Kaiso (ZBTB33) (NM_006777) Human Untagged Clone
CAT＃: SC126900
ZBTB33 (untagged)-Human zinc finger and BTB domain containing 33 (ZBTB33), transcript variant 2
CNY 4928.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ZNF-kaiso; ZNF348 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC126900 sequence for NM_006777 edited (data generated by NextGen Sequencing)
ATGGAGAGTAGAAAACTGATTTCTGCTACAGACATTCAGTACTCTGGCAGTCTGCTGAAC TCCTTGAATGAGCAACGTGGCCATGGACTCTTCTGTGATGTTACCGTTATTGTGGAAGAC CGAAAATTCCGGGCTCACAAGAATATTCTTTCAGCTTCTAGTACCTACTTCCATCAGCTC TTCTCTGTTGCTGGGCAAGTTGTTGAACTGAGCTTTATAAGAGCAGAGATCTTTGCAGAA ATTCTCAATTATATCTATAGTTCTAAAATTGTTCGTGTTAGATCAGATTTGCTTGATGAG TTAATTAAATCAGGGCAGTTATTAGGAGTGAAATTTATAGCAGAGCTTGGTGTCCCATTG TCACAGGTTAAAAGCATCTCAGGTACAGCGCAGGATGGTAATACTGAGCCTTTACCTCCT GATTCTGGTGACAAGAACCTTGTAATACAGAAATCAAAAGATGAAGCCCAAGATAATGGG GCTACTATAATGCCTATTATAACAGAGTCTTTTTCATTATCTGCCGAAGATTATGAAATG AAAAAGATCATTGTTACCGATTCTGATGATGATGATGATGATGTCATTTTTTGCTCCGAG ATTCTGCCCACAAAGGAGACTTTGCCGAGTAATAACACAGTGGCACAGGTCCAATCTAAC CCAGGCCCTGTTGCTATTTCAGATGTTGCACCTAGTGCTAGCAATAACTCGCCCCCTTTA ACAAATATCACACCTACTCAGAAACTTCCTACTCCTGTGAATCAGGCAACTTTGAGCCAA ACACAAGGAAGTGAAAAATTGTTGGTATCTTCAGCTCCAACACATCTGACTCCCAATATT ATTTTGTTAAATCAGACACCACTTTCTACACCACCAAATGTCAGTTCTTCACTTCCAAAT CATATGCCCTCTTCAATCAATTTACTTGTGCAGAATCAGCAGACACCAAACAGTGCTATT TTAACAGGAAACAAGGCCAATGAAGAGGAGGAGGAGGAAATAATAGATGATGATGATGAC ACTATTAGCTCCAGTCCTGACTCGGCCGTCAGTAATACATCTTTGGTCCCACAGGCTGAT ACCTCCCAAAATACCAGTTTTGATGGATCATTAATACAGAAGATGCAGATTCCTACACTT CTTCAAGAACCACTTTCCAATTCCTTAAAAATTTCAGATATAATTACTAGAAATACTAAT GATCCAGGCGTAGGATCAAAACATCTAATGGAGGGTCAGAAGATCATTACTTTAGATACA GCTACTGAAATTGAAGGCTTATCGACTGGTTGCAAGGTTTATGCAAATATCGGTGAAGAT ACTTATGATATAGTGATCCCTGTCAAAGATGACCCTGATGAAGGGGAGGCCAGACTTGAG AATGAAATACCAAAAACGTCTGGCAGCGAGATGGCAAACAAACGTATGAAAGTAAAACAT GATGATCACTATGAGTTAATAGTAGATGGAAGGGTCTATTATATCTGTATTGTATGCAAA AGGTCATATGTCTGTCTGACAAGCTTGCGGAGACATTTTAACATTCATTCTTGGGAGAAG AAGTATCCGTGCCGTTACTGTGAGAAGGTATTTCCTCTTGCAGAATATCGCACAAAGCAT GAAATTCATCACACAGGGGAGCGAAGGTATCAGTGTTTGGCCTGTGGCAAATCTTTCATC AACTATCAGTTTATGTCTTCACATATAAAGTCAGTTCATAGTCAAGATCCTTCTGGGGAC TCAAAGCTTTATCGTTTACATCCATGCAGGTCTTTACAAATCAGACAATATGCATATCTT TCCGATAGATCAAGCACTATTCCTGCAATGAAGGATGATGGTATTGGGTATAAGGTTGAC ACTGGAAAAGAACCTCCAGTAGGGACCACTACATCTACTCAGAACAAGCCAATGACCTGG GAAGATATTTTTATTCAGCAGGAAAATGATTCAATTTTTAAACAAAATGTAACAGATGGC AGTACTGAGTTTGAATTTATAATACCAGAGTCTTACTAA Clone variation with respect to NM_006777.3 >OriGene 5' read for NM_006777 unedited
GAATTCGGCACCAGGCGGCGGCCGCGTCGACGTCGACCCAGACTGGAGCGACGTTTAAAG AAGGGGCAGAATCGCTGGGGAGTGCGGCTTCTTCTTGTTGGGGGACTCCCAGCCTTCCGC GCGTCCGGAGGAGGAGAAGCGGCGGCGCCGGGAAGCAGGCATGGAGAGTANAAAACTGAT TTCTGCTACAGACATTCAGTACTCTGGCAGTCTGCTGAACTCCTTGAATGAGCAACGTGG CCATGGACTCTTCTGTGATGTTACCGCTATTGTGGAAGACCGAAAATTCCGGGCTCACAA GAATATTCTTTCAGCTTCTAGTACCTACTTCCATCAGCTCTTCTCTGTTGCTGGGCAAGT TGTTGAACTGAGCTTTATAAGAGCAGAGATCTTTGCAGAAATTCTCAATTATATCTATAG TTCTAAAATTGTTCGTGTTAGATCACATTTGCTTGATGAGTTAATTAAATCACGGCAGTT ATTACGAGTGAAATTTATACCAGAGCTTGGTGTCCCATTGGCACAGGTTAAAAGCATTTT AGGTCCAGCGCAGGATGGTAATACTGAGCCTTTACCCTCTGATCCTGGTGACAAGAACCT TGTCACCCCAAATTAAAACATGAACCCCCAAATAACGGGGGCTCCTATAATGCCCTATAC ACCACAGTTTTTCTCCTTACCTGGCCAACATCATGAAATCGAACACCCCACTTGTCCCCC CCTCCTACGATCATTACGAAGATTGCCCTTTCTCCGCCCCCACATCTTCACCACC >OriGene 3' read for NM_006777 unedited
CCCCCTTGAGTNTGACCGGCGAGCCGCATTTACGACCGGGTTTTTTCTTTTTTTTTTTTT TTCAGAAATTAATCATTTTATTTTTGCCACAGAAAGCTCTAAGAACTAATATCAGGCCAT GGTTAATGAAAAGTAGAACTTACAGTCTTGACAGGGAAAAATTGTACAAGTGTGAACATT ACAGTACTCGGAGGAATGAACAGGGTTTCATATAGTTGACCAGCCAAAAAGCCAGTGCTG TTAAGTTAGCATTTAATTTTTTAGAATATGCATGCCTTGACAATACAAAATACCTTGATC AGTGTTTACCATTTTTTTATCTTTTTGGCACAATGGGGGAAAAATGCGGCATTATTTCTT CATAGTGGTTGTGTGTGGGGGATCGTTACCACCAAAATCATAATAATCATTTAAGGTATA TTTTAAAGTTAGTCTCGCAGGATTGTTATGGCATTTTTAAATTTCTGTAGTTTAAGCATT TTCATTTTGCCATATCTTTTACCAAATTACTATTTCCAGTAGTTCCTATCCAGACCCTTT ACCGGTAATTTAGCATAGGAGCAAATTTGAAGCTACCTTCCCACAACAAACGGAAGTTTA ATTCGCCGTAGCTTACGCATCCCATAAAAACACACTTATGGCAATTTTAAACAGTTCTCT TGCTACAGAATTTCTCATTCCATACATACCCTCTAATGGAGGACTTTTTGCCCCAACAGA CCCCTTCCTCATAAAACTTCTACCTTTGCCCTCTGCAACCTTACACCACCAACTTTAGCC CCCTTACCTCATGGCCTTCCCTGGAGGTCCCCTGTTCAACCCACTATCTCTTACTCCAGT CACTAAAATCATCTCCCCCTCTCAATCCACCTTCTTTTTCTTCACCCCTTTTTCCTCCTC TAATCCCCCCTTATAATACTCCTCTATCTCTATTCCCATCTCACC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_006777 |
| Insert Size | 4840 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006777.3, NP_006768.1 |
| RefSeq Size | 5225 bp |
| RefSeq ORF | 2019 bp |
| Locus ID | 10009 |
| UniProt ID | Q86T24 |
| Domains | BTB, zf-C2H2 |
| Gene Summary | This gene encodes a transcriptional regulator with bimodal DNA-binding specificity, which binds to methylated CGCG and also to the non-methylated consensus KAISO-binding site TCCTGCNA. The protein contains an N-terminal POZ/BTB domain and 3 C-terminal zinc finger motifs. It recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. It may contribute to the repression of target genes of the Wnt signaling pathway, and may also activate transcription of a subset of target genes by the recruitment of catenin delta-2 (CTNND2). Its interaction with catenin delta-1 (CTNND1) inhibits binding to both methylated and non-methylated DNA. It also interacts directly with the nuclear import receptor Importin-α2 (also known as karyopherin alpha2 or RAG cohort 1), which may mediate nuclear import of this protein. Alternatively spliced transcript variants encoding the same protein have been identified.[provided by RefSeq, May 2010] Transcript Variant: This variant (2) lacks an exon in the 5' UTR, as compared to variant 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207287 | ZBTB33 (Myc-DDK-tagged)-Human zinc finger and BTB domain containing 33 (ZBTB33), transcript variant 2 |
CNY 4928.00 |
|
| RC207287L1 | Lenti ORF clone of Human zinc finger and BTB domain containing 33 (ZBTB33), transcript variant 2, Myc-DDK-tagged |
CNY 7328.00 |
|
| RC207287L2 | Lenti ORF clone of Human zinc finger and BTB domain containing 33 (ZBTB33), transcript variant 2, mGFP tagged |
CNY 5890.00 |
|
| RC207287L3 | Lenti ORF clone of Human zinc finger and BTB domain containing 33 (ZBTB33), transcript variant 2, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC207287L4 | Lenti ORF clone of Human zinc finger and BTB domain containing 33 (ZBTB33), transcript variant 2, mGFP tagged |
CNY 5890.00 |
|
| RG207287 | ZBTB33 (tGFP-tagged) - Human zinc finger and BTB domain containing 33 (ZBTB33), transcript variant 2 |
CNY 4370.00 |
