BCO2 (BC041656) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	b,b-carotene-9',10'-dioxygenase; B-DIOX-II; BCDO2; beta,beta-carotene 9',10'-dioxygenase variant 1; beta-carotene 9',10' oxygenase; beta-carotene dioxygenase 2; beta-carotene oxygenase 2; FLJ34464
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC041656 edited GTGCGTGTTCACTGTTTAGTAGTACTCAAAACTGCCAGTGTGAGAGGATTTGGAAATCAC TGGATCTGCTCAATACAAAAATGTTTTTTCGAGTCTTTCTCCATTTTATCAGGAGTCATT CTGCCACTGCAGTGGATTTCCTTCCTGTGATGGTGCACCGGCTCCCAGTTTTCAAAAGGT ACATGGGAAATACTCCTCAGAAAAAAGCCGTCTTTGGGCAGTGTCGGGGTCTGCCATGTG TTGCACCGCTGCTGACCACAGTGGAAGAGGCTCCACGGGGCATCTCTGCTCGAGTCTGGG GACATTTTCCTAAGTGGCTCAATGGCTCTCTACTTCGAATTGGACCTGGGAAATTCGAGT TTGGGAAGGATAAGTACAATCATTGGTTTGATGGGATGGCGCTGCTTCACCAGTTCAGAA TGGCAAAGGGCACAGTGACATACAGGAGCAAGTTTCTACAGAGTGATACATATAAGGCCA ACAGTGCTAAAAACCGAATTGTGATCTCAGAATTTGGCACACTGGCTCTCCCGGATCCAT GCAAGAATGTTTTTGAACGTTTCATGTCCAGGTTTGAGCTGCCTGGTAAAGCTGCAGGTT TCTCCTATAAGGTTATTCGGGTTCCTCCAGAGAAGGTGGACCTTGGGGAGACAATCCATG GAGTCCAGGTGATATGTTCTATTGCTTCTACAGAGAAAGGGAAACCTTCTTACTACCATA GCTTTGGAATGACAAGGAACTATATAATTTTCATTGAACAACCTCTAAAGATGAACCTGT GGAAAATTGCCACTTCTAAAATTCGGGGAAAGGCCTTTTCAGATGGGATAAGCTGGGAAC CCCAGTGTAATACGCGGTTTCATGTGGTGGAAAAACGCACTGGACAGCTCCTTCCAGGGA GATACTACAGCAAACCTTTTGTTACATTTCATCAAATCAATGCCTTTGAGGACCAGGGCT GTGTTATAATTGATTTGTGCTGTCAAGATAATGGAAGAACCCTAGAAGTTTACCAGTTAC AGAATCTCAGGAAGGCTGGGGAAGGGCTTGATCAGGTCCATAATTCAGCAGCCAAATCTT TCCCTCGAAGGTTTGTTTTGCCTTTAAATGTCAGTTTGAATGCCCCTGAGGGAGACAACC TGAGTCCATTGTCCTATACTTCAGCCAGTGCTGTGAAACAGGCTGATGGAACGATCTGGT GCTCTCATGAAAATCTACATCAGGAGGACCTAGAAAAGGAAGGAGGCATTGAATTTCCTC AGATCTACTATGATCGATTCAGTGGCAAAAAGTATCATTTCTTTTATGGCTGTGGCTTTC GGCATTTAGTGGGGGATTCTCTGATCAAGGTTGATGTGGTGAATAAGACACTGAAGGTTT GGAGAGAAGATGGCTTTTATCCCTCAGAACCTGTTTTTGTTCCAGCACCAGGAACCAATG AAGAAGATGGTGGGGTTATTCTTTCTGTGGTGATCACTCCCAACCAGAATGAAAGCAATT TTCTCCTAGTTTTGGATGCCAAGAACTTTGAAGAGCTGGGCCGAGCAGAGGTACCTGTGC AGATGCCTTATGGGTTCCATGGTACCTTCATACCCATCTGATGGGACAACCACAAGGTCT GGAAACTAGGTTTAAAATAAGTGTGCACTTGGACATAAAGACTGGAGAAATAAACACTGA GGACTCCAAAAGGGGGGCAAGGAGGAAGAGGGGCAGGGGTTAAAAAGCTACCTATTGAAT ACTATGTTCCCTATTTGGGTGATGGGTTCGTTAGAAGTCCAAACCTCAGCAGCACACAAT ATACTCATGTAACAAGCCTGCACATGTACCCCAGAATCTAAAATAAAAATAAAAAGAAAA ACAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC041656 unedited TCTGAATTTGTAATACGACTTACTATAGGGNCGGACCGCGNAATTCCCGGGATGTGCGTG TTCACTGTTTAGTAGTACTCAAAACTGCCAGTGTGAGAGGATTTGGAAATCACTGGATCT GCTCAATACAAAAATGTTTTTTCGAGTCTTTCTCCATTTTATCAGGAGTCATTCTGCCAC TGCAGTGGATTTCCTTCCTGTGATGGTGCACCGGCTCCCAGTTTTCAAAAGGTACATGGG AAATACTCCTCAGAAAAAAGCCGTCTTTGGGCAGTGTCGGGGTCTGCCATGTGTTGCACC GCTGCTGACCACAGTGGAAGAGGCTCCACGGGGCATCTCTGCTCGAGTCTGGGGACATTT TCCTAAGTGGCTCAATGGCTCTCTACTTCGAATTGGACCTGGGAAATTCGAGTTTGGGAA GGATAAGTACAATCATTGGTTTGATGGGATGGCGCTGCTTCACCAGTTCAGAATGGCAAA GGGCACAGTGACATACAGGAGCAAGTTTCTACAGAGTGATACATATAAGGCCAACAGTGC TAAAAACCGAATTGTGATCTCAGAATTTGGCACACTGGCTCTCCCGGATCCATGCAAGAA TGTTTTTGAACGTTTCATGTCCAGGTTTGAGCTGCCTGGTAAAGCTGCANGTTTCTCCTA TAAGGTTATTCGGGTTCCTCCAGAGAAAGTGGACCTTGGGGAGACAATCCATGGAGTCCA GGTGATATGTTCTATTGCTTCTACAGAGAAAGGGAAACCTTCTTACTACCATAGCTTTGG AAATGACAGGAAACTATATATNTTTCATTGAACAACCTCTAAAGATGAACCTGTGGNAAA ATTGCCACTTTCTAAATTCNGGGGAAAGGCCCTTTTCAGATGGGGATAAAGCTGGGAACC CCAGTGTAATACGCCGTTTNCATGTGTGGGAAAACGCACTGGACAGCTCCTTCCAGGGA
Restriction Sites	NotI-NotI
ACCN	BC041656
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC041656.1, AAH41656.2
RefSeq Size	1908 bp
Locus ID	83875
Gene Summary	This gene encodes an enzyme which oxidizes carotenoids such as beta-carotene during the biosynthesis of vitamin A. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

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