PYCR1 (BC022244) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	P5C; P5CR; P5C reductase; PIG45; PP222; proliferation-inducing protein 45; PYCR; pyrroline-5-carboxylate reductase 1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC022244 edited GCCCTGGCCAAGGGCTTCACAGCAGCAGGCGTCTTGGCTGCCCACAAGATAATGGCTAGC TCCCCAGACATGGACCTGGCCACAGTTTCTGCTCTCAGGAAGATGGGGGTGAAGTTGACA CCCCACAACAAGGAGACGGTGCAGCACAGTGATGTGCTCTTCCTGGCTGTGAAGCCACAC ATCATCCCCTTCATCCTGGATGAAATAGGCGCCGACATTGAGGACAGACACATTGTGGTG TCCTGCGCGGCCGGCGTCACCATCAGCTCCATTGAGAAGAAGCTGTCAGCGTTTCGGCCA GCCCCCAGGGTCATCCGCTGCATGACCAACACTCCAGTCGTGGTGCGGGAGGGGGCCACC GTGTATGCCACAGGCACGCACGCCCAGGTGGAGGACGGGAGGCTCATGGAGCAGCTGCTG AGCAGCGTGGGCTTCTGCACGGAGGTGGAAGAGGACCTGATTGATGCCGTCACGGGGCTC AGTGGCAGCGGCCCCGCCTACGCATTCACAGCCCTGGATGCCCTGGCTGATGGGGGTGTG AAGATGGGACTTCCAAGGCGCCTGGCAGTCCGCCTCGGGGCCCAGGCCCTCCTGGGGGCT GCCAAGATGCTGCTGCACTCAGAACAGCACCCAGGCCAGCTCAAGGACAACGTCAGCTCT CCTGGTGGGGCCACCATCCATGCCTTGCATGTGCTGGAGAGTGGGGGCTTCCGCTCCCTG CTCATCAACGCTGTGGAGGCCTCCTGCATCCGCACACGGGAGCTGCAGTCCATGGCTGAC CAGGAGCAGGTGTCACCAGCCGCCATCAAGAAGACCATCCTGGACAAGGTGAAGCTGGAC TCCCCTGCAGGGACCGCTCTGTCGCCTTCTGGCCACACCAAGCTGCTCCCCCGCAGCCTG GCCCCAGCGGGCAAGGATTGACACGTCCTGCCTGACCACCATCCTGCCACCACCTTCTCT TCTCTTGTCACTAGGGGGACTAGGGGGTCCCCAAAGTGGCCCACTTTCTGTGGCTCTGAT CAGCGCAGGGGCCAGCCAGGGACATAGCCAGGGAGGGGCCACATCACTTCCCACTGGAAA TCTCTGTGGTCTGCAAGTGCTTCCCAGCCCAGAACAGGGGTGGATTCCCCAACCTCAACC TCCTTTCTTCTCTGCTCCCAAACCATGTCAGGACCACCTTCCTCTAGAGCTCGGGAGCCC GGAGGGTCTTCACCCACTCCTACTCCAGTATCAGCTGGCACGGGCTCCTTCCTGAGAGCA AAGGTCAAGGACCCCCTCTGTGAAGGCTCAGCAGAGGTGGGATCCCACGCCCCCTCCCGG CCCCTCCCTGCCCTCCATTCAGGGAGAAACCTCTCCTTCCCGTGTGAGAAGGGCCAGAGG GTCCAGGCATCCCAAGTCCAGCGTGAAGGGCCACAGCCCCTCTTGGCTGCCAAGCACGCA GATCCCATGGACATTTGGGGAAAGGGCTCCTTGGGCTGCTGGTGAACTTCTGTGGCCACC ACCTCCTGCTCCTGACCTCCCTGGGAGGGTGCTATCAGTTCTGTCCTGGCCCTTTCAGTT TTATAAGTTGGTTTCCAGCCCCCAGTGTCCTGACTTCTGTCTGCCACATGAGGAGGGAGG CCCTGCCTGTGTGGGAGGGTGGTTACTGTGGGTGGAATAGTGGAGGCCTTCAACTGATTA GACAAGGCCCGCCCACATCTTGGAGGGCATCTGCCTTACTGATTAAAATGTCAATGTAAT CTAAAAAAAAAAAAAAA
Restriction Sites	Please inquire
ACCN	BC022244
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC022244.1, AAH22244.1
RefSeq Size	1757 bp
Locus ID	5831
Protein Pathways	Arginine and proline metabolism, Metabolic pathways
Gene Summary	This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.