGGGCTCCCTGCAATGTGAAGACCTTGCAGACGACACGGTGCTGGTGGGCGGGGAGGC CTGCAGCCCCACAGCGCGCATCGGCGGCACCGTCGACACCGACTGGTGCATCTCCTTTGAGCAGATCCTG GCCTCCATCCTGACGGAGTCCGTGCTGGTGAACTTCTTTGAGAAGAGAGTGGACATTGGACTCAAGATCA AGGACCAAAAGAAAGTGGAGAGACAGTTCAGCACCGCCAGTGACCATGAGCAGCCTGGAGTTTCCGGCTG ATGCCTGCAGCTGTGAGGCCTGGCCCAAGGTGTCATCAGTGGGGCTGGCCTCATCTCCTCCTGCCTTTCC TCCCTTATCAGTTTCTCTTTAAAGGTGTGCCCCTCCTGCTCTCCCAGGAGCAGTGAGTTGTGAGTGGAAA GAAGGCTGGTGCAGACCCAGCTGCCTTAGACAGATTCCCTGGGCCTGCATCTCCTGGCGCCGGCTGCTTC TGGGCCCAGGAAGAGGCTGTGGCTCCCACCTTCCTTACACCTGGTGGGAGCCCGCCTCGCACCAGCTGCA CCTGCCTAGCATTAGAGGCTCTCAGATCTGCCCTTGCTTGCCTCATACCTCTGTGCTCCACACTGCGGCC AGGCCAGCTGAGTCCCTCCATCCGTGGATGCTTTCCTGCAGCTATGTGGTATGGGGGTCATTCCTGCCTC TTGGCACCAGGTTGGGGGGCATGTGCTTGTTGGGCACCAAAGTGATGGAACCCTCAGGTGCTCTCCGGGA GCCTGAACCTCCTGACTGAGGAACATGGGCAGAACATGTTTATTGCACAGAGTGGGCGCTGCGCACAGGC GTGGCTGTACACGTGCTCTCAGCTCATCATCCTTTCCAGTAACTTTAAAAAAACATCCCTCAGGTCCTGA TATATTTCCTTGGATTCATTTCACTTGGCTAGAAATTACACTGTGCTCAATGCCTTAATAAATCCCTG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BD127777 unedited CCTTCTATAGGGCGGCCGCGAATTCGGCACGAGGGCCCTGTGCGAGCGCATGCTGCCCGA CTACTACAACACCAGGGTGGTGGGAGCCCTGGTGGACCAAGGCATCTTCGAAGAGCTCAC GAGAGACTTCCTGCCGCAGCTCTCGGAGAAGATGCAGGACCTGGGGGTGATCTCCAGCAT CTCGCTGTCCTGGTTCCTGACCCTCTTCCTCAGCGTCATGCCCTTCGAGAGCGCCGTGGT CATCGTCGACTGCTTTTTCTATGAGGGCATCAAGGTGATCCTGCAGGTGGCCCTGGCCGT CCTGGACGCCAACATGGAGCAGCTGCTGGGCTGCAGCGACGAGGGCGAGGCCATGACCAT GCTGGGCAGATACCTGGATAATGTGGTCAACAAGCAGAGTGTCTCTCCTCCTATCCCGCA CCTCCGTGCCTTGCTGAGCAGCAGCGATGACCCCCCTGCAGAGGTGGACATCTTTGAGCT CCTGAAAGTGTCCTATGAGAAATTCAGCAGCCTGAGGGCCGAAGACATTGAGCAGATGCG GTTTANACAGAGGCTGANAGTGATCCAGTCCTTGGAGGACACNGCCAAGAGGAGTGTGGT CCGAGCTATACCTGTGGACATTGGTTTCTCCATTNGAGAGCTGNANGACCTTNACATGGT GTTAAGGCCCAGCACCTGNCTAGCCAGTACTGGNGGTGCAGNCGCACAATGGCCCGGCGT CGGGACCCCAGCCTGCCCTACCTGGAGCAGTACCGGATTGATGCCAGNCCATTCCNGGNA ACTCTTTGCCAGCCTGACACCCTGGGCCCCTGTGCTCCNACACACCTCTGCTGGCAGGGC GCATGNTCAGGCTCCTGGNACGAAACAGACTCGCTGATCACTTTCAGGANTCNTGACAAG GATGACCGGGATTACCCACGGGACCTGACGAGAAAGCTCAGGTGCTCTACAAGCTCCCTT NCCCACTCTGAGCCANAGGAAGCGATCANCCTGGNAGCGCCATATT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BD127777 unedited GGCCGCAATCTAGAGTCGAGNTTTTTTTTTTTTTTTTTTTTTTTATTTCTTTCAGGGATT TATTAAGGCATTGAGCACAGTGTAATTTCTAGCCAAGTGAAATGAATCCAAGGAAATATA TCAGGACCTGAGGGATGTTTTTTTAAAGTTACTGGAAAGGATGATGAGCTGAGAGCACGT GTACAGCCACGCCTGTGCGCAGCGCCCACTCTGTGCAATAAACATGTTCTGCCCATGTTC CTCAGTCAGGAGGTTCAGGCTCCCGGAGAGCACCTGAGGGTTCCATCACTTTGGTGCCCA ACAAGCACATGCCCCCCAACCTGGTGCCAAGAGGCAGGAATGACCCCCATACCACATAGC TGCAGGAAAGCATCCACGGATGGAGGGACTCAGCTGGCCTGGCCGCAGTGTGGAGCACAG AGGTATGAGGCAAGCAAGGGCAGATCTGAGAGCCTGTAATGCTAGGCAGGTGCACCTGGT GCGAGGCGGGCTCCCACCAGGTGTAAGGAAGGTGGGGAGCCACAGCCTCTTCCTGGGCCC AGAAGCAGCCCGGGCCAGGAGATGCAGCGCCAGGGAATCTGTTTAGGCAGCTGGGCCTGC ACCAACCTTCTTTCCCCTCACAACTCACTGCTCCTGGGAGAACAGGAGGGGCCCACCCTT AAAGAGAAACCTGTCAAGGGACGAAAGGCCAGAGGGAAATACGCCCCCCCACCTGTGACC CCTTGGGCCAGGCCTTCAAGGTGCAAGGCTCAGCCCGGAAACTCCAGCTTGCTCCTGGCC CCTTGGGGGGCTGACTGGCTCTTCACTTTCTCTTGGCCCTGGACTTTAGCCCACAGGCCA CTTTTTTTTCAAAAAGTTTCCCCACCGGGCCCTCCCCGGATGGAGGCCCAGATCTTCCTC AAGGCAAACCTCATTCCGTTCCCCACGTCCCCCCTTACCCCCTTGGGGGCCGCCGCCT
Restriction Sites	NotI-NotI
ACCN	BD127777
Insert Size	2620 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BD127777.1
RefSeq Size	2518 bp
RefSeq ORF	2518 bp
Locus ID	23061
Domains	EFh
Gene Summary	May act as a GTPase-activating protein for Rab family protein(s).[UniProtKB/Swiss-Prot Function]

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