FLJ40125 (BC062452) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC062452 edited GGGGAGAGTTAGTGGAAGGGACTTAAGAGAAAGGGCGTGGTCTTTTGGAAAGGGGCGATT CTGGGTCGTAGGAGCCGGGCCGGAAGTATTGATTAGTGCTGGAAGGTGGAAGAGCAGGGC AGCCTGGACAACATGACCTGCATCCTGGTCTGCTTCCCTGGGGCCCCTAGGCCTTCTGAG GAGGCGATCAGGAGGGAGCTAGCACTGGACGCAGCCCTGGGCTGCAGAATCGCTGAACTG TGTGCCTCTGCTCAGAAGCCCCCCAGCCTGAACACAGTTTTCAGGACTCTGGCCTCAGAG GACATCCCAGATTTACCTCCTGGGGGAGGGCTGGACTGCAAGGCCACTGTCATTGCTGAA GTTTATTCTCAGATCTGCCAGGTCTCAGAAGAGTGCGGAGAGAAGGGGCAGGATGGGGCT GGGAAGTCCAACCCCACGCATTTGGGCTCAGCCTTGGACATGGAGGCCTGACAGCTGTTG TCCTTTGGGGATCCTTTGCTTCTCTGGGGCCTCAACAGAACTAAAGAAGAAAACCGACCC TTTCCCCAACTACATGTACCAGCGGAAGGAAGGAAGGCCAATGTAGGAACCCAAAATGCT TATTTCTTCTTCTCTTACTTCCCTCTCACAGAAAAGTCTTACGAATGGGGAAATTCCACC AACATCCAGACCAAAAAGAAAAAAGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC062452 unedited AGGTTCATATTTGTATACGACTCATATAGGCGGCCGCGTATTCGGCCATTACGGCCGGGG AGAGTTAGTGGAAGGGACTTAAGAGAAGGGCGTGGTCTTTTGGAAAGGGGCGATTCTGGG TCGTAGGAGCCGGGCCGGAAGTATTGATTAGTGCTGGAAGGTGGAAGAGCAGGGCAGCCT GGACAACATGACCTGCATCCTGGTCTGCTTCCCTGGGGCCCCTAGGCCTTCTGAGGAGGC GATCAGGAGGGAGCTAGCACTGGACGCAGCCCTGGGCTGCAGAATCGCTGAACTGTGTGC CTCTGCTCAGAAGCCCCCCAGCCTGAACACAGTTTTCAGGACTCTGGCCTCAGAGGACAT CCCAGATTTACCTCCTGGGGGAGGGCTGGACTGCAAGGCCACTGTCATTGCTGAAGTTTA TTCTCAGATCTGCCAGGTCTCANAAGAGTGCGGAGAGAAGGGGCAGGATGGGGCTGGGAA GTCCAACCCCACGCATTTGGGCTCAGCCTTGGACATGGAGGCCTGACAGCTGTTGTCCTT TGGGGATCCTTTGCTTCTCTGGGGCCTCAACAGAACTAAAGAAGAAAACCGACCCTTTCC CCAACTACATGTACCANCGGAAGGAAGGAAGGCCAATGTAGGAACCCAAAATGCTTATTT CTTCTTCTCTTACTTCCCTCTCACAGAAAAGTCTTACGAATGGGGAAATTCCACCAACAT CCAGACCAAAAAGGAAAAAGCCCANNNNANANNNNNAANNNNNAAAAAAAAAAATGGTTG GCCGCCTCGGCCCTCGCCTCTAGATTGCCGCCCGCGGTCATAGCTGTTTCCTGAACAGAT CCCGGGTGGCATCCCTGGGACCCCTTCCCAGTGCCCTCTCTTGCCCCTGGGAGTGGCCAC TCCGTGCCCCACCAACCTTTGTCCTAATAAAATAAAGTG
Restriction Sites	NotI-NotI
ACCN	BC062452
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC062452.1, AAH62452.1
RefSeq Size	719 bp
Locus ID	147699

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