HSPC210 (GSKIP) (AF151044) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C14orf129; HSPC210
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC126882 sequence for AF151044 edited (data generated by NextGen Sequencing) ATGGAAACAGACTGTAATCCCATGGAGCTAAGCAGTATGTCAGGATTTGAAGAAGGTTCA GAGCTGAACGGTTTTGAAGGAACTGACATGAAAGACATGAGGCTCGAAGCTGAAGCAGTT GTAAATGATGTTCTCTTTGCTGTTAACAACATGTTTGTCTCGAAAAGCCTGCGGTGTGCG GATGATGTGGCCTATATCAATGTGGAAACAAAGGAAAGAAACAGATATTGCCTAGAACTC ACTGAAGCAGGGCTCAAGGTGGTAGGCTATGCTTTTGACCAGGTAGATGATCATTTACAG ACTCCCTACCATGAAACAGTCTACTCCTTGTTGGATACACTCAGCCCCGCCTACCGAGAA GCATTTGGAAACGCACTGCTTCAAAGACTGGAAAGCTTTGAAAAGAGATGGACAGTCATG ACTACACTTTTTCCTTTCAGAGGGGCTGGTGCTGGTACAGAATGTTGA Clone variation with respect to AF151044.1 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AF151044 unedited AAAGATTAGGAATAAGAAAACCCGACTTCACTATNAGGGCGGCACGCGCAATTCGGCACG AGCGGGCGCGCATTTCGGTGCTGATTATCACAACTGTTTGGTGACCTACTTCACTGACCT GTGAGGATTCCTTCCCTTCAAAATGGAAACAGACTGTAATCCCATGGAGCTAAGCAGTAT GTCAGGATTTGAAGAAGGTTCAGAGCTGAACGGTTTTGAAGGAACTGACATGAAAGACAT GAGGCTCGAAGCTGAAGCAGTTGTAAATGATGTTCTCTTTGCTGTTAACAACATGTTTGT CTCGAAAAGCCTGCGGTGTGCGGATGATGTGGCCTATATCAATGTGGAAACAAAGGAAAG AAACAGATATTGCCTAGAACTCACTGAAGCAGGGCTCAAGGTGGTAGGCTATGCTTTTGA CCAGGTAGATGATCATTTACAGACTCCCTACCATGAAACAGTCTACTCCTTGTTGGATAC ACTCAGCCCCGCCTACCGAGAAGCATTTGGAAACGCACTGCTTCAAAGACTGGAAGCTTT GAAAAGAGATGGACAGTCATGACTACACTTTTTCCTTTCAGAGGGGCTGGTGCTGGTACA GAATGTTGATATAAAGCTTAAAATTCTTGCATATGGTCATAGAAAATGCATCTTTGGTTT TGTGTTTTTATCACTTGCTTCCAACTTAAGCTTTTGGCTCAGAAGATTATTGAATAATGA TTTGTCTTAGTTTCTGTTTCAGTAAGGGAATTCTGAGGCCGTTGCTATGATACCATCATT AAGACATTCACATGTCTTCATATAATATCTCTTCATTTCAAATCCTAATCACTATTTCAT ACTATTACAGGGCTNTGATGCTGCCAGCACTGTCTTTTACATAGGAAAAATCTAGATTGC CCAGTATAGAGGAATTAGAAGTACCCTACTATAC
Restriction Sites	NotI-NotI
ACCN	AF151044
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AF151044.1, AAF36130.1
RefSeq Size	1152 bp
RefSeq ORF	1152 bp
Locus ID	51527
Domains	DUF727
Gene Summary	This gene encodes a protein that is involved as a negative regulator of GSK3-beta in the Wnt signaling pathway. The encoded protein may play a role in the retinoic acid signaling pathway by regulating the functional interactions between GSK3-beta, beta-catenin and cyclin D1, and it regulates the beta-catenin/N-cadherin pool. The encoded protein contains a GSK3-beta interacting domain (GID) in its C-terminus, which is similar to the GID of Axin. The protein also contains an evolutionarily conserved RII-binding domain, which facilitates binding with protein kinase-A and GSK3-beta, enabling its role as an A-kinase anchoring protein. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2012]

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