KMT5C (BC005842) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FLJ98627; KMT5C; MGC2705; suppressor of variegation 4-20 homolog 2; suppressor of variegation 4-20 homolog 2 (Drosophila)
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC005842 edited AGCGGATGAGATCGTGGGGCTCACCAGCGTCCCCCATGGCTTCTGAGTAGCGTGGGAGTG GAGTCAGCACCAAGCCAGGCTCCCCGCGCCTGCCTTGCCCTCACCTGCTCCTGCTCTCTG CCAGAGGCAGCATGGTCCGCAGGGCACCATGGGGCCCGACAGAGTGACAGCACGAGAACT GTGCGAGAACGACGACCTGGCCACCAGCCTCGTCCTGGACCCCTACCTCGGTTTCCGCAC CCATAAGATGAACGTCAGGTCTATCGCTACCTCCGTGCCTTCCTGCCGGAAAGTGGCTTT ACCATCCTGCCCTGCACGCGCTACTCCATGGAGACCAACGGGGCCAAGATCGTGTCCACT CGTGCTTGGAAAAAGAATGAGAAGCTGGAGCTGCTGGTGGGCTGCATTGCAGAGCTGCGG GAGGCAGATGAGGGGCTGCTGAGGGCCGGTGAGAATGACTTCAGCATCATGTACTCAACC CGCAAGCGGAGTGCTCAGCTGTGGCTGGGCCCAGCCGCCTTCATCAACCATGACTGCAAA CCCAACTGCAAGTTTGTGCCTGCAGATGGGAACGCAGCCTGCGTGAAGGTGCTCCGGGAC ATTGAGCCTGGGGACGAGGTGACATGCTTCTACGGCGAGGGCTTCTTCGGCGAGAAGAAT GAGCACTGTGAATGCCACACCTGTGAGAGGAAAGGTGAAGGAGCTTTCCGAACCAGGCCT AGGGAGCCCGCGTTGCCACCACGGCCCCTGGACAAGTACCAGCTGCGTGAGACCAAGCGG CGGCTGCAGCAAGGCCTGGACAGTGGCAGCCGACAGGGCCTGCTGGGCCCTCGGGCCTGC GTGCACCCATCCCCGCTGCGCCGGGACCCATTCTGCGCCGCCTGCCAGCCCCTGCGCCTG CCAGCCTGCAGCGCCCGCCCAGACACCTCACCCCTCTGGCTCCAGTGGCTGCCTCAGCCC CAGCCCCGAGTGCGGCCCCGGAAGCGCCGACGCCCCCGGCCCCGGAGGGCCCCAGTGCTC TCCACCCACCACGCTGCCCGCGTCTCCCTGCACCGATGGGGAGGCTGTGGCCCCCACTGC CGCCTGCGAGGAGAGGCCCTGGTGGCCCTGGGCCAGCCCCCCCACGCCCGCTGGGCCCCT CAGCAGGACTGGCACTGGGCCCGGCGCTATGGGCTGCCTTACGTGGTGCGTGTGGACCTT CGTCGCCTGGCCCCAGCCCCACCAGCTACCCCAGCCCCTGCTGGGACCCCAGGCCCCATC CTGATCCCGAAGCAGGCCCTCGCCTTCGCCCCCTTCTCCCCACCCAAGCGCCTACGGCTG GTGGTCAGCCACGGCTCCATCGACCTGGATGTCGGCGGTGAAGAGCTGTGACAGGCCGGA CGGGGAGGCCCAGCAGGGAGAGAGGGTCTCTCTCCTAGCTGCTACCCAGGACCTCCAGAA GGAGCCCTTGGACCTCTGGGAGGGAGCTGACCCTTGACTCCAGCATAGCTCTGACCCTGG AATGGGGTTGGTTTGGACACCCCCAGGGATCTGAGCCCTGACCCTTTGTGACTGCTGACC CCTGAGCCACCCCCACTCCCACAGGGAGCCCCGGCCATTTGCTGCCCTCCCCACCCCTGC CCCAGCCTCAGGACTGCAGGAGCCATCCGCCCCCCTCAGCCCCTTCCTCCCCAGGGAGCA AAGCCATAAGGGGCAGGGGCCACCCCACGGCATCTCCCCAGAAGTACAGGCCTCAGGAGG AGGTGGAACTGATGTAGGGGGTGGCACTCCCCAGAGACTGCCCTCACGAGGGGACTGGGT TCGCTCTCAGCTCTGCAGCTGTCTGCGGTGGGGGGAAGGTTGGGGGGTGTCTGGAGGCAT GTTCCCCTCACCACCCCCCGTGGGTCTCAGGGAGGCCGGGTGTGACCTCATCTTTCTCAT GGTGCTATCCTGGTGCTATTGGGGTGGGGAGCTCCCTCCCCTCCCCCACACCAGAAAGGG GTATGTTGGGGGCTTGGAAGCACTTGAACTTTTTATTTTATTAAAACCTTGTTATAAGCA GCAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	NotI-NotI
ACCN	BC005842
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC005842.2, AAH05842.1
RefSeq Size	2067 bp
Locus ID	84787
Protein Families	Druggable Genome
Protein Pathways	Lysine degradation
Gene Summary	SUV420H2 and the related enzyme SUV420H1 (MIM 610881) function as histone methyltransferases that specifically trimethylate nucleosomal histone H4 (see MIM 602822) on lysine-20 (K20) (Schotta et al., 2004 [PubMed 15145825]).[supplied by OMIM, Dec 2009]

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