THRAP5 (MED16) (NM_005481) Human Untagged Clone
CAT＃: SC127046
MED16 (untagged)-Human mediator complex subunit 16 (MED16)
CNY 6432.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DRIP92; THRAP5; TRAP95 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_005481, the custom clone sequence may differ by one or more nucleotides
ATGTGTGATTTGCGGCGGCCAGCGGCAGGTGGGATGATGGACTTGGCCTACGTCTGTGAGTGGGAGAAAT GGTCCAAGAGCACCCACTGCCCATCGGTGCCCCTGGCCTGCGCCTGGTCCTGCCGAAATCTCATCGCCTT CACCATGGACCTGCGCAGCGATGACCAGGACCTGACCCGCATGATCCACATCCTGGACACGGAGCACCCC TGGGACCTGCACTCGATCCCCTCAGAGCACCACGAGGCCATCACCTGCCTGGAGTGGGACCAGTCAGGCT CCCGGCTCCTGTCAGCAGATGCCGACGGGCAGATCAAGTGCTGGAGCATGGCGGACCACCTGGCTAATAG CTGGGAGAGCTCAGTGGGCAGCCTAGTGGAGGGGGACCCCATTGTGGCCCTGTCCTGGCTGCACAATGGT GTGAAACTGGCCCTGCACGTGGAGAAGTCGGGCGCCTCCAGCTTCGGGGAGAAGTTCTCCCGAGTCAAGT TCTCACCGTCGCTCACGCTGTTCGGCGGCAAGCCCATGGAGGGCTGGATCGCGGTGACGGTCAGCGGCCT GGTCACCGTGTCCCTGCTGAAGCCCAGCGGGCAGGTGCTGACGTCCACCGAGAGCCTGTGCCGGCTGCGC GGCCGCGTGGCCCTGGCCGACATCGCCTTCACCGGCGGCGGCAACATCGTGGTGGCCACGGCGGACGGCA GCAGCGCGTCGCCCGTGCAGTTCTACAAGGTGTGCGTGAGCGTGGTGAGCGAGAAGTGCCGTATCGACAC GGAGATCCTGCCCTCCCTGTTCATGCGCTGCACCACCGACCTCAACCGCAAGGACAAGTTTCCCGCCATC ACCCACCTCAAGTTCCTGGCCCGGGACATGTCGGAGCAGGTGCTTTTGTGCGCGTCCAGCCAGACCAGCA GCATCGTGGAGTGCTGGTCCCTGCGCAAGGAGGGACTCCCCGTGAACAACATCTTCCAGCAGATCTCCCC CGTGGTTGGCGACAAACAGCCCACAATTCTCAAATGGCGGATCCTATCGGCCACCAACGATCTGGACCGT GTGTCGGCCGTGGCGCTGCCCAAGCTGCCCATCTCGCTCACCAACACCGACCTCAAGGTGGCCAGCGACA CACAGTTCTACCCTGGCCTCGGGCTGGCCCTGGCCTTCCACGACGGCAGCGTCCACATCGTGCACCGGCT CTCACTGCAGACCATGGCCGTCTTCTACAGCTCCGCGGCCCCGAGGCCTGTGGATGAGCCGGCCATGAAG CGCCCCCGCACCGCGGGCCCCGCCGTCCACTTAAAGGCTATGCAGCTATCGTGGACGTCACTGGCCCTGG TGGGGATTGACAGCCACGGGAAGCTGAGCGTGCTCCGCCTCTCACCTTCCATGGGCCACCCGCTGGAGGT GGGGCTGGCGCTGCGGCACCTGCTCTTCCTGCTGGAGTACTGCATGGTGACCGGCTACGACTGGTGGGAC ATCCTGCTGCACGTGCAGCCCAGTATGGTACAGAGCCTGGTGGAGAAGCTGCACGAGGAGTACACGCGCC AGACCGCTGCCCTGCAGCAGGTCCTCTCCACCCGGATCCTGGCCATGAAGGCCTCGCTCTGCAAGCTGTC GCCCTGCACGGTGACCCGCGTGTGCGACTACCACACCAAGCTCTTCCTCATCGCCATCAGCTCCACCCTG AAGTCGCTGCTGCGCCCCCACTTTCTCAACACGCCTGACAAGAGCCCCGGCGACCGGCTGACCGAGATCT GCACCAAGATCACCGACGTCGACATTGACAAGGTCATGATCAACCTCAAGACGGAGGAATTTGTGCTGGA CATGAACACACTGCAGGCGCTGCAGCAGCTCTTGCAGTGGGTGGGCGACTTCGTGCTGTACCTGCTGGCC AGCCTACCCAACCAGGGTTCCCTGCTGAGGCCGGGCCACAGCTTTCTGCGGGACGGCACCTCGCTGGGCA TGCTTCGGGAATTGATGGTGGTCATCCGCATCTGGGGCCTTCTGAAGCCCAGCTGCCTGCCCGTGTATAC GGCCACCTCGGATACCCAGGACAGCATGTCCCTGCTCTTCCGCCTGCTCACCAAGCTCTGGATCTGCTGT CGCGATGAGGGCCCAGCGAGCGAGCCGGACGAGGCGCTGGTGGATGAATGCTGCCTGCTGCCCAGCCAGC TGCTTATCCCCAGCCTGGACTGGCTGCCAGCCAGCGACGGCCTGGTTAGCCGCCTGCAGCCCAAGCAGCC CCTTCGTCTGCAGTTTGGCCGGGCGCCCACGCTGCCTGGCAGTGCTGCCACCCTGCAGCTCGACGGCCTC GCCAGGGCCCCAGGCCAGCCCAAGATCGACCACCTGCGGAGGCTGCACCTTGGCGCTTGCCCCACGGAGG AATGCAAGGCCTGCACCAGGTGCGGCTGTGTCACCATGCTCAAGTCGCCCAACAGAACCACGGCGGTGAA GCAGTGGGAGCAGCGCTGGATCAAGAACTGCCTGGCTGTTGAAGGCCGTGGGCCGGACGCCTGCGTGACC AGCAGAGCTTCTGAGGAAGCCCCTGCCTTTGTCCAGCTGGGCCCGCAGTCCACACACCACTCTCCCAGGA CCCCCAGATCCCTGGACCATCTGCATCCAGAGGACCGTCCGTGA >OriGene 5' read for NM_005481 unedited
CTCTTTATAGGGCGGCCGCGAATTCGACACGAGGTCGCACCGCGGACGCCCGCTGAGCTT GGCGCACGGGCCGACCAGGAGCTGGTGACTGCCCTCATGTGTGATTTGCGGCGGCCAGCG GCAGGTGGGATGATGGACTTGGCCTACGTCTGTGAGTGGGAGAAATGGTCCAAGAGCACC CACTGCCCATCGGTGCCCCTGGCCTGCGCCTGGTCCTGCCGAAATCTCATCGCCTTCACC ATGGACCTGCGCAGCGATGACCAGGACCTGACCCGCATGATCCACATCCTGGACACGGAG CACCCCTGGGACCTGCACTCGATCCCCTCAGAGCACCACGAGGCCATCACCTGCCTGGAG TGGGACCAGTCAGGCTCCCGGCTCCTGTCAGCAGATGCCGACGGGCAGATCAAGTGCTGG AGCATGGCGGACCACCTGGCTAATAGCTGGGAGAGCTCAGTGGGCAGCCTAGTGGAGGGG GACCCCATTGTGGCCCTGTCCTGGCTGCACAATGGTGTGAAACTGGCCCTGCACGTGGAG AAGTCGGGCGCCTCCAGCTTCGGAGAGAAGTTCTCCCGAGTCAAGTTCTCACCGTCGCTC ACGCTTNGTCGCGGGCAGCCCATGGAGGGCTGGATCGNCGTGACGGTCAGCGGCCTGGTC ACCGTGTCCCTGCTGAAGCCCAGCGGGCAGGTGCTGACGTCCACCCGAGAGCCTGTGCCN GCTGCGCGGNCGNGTGGCCCTGGCCGCATCGCCTTCACCGGGGNNGNNACATCGTGGTGG CCACGGNGGACGGANCAACGCGTCNCCGTGCATTTCTACAAGTGTGCCTGAACGTTGTGA CCAAAATTGCCTNTCCACACGGAGACCTGCCTTCCTGTTATGCGCTGACACCGACTTAAC GNAGAAAGTTCCCGCCATACCACTTAGTTCTGCCCGNAATGTNGAAAGTGCTTTGGCCCG TCCCCAACCACATGGGGTGCTGCCCTGC >OriGene 3' read for NM_005481 unedited
TGTACCGCGGGCCGCAATCTAGAGTCGAGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTGGGCGCAAAGGGGGTTTATTGGACCTGTCCTTTCCAACCGCTGGTT GTCCAAGTTTAACGCTTTTCGGGGGTGAGGGAAGGAAACCCAGGAGACCCCCCAACCGGG TCACCACAAAGGTCCCCTGGACCCCCGGGCGGCACGGACGGGCCTTTGGATGCAAATGGG CCAAGGATTTGGGGGTCCTGGGAAAATGGTGTGTGGACTGGGGGCCCAACTGGACAAAAG GAGGGGCTTTCTTAAAAACTTTTGTGGTCACGCAAGGGTTCGGGCCAAGGGCTTTAACAA CCCTGGAGGGGGGGCTGAGGTTAACCGCGCCCAAGAAAATTCAAGGCGAACGGTGGCTTT TGCAACAACCGGGCTTAAGGGCAACTGAGGGGGAACTGAGGAATAACTGAAGGGCAACTG GGCTTAAGGGTAACTTAGGGGCACCCCCCACCAAAACCCACCGGACAGGCAGTTTTTGAT TCAACGCTGGTTCCACTGGTTTAACGGCGTGGGTTTGTTGGGCGAATTGAGCATGGTGAC ACAAACGCAACTGGTGCAAGCCTTGTATTCCTTCGTGGGGGAAGCGCCAAGGTGCAGCCT TCGCAAGTGGGCCAATCTGGGCTTGGCTGGGGGCCTGGCGAGGCCGTCTAACTTCAAGGT GGCAACACTGGCAAGCCAACGTGTGCGCCCGGCCAAATTGCAAACAAAGGGCTTGTTGGG CTTGAGCGGCTATCCAGCCCGTTCTTGGTGGGACCACCCCAGCTTGGGATAACACNTGGG TGGGCACAAGTAAAATTTTTCACCACGGCTTTTCGGGTTGTTCGTTGGCGCTCATGGGAA AAAGACCCAAACTTGTGAGACAGCGAN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005481 |
| Insert Size | 3060 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005481.2, NP_005472.2 |
| RefSeq Size | 2952 bp |
| RefSeq ORF | 2634 bp |
| Locus ID | 10025 |
| UniProt ID | Q9Y2X0 |
| Domains | WD40 |
| Protein Families | Druggable Genome, Transcription Factors |
| Gene Summary | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218942 | MED16 (Myc-DDK-tagged)-Human mediator complex subunit 16 (MED16) |
CNY 6424.00 |
|
| RC218942L3 | Lenti-ORF clone of MED16 (Myc-DDK-tagged)-Human mediator complex subunit 16 (MED16) |
CNY 9030.00 |
|
| RC218942L4 | Lenti-ORF clone of MED16 (mGFP-tagged)-Human mediator complex subunit 16 (MED16) |
CNY 9030.00 |
|
| RG218942 | MED16 (tGFP-tagged) - Human mediator complex subunit 16 (MED16) |
CNY 7890.00 |
