REEP3 (BC018658) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C10orf74; OTTHUMP00000060744; receptor accessory protein 3; receptor expression enhancing protein 3
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC018658 edited GGGGAGGTAAATGTATCCATAGACCACAGTGCCTTGCTTTTTCCTCTGCCAGCACATGGA GCACGGGATTAGATGCACAAACCTATTTAGGGAACTATTTTGGTAGATGTTTGAGTTTAT ACAGAAATTGCAGCTGGTATTTTATTTTGCTGTACATTTACTCAACTTGTCCATTAGTAT TTAACTATTTCCAGAGTTTGTTTAGGAGTAAGAATTGACCCATTCGTTAGTTTACCATAT TTTTTCCTGGTATAAAAAGGAGCCAGAAATAAGCCTTATTGCTAAATAATTAATTATGTA AGCCCACCTAGGTCCTGCATAAGATCCCCCTCACATACTTCACAATATATATGTGTGTGT GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATTTGGCTAAAAAATTATACTG CCAAAATTACTGATTATAAATACTTGACTACACTGATTGATGGGACAAAATGATTAAAGT ATTTTCAGGGATCTTATTCCATATGTCACCACCAAAGATTTCTACAGTGTTATAAAGTAT ATAAATATTCCAAATTTCTGTGGTTAAATATTTTTTTCTTTTTTTTCCTTTTTTAGAATA ACACAGTCTGTGCTTTCCAAAAATGCTTGAACTTTTATGTTGTTAAAGAAATATATAATG ATATCTTACATTAAGCATGAGTCTAATTTGTATTAATTGGGATGGACTAAATTTTCATTT GATTATCAGGAAAATTAAGGAGTTATATATTTAAAAGCAATTTTCTGTGTTTTCTTCTTT GTAAGTTGACTCATTTGTGAAGCAATTAGGCAAATTTTGAGAAGATCATTGTTATTGTGG TTTGCAGTATATATTTCTTAGTAAATATCACTTAAGATTAAATTTTTCAGAAAGAAAATT ATAGCTTTTTTCCCAAAATATTTTTAAGATTTAATCTTTTTGTAGTATGCTACAGATTTA ATTATATTAACTCTTTTTTAAGACATTGACCATGACTTAACATTTTGCCTTCTAACACCT TTTAAATCTATGTACTTTAATAGTTAAGAGAAAATAAGTTTGCAGATTTTTAATAATCTG TTTGTAAAAGGCTATCTCTAAGCCTAGTATGTGGGTAATTTTACAGGTGTGTTTTTTGAT AACTTTAATATAAAATAAACTCATTTTATTTGTGCCAAAAAAAAAAAAAAAAAAAAAAAA AAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC018658 unedited AGGTTCATAATATTTGTATACGACTCATATAGGCGGCCGCGTATTCGGCCATTACGGCCG GGGAGGTAAATGTATCCATAGACACAGTGCCTTGCTTTTTCCTCTGCCAGCACATGGAGC ACGGGATTAGATGCACAAACCTATTTAGGGAACTATTTTGGTAGATGTTTGAGTTTATAC AGAAATTGCAGCTGGTATTTTATTTTGCTGTACATTTACTCAACTTGTCCATTAGTATTT AACTATTTCCAGAGTTTGTTTAGGAGTAAGAATTGACCCATTCGTTAGTTTACCATATTT TTTCCTGGTATAAAAAGGAGCCAGAAATAAGCCTTATTGCTAAATAATTAATTATGTAAG CCCACCTAGGTCCTGCATAAGATCCCCCTCACATACTTCACAATATATATGTGTGTGTGT GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATTTGGCTAAAAAATTATACTGCC AAAATTACTGATTATAAATACTTGACTACACTGATTGATGGGACAAAATGATTAAAGTAT TTTCAGGGATCTTATTCCATATGTCACCACCAAAGATTTCTACAGTGTTATAAAGTATAT AAATATTCCAAATTTCTGTGGTTAAATATTTTTTTCTTTTTTTTCCTTTTTTAGAATAAC ACAGTCTGGGCTTTCCAAAAAGGCTTGAACTTTTATGTTGTTAAAGAAATATATAATGAT ATCTTAAATTAAGCATGATTCTAATTTGTATTAATGGGGATGGACCAAATTTTCATTTGT ATACCAGGAAAATAAGGGTTATATATTAAAAACAATTTTCTGGGGTTTACTCCTTGAAAG TTGACCCTTTGGGAAACATTTAGCCAATTTTAAAAAATCATGGTATTGGGGTTGCAGAAT ATTTTTTTAGAAAAATACACTAAAG
Restriction Sites	NotI-NotI
ACCN	BC018658
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC018658.1, AAH18658.1
RefSeq Size	1206 bp
Locus ID	221035
Protein Families	Druggable Genome, Transmembrane
Gene Summary	Microtubule-binding protein required to ensure proper cell division and nuclear envelope reassembly by sequestering the endoplasmic reticulum away from chromosomes during mitosis. Probably acts by clearing the endoplasmic reticulum membrane from metaphase chromosomes.[UniProtKB/Swiss-Prot Function]

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