VPS53 (NM_018289) Human Untagged Clone
CAT＃: SC126252
VPS53 (untagged)-Human vacuolar protein sorting 53 homolog (S. cerevisiae) (VPS53), transcript variant 2
CNY 7368.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HCCS1; hVps53L; PCH2E; pp13624 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_018289 edited
GGCGGCTGGAGGCCTGAGTTGGGCTCGCGGCGGGGGTCGGCAGGGGGCCGGGTGGCGGAA TGATGGAGGAGGAGGAACTGGAGTTCGTGGAGGAGCTGGAAGCCGTGCTGCAGCTCACGC CCGAGGTGCAGCTGGCCATCGAGCAGGTGTTTCCAAGCCAGGACCCTCTAGATCGAGCAG ATTTCAATGCTGTTGAGTATATCAATACCCTGTTCCCAACCGAGCAATCTCTGGCGAACA TAGACGAAGTCGTGAACAAAATTAGGCTGAAAATAAGGAGACTGGATGACAATATTCGAA CTGTTGTAAGAGGTCAGACGAACGTGGGGCAGGATGGACGGCAAGTGAAAGAAATCACCC GTGATATTAAGCAATTAGATCACGCCAAACGCCACCTGACCACCTCAATCACCACACTGA ACCACCTGCACATGCTGGCAGGAGGTGTCGACTCCCTCGAAGCCATGACCAGGCGAAGAC AATACGGAGAAGTTGCTAATCTCCTTCAGGGTGTGATGAATGTCCTGGAGCACTTCCACA AGTATATGGGGATTCCGCAGATCCGGCAGCTTTCCGAAAGAGTGAAGGCTGCACAGACTG AGTTAGGACAGCAAATCCTGGCAGATTTTGAAGAAGCGTTTCCTTCCCAGGGCACCAAGA GACCAGGAGGACCCAGCAATGTTCTACGAGATGCATGTCTGGTTGCTAATATTCTAGATC CCAGGATCAAACAGGAAATCATCAAAAAGTTTATTAAACAGCATCTGTCAGAGTATCTGG TACTTTTTCAAGAAAACCAAGATGTTGCCTGGCTGGACAAAATCGACAGACGCTATGCCT GGATAAAACGCCAGCTTGTGGACTATGAGGAGAAATACGGCCGCATGTTTCCACGTGAGT GGTGCATGGCTGAGAGGATTGCGGTGGAATTTTGCCATGTGACAAGGGCAGAACTTGCCA AGATTATGCGTACCAGAGCGAAGGAAATTGAAGTGAAATTGCTTCTTTTTGCTATTCAAA GAACAACTAACTTTGAGGGGTTTCTTGCAAAACGCTTCTCCGGCTGCACCCTGACCGATG GGACCCTGAAAAAGCTTGAGTCTCCACCCCCATCTACCAATCCCTTCCTGGAAGATGAGC CAACACCAGAGATGGAGGAACTGGCAACGGAGAAAGGAGATTTAGATCAACCAAAGAAGC CTAAAGCCCCAGACAATCCATTTCATGGCATTGTTTCCAAGTGTTTTGAGCCTCATCTCT ACGTGTATATCGAATCCCAAGACAAGAACCTCGGAGAGCTGATAGATCGGTTTGTGGCTG ATTTCAAAGCCCAGGGGCCACCTAAGCCCAACACTGATGAAGGGGGTGCCGTGCTCCCCA GCTGCGCCGACCTCTTTGTCTACTACAAGAAGTGCATGGTGCAATGCTCTCAGCTCAGTA CTGGGGAGCCCATGATCGCCCTGACCACCATTTTCCAGAAGTACCTCCGAGAATATGCCT GGAAAATCCTCTCTGGCAACCTGCCCAAAACCACAACCAGCAGTGGAGGACTGACTATCA GCAGCCTCCTCAAGGAAAAGGAGGGCTCAGAAGTAGCCAAGTTCACTCTGGAGGAGCTCT GCCTCATCTGTAACATCCTGAGCACGGCAGAGTACTGTCTGGCCACCACCCAGCAGCTAG AAGAAAAACTCAAAGAAAAAGTGGATGTAAGTCTGATTGAACGAATCAATCTGACTGGAG AGATGGACACGTTCAGCACCGTCATCTCCAGCAGTATTCAGCTGCTGGTTCAGGATCTGG ATGCTGCCTGTGATCCTGCCCTGACTGCCATGAGCAAGATGCAGTGGCAGAACGTGGAGC ACGTTGGTGACCAGAGCCCCTACGTCACCTCTGTCATTCTGCACATCAAGCAGAACGTCC CCATCATCCGTGACAACCTGGCTTCCACACGCAAGTACTTCACTCAGTTCTGCGTTAAAT TTGCAAACTCCTTCATTCCCAAATTCATCACCCACCTCTTCAAGTGCAAGCCAATTAGCA TGGTGGGAGCAGAACAGGTGAGATGGACGTAGTATCAGGCATTTGCCTGGCAGCTTTTGT TGTAGATCAAGCACATATTCTTCTAGTCCAGATCTACTTGGCAGGAATAAAATTGATGAT GTCCCCTGTTTGGGGACAGTATAATGACTCAAAAAAAAAAAAAAAAAAA >OriGene 5' read for NM_018289 unedited
NGGTCGAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGGCGGCTGGA GGCCTGAGTTGGGCTCGCGGCGGGGGTCGGCAGGGGGCCGGGTGGCGGAATGATGGAGGA GGAGGAACTGGAGTTCGTGGAGGAGCTGGAAGCCGTGCTGCAGCTCACGCCCGAGGTGCA GCTGGCCATCGAGCAGGTGTTTCCAAGCCAGGACCCTCTAGATCGAGCAGATTTCAATGC TGTTGAGTATATCAATACCCTGTTCCCAACCGAGCAATCTCTGGCGAACATAGACGAAGT CGTGAACAAAATTAGGCTGAAAATAAGGAGACTGGATGACAATATTCGAACTGTTGTAAG AGGTCAGACGAACGTGGGGCAGGATGGACGGCAAGTGAAAGAAATCACCCGTGATATTAA GCAATTAGATCACGCCAAACGCCACCTGACCACCTCAATCACCACACTGAACCACCTGCA CATGCTGGCAGGAGGTGTCGACTCCCTCGAAGCCATGACCAGGCGAAGACAATACGGAGA AGTTGCTAATCTCCTTCAGGGTGTGATGAATGTCCTGGAGCACTTCCACAAGTATATGGG GATTCCGCAGATCCGGCAGCTTTCCGAAAGAGTGAAGGCTGCACAGACTGAGTTAGGACA GCAAATCCTGGCAGATTTTGAAGAAGCGTTTCCTTCCCAGGGCACCAAGAGACCAGGAGG ACCCAGCAATGGTCTACGAGATGCATGTCTGGTTGCTAATATTCTAGATCCCAGGATCAA ACAGGAAATCATCAAAAAGTTATTAACAGCATCTGTCAGAGTATCTGGTACTTTTTCAAG AAACCCAGATGTGCCTGGCTGGACAAATCGACAGACGCATGCCTGGATAAACGCCAGCTG TGGACTATGAGGAGAATACCGC |
| Restriction Sites | Please inquire |
| ACCN | NM_018289 |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018289.2, NP_060759.2 |
| RefSeq Size | 2209 bp |
| RefSeq ORF | 2013 bp |
| Locus ID | 55275 |
| UniProt ID | Q5VIR6 |
| Gene Summary | This gene encodes a protein with sequence similarity to the yeast Vps53p protein. Vps53p is involved in retrograde vesicle trafficking in late Golgi. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) has multiple differences in the coding region and uses a different segment for its 3' UTR but maintains the reading frame, compared to variant 1. The resulting protein (isoform 2) has a shorter and distinct C-terminus when it is compared to isoform 2. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202970 | VPS53 (Myc-DDK-tagged)-Human vacuolar protein sorting 53 homolog (S. cerevisiae) (VPS53), transcript variant 2 |
CNY 5800.00 |
|
| RC202970L3 | Lenti-ORF clone of VPS53 (Myc-DDK-tagged)-Human vacuolar protein sorting 53 homolog (S. cerevisiae) (VPS53), transcript variant 2 |
CNY 7700.00 |
|
| RC202970L4 | Lenti-ORF clone of VPS53 (mGFP-tagged)-Human vacuolar protein sorting 53 homolog (S. cerevisiae) (VPS53), transcript variant 2 |
CNY 7700.00 |
|
| RG202970 | VPS53 (tGFP-tagged) - Human vacuolar protein sorting 53 homolog (S. cerevisiae) (VPS53), transcript variant 2 |
CNY 6370.00 |
