C10orf65 (BC057821) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	chromosome 10 open reading frame 65; DHDPS-like protein; Dihydrodipicolinate synthase-like, mitochondrial; FLJ37472; OTTHUMP00000059366
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC057821 edited ATTGCGGGAGGAGGGCCCAGATCCCACTGGAGATACCGAAGGAGATACTCTTTAGTATTG GGATCCCAGAAACCAGTCCTATATCTGGCCAGAGGGACACTGGGTTGTGGCATCTCTCTA GCTGCTACCCAGAAGGAACAGGGCCCCCTGGGGCCTATAGGCCTTGCCCCTGACCCTGGG AACACCCAGCTCAGGCCTGCCCCAGTGGCCACAAGTCAGGGAGGCCGCAAATTTTTAACT AGAAACATTGATCATTAATAGGGGGTTAGAAAGAGTTCAAACTAAGTCTCACTCTGGGAC ATAGACCAATTGTGCTTCAGGCCTCCTGCAGAGATGCTGGGTCCCCAAGTCTGGTCTTCT GTGAGGCAGGGGCTAAGCAGGAGCTTGTCCAGGAATGTGGGGGTCTGGGCCTCAGGGGAG GGGAAGAAGGTGGACATTGCGGGTATCTACCCCCCTGTGACCACCCCCTTCACTGCCACT GCAGAGGTGGACTATGGGAAACTGGAGGAGAATCTGCACAAACTGGGCACCTTCCCCTTC CGAGGAGCTGTGGGGGGCGTCTGCGCCCTGGCCAATGTCCTGGGGGCTCAGGTATGCCAG CTGGAGCGACTGTGCTGCACGGGGCAATGGGAAGATGCCCAGAAACTGCAGCACCGCCTC ATTGAGCCAAACGCTGCGGTGACCCGGCGCTTTGGGATCCCAGGGCTGAAGAAAATCATG GACTGGTTTGGCTACTATGGAGGCCCCTGCCGCGCCCCCTTGCAGGAGCTGAGCCCCGCT GAGGAGGAGGCACTGCGCATGGATTTCACCAGCAACGGCTGGCTCTGAGGGCAGGCAGGG TCCATGGCTGGCCTGAGCCCATCTCAGCCTCCTGCCTTGCACTTGCAGCCTGAAGCGGAG AGCACAGGGGGATGAGGGTGGCAGGCAGCGGGGAGCCGATAGAGGCTCCTTTGCCTGCTG TGGTCCTCCAGGCAGCCTTTCACAGGCACGCCCATGCATATCTCCTATTCTAACGGCCCC TGACCTCTCCCTTTTGGATCCTAAACTGTGTCTCTGGTCTGAAGACTGGGAAGGAGCAAT TTCTCAATTTATCTTTCTACTGTGGATGCTTTCCTACGCCCTGAGGCACATGAAGTCAGA AAGGAAGGGCAGAGGGGCAAGTAGGCACAGTAAGGGAATTTTCTTTTCTTTTTTTTTTTT TTGAGACAGAGTTTCACTCTGTCACCCAGGCTGGAGTGCAATGGCATGATCTCGCCTCCT GGGTTCAAGCGATTCTCCTGCATCAGCCTCCCGAGTAGCTGGGATTACAGGCGCCTGCCA CCACGCCCAGCTAATTTTTGTATTTTTAGCAGAGATGGGGTTTCACCGTGTTGGCCAGGC TGGTCTTGAACTCCTGACCTCAGGTGATCCAACTGCCTCGGCCTCCCAAAGTGCTGGGAT TACAGCTGTGAGCCACTGCACCCGGCCGGGAATTTATCAAAGGGGGGACTACTTAGATTT GGGGCTTCAAACCTGAGTCTAGCACAATGCAGCTGGAGGCCAGGGCTACTCCTACAAACC CAAGTTCTGACTCCTTCCAGGGAAGTATCAGCTTAAGAAACTTGATCAGGAAAGTGACAC TGGTCTTTCAAAGAGCCCTCTTTACCCTCCCATCCCTGCCCTCCACACTGCAAGCTGCAT TCCAGCCACAACGCCCTTTTCTAAATCTATTTTCATTCATCTCCTATTCGGTCTGTAGCC TTGATTCCAACCATTGAATGCACGGAGACAGCCCAGTGGCTTGTCAGATTGGTAAATAAT GACACCTGGTGGATAAATGTTACATTCTAGAAGACTATTCTGGTCAGCCTAGGTCTGAAA CACTAAGGAGACTTGGCCTGAGATAGGGCCATCCACTTGCCTACAGGCACTCTGCTTTTA TTATTAAAAATAGTCACTTTGTTACTATAATAAAATTTAACGAAAAAAAAAAAAAAAA
Restriction Sites	Please inquire
ACCN	BC057821
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC057821.1, AAH57821.1
RefSeq Size	1978 bp
Locus ID	112817
Gene Summary	The authors of PMID:20797690 cloned this gene while searching for genes in a region of chromosome 10 linked to primary hyperoxalurea type III. They noted that even though the encoded protein has been described as a mitochondrial dihydrodipicolinate synthase-like enzyme, it shares little homology with E. coli dihydrodipicolinate synthase (Dhdps), particularly in the putative substrate-binding region. Moreover, neither lysine biosynthesis nor sialic acid metabolism, for which Dhdps is responsible, occurs in vertebrate mitochondria. They propose that this gene encodes mitochondrial 4-hydroxyl-2-oxoglutarate aldolase (EC 4.1.3.16), which catalyzes the final step in the metabolic pathway of hydroxyproline, releasing glyoxylate and pyruvate. This gene is predominantly expressed in the liver and kidney, and mutations in this gene are found in patients with primary hyperoxalurea type III. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Nov 2010]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.