TMEM158 (BC057390) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BBP; BINP receptor; brain injury-derived neurotrophic peptide (BINP) binding protein; brain specific binding protein; DKFZp586E1621; p40BBP; Ras-induced senescence 1; Ras induced senescence 1; RIS1; transmembrane protein 158
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC057390 edited CGGCAGCCCAAGGGCCCCGGCGCCGGGGGCGGCGGGGAACCCCAAACGCAACCGGGTCTG GAGGGATCCCCGCGCCGAGCCAGCCGCCGTCACCGCCTCCGCGCCGCCCCTGCGGGCTTG GCAGGCGCCCGGCGCGCCCGCACTGCGCCCGGCCGCCGGCTCCCGCGGTCCCACCGTGAG CTCGCCGGCCCGTCGCCCGCTCGCCATGCAACCGCCGCCGGCCTCGCGCGCGTAGGCGCC CGCCGCAGGCCATGCTGCCCCTGCTCGCCGCGCTCCTGGCCGCCGCCTGCCCGCTGCCGC CCGTCCGCGGCGGGGCCGCGGACGCGCCCGGCCTCCTCGGGGTGCCCTCCAATGCTTCAG TCAACGCGTCCTCCGCGGACGAGCCCATCGCCCCGCGGCTGCTGGCCTCGGCGGCCCCCG GGCCCCCCGAGCGCCCGGGCCCGGAGGAGGCGGCGGCGGCGGCGGCGCCGTGCAACATCA GCGTGCAGCGGCAGATGCTGAGCTCGCTGCTCGTGCGCTGGGGCCGCCCGCGGGGCTTCC AGTGCGACCTACTGCTCTTCTCCACCAACGCGCACGGCCGCGCTTTCTTCGCCGCCGCCT TCCACCGCGTCGGGCCGCCGCTGCTCATCGAGCACCTGGGGCTGGCGGCGGGCGGCGCGC AGCAGGACCTGCGCCTCTGCGTGGGCTGCGGCTGGGTGCGCGGTCGCCGCACCGGCCGCC TCCGGCCCGCCGCCGCCCCCAGCGCCGCCGCCGCCACCGCCGGGGCGCCCACCGCGCTGC CAGCCTACCCCGCGGCCGAGCCGCCCGGGCCGCTGTGGCTGCAGGGCGAGCCGCTGCATT TCTGCTGCCTAGACTTCAGCCTGGAGGAGCTGCAGGGCGAGCCGGGCTGGCGGCTGAACC GTAAGCCCATTGAGTCCACGCTGGTGGCCTGCTTCATGACCCTGGTCATCGTGGTGTGGA GCGTGGCCGCCCTCATCTGGCCGGTGCCCATCATCGCCGGCTTCCTGCCCAACGGCATGG AACAGCGCCGGACCACCGCCAGCACCACCGCAGCCACCCCCGCCGCTGCCGCCGCCGCCG CGGCCGTCACTTCGGGGGTGGCGACCAAGTGACCCGCTCCGCTCCTCCCTGTGTCCGTCC TGTGTCCGCGCGCGCGGGTGCCTTTCCCGCCGGAGACTCGGCCGGTGTGCTTCGTGCTGT AGTTATCGTTAGTTCCTCTTCCCGAGATGGGGCCGCCGAGAGACCCCAGCGCCTTTGAAA AGCAAGGTTTGTGCTGCGCTTCCAGTTCCGAAAAGCAGATGTTTAAGCCCTTGGACTGAG GGTGGGATCGCAGCTCCGAAGACGGAGAGGAGGGAAATGGGGCCCTTTCCCCTCTATTGC ATCCCCCTGCCCGACTCCTTCCCCGCACCCACGTGCCCTAGATTCATGGCAGAAAATGAC CAAATCCTGTGTATTTGTTTTATATATTTAATAACTGTTTTAAATGAAAGTTTTAGTAAA AAAAATACAAAACAAAAAGATTAAATTGCTATTGCTGTAGTAAGAGAAGCTCTTTGTATC TGAACATAGTTGTATTTGAAATTTGTGGTTTTTTAATTTATTTAAAATTGGGGGGAGGGC ATGGGAAGGATTTAACACCGATATATTGTTACCGCTGAAAATGAACTTTATGAACCTTTT CCAAGTTGATCTATCCAGTGACGTGGCCTGGTGGGCGTTTCTTCTTGTACTTATGTGGTT TTTTGGCTTTTAATACAGACATTTTCCTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	NotI-NotI
ACCN	BC057390
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC057390.1, AAH57390.1
RefSeq Size	1798 bp
Locus ID	25907
Protein Families	Druggable Genome
Gene Summary	Constitutive activation of the Ras pathway triggers an irreversible proliferation arrest reminiscent of replicative senescence. Transcription of this gene is upregulated in response to activation of the Ras pathway, but not under other conditions that induce senescence. The encoded protein is similar to a rat cell surface receptor proposed to function in a neuronal survival pathway. An allelic polymorphism in this gene results in both functional and non-functional (frameshifted) alleles; the reference genome represents the functional allele. [provided by RefSeq, Jul 2015]

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