ECH1 (BC011792) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	delta3,5-delta2,4-dienoyl-CoA isomerase; dienoyl-CoA isomerase; enoyl Coenzyme A hydratase 1, peroxisomal; HPXEL; peroxisomal enoyl-CoA hydratase 1; peroxisomal enoyl-coenzyme A hydratase-like protein
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC011792 edited CGATGGCGGCGGGGATAGTGGCTTCTCGCAGACTCCGCGACCTACTGACCCGGCGACTGA CAGGCTCCAACTACCCGGGACTCAGTATTAGCCTTCGCCTCACTGGCTCCTCTGCACAAG AGGAGGCTTCCGGAGTAGCCCTCGGTGAAGCCCCAGACCACAGCTATGAGTCCCTTCGTG TGACGTCTGCGCAGAAACATGTTCTGCATGTCCAGCTCAACCGGCCCAACAAGAGGAATG CCATGAACAAGGTCTTCTGGAGAGAGATGGTAGAGTGCTTCAACAAGATTTCGAGAGACG CTGACTGTCGGGCGGTGGTGATCTCTGGTGCAGGAAAAATGTTCACTGCAGGTATTGACC TGATGGACATGGCTTCAGACATCCTGCAGCCCAAAGGAGATGATGTGGCCCGGATCAGCT GGTACCTCCGTGACATCATCACTCGATACCAGGAGACCTTCAACGTCATCGAGAGGTGCC CCAAGCCCGTGATTGCTGCCGTCCATGGGGGCTGCATTGGCGGAGGTGTGGACCTTGTCA CCGCCTGTGACATCCGGTACTGTGCCCAGGATGCTTTCTTCCAGGTGAAGGAGGTGGACG TGGGTTTGGCTGCCGATGTAGGAACACTGCAGCGCCTGCCCAAGGTCATCGGGAACCAGA GCCTGGTCAACGAGCTGGCCTTCACCGCCCGCAAGATGATGGCTGACGAGGCCCTGGGCA GTGGGCTGGTCAGCCGGGTGTTCCCAGACAAAGAGGTCATGCTGGATGCTGCCTTAGCGC TGGCGGCCGAGATTTCCAGCAAGAGCCCCGTGGCGGTGCAGAGCACCAAGGTCAACCTGC TGTATTCCCGCGACCATTCGGTGGCCGAGAGCCTCAACTACGTGGCGTCCTGGAACATGA GCATGCTGCAGACCCAAGACCTCGTGAAGTCGGTCCAGGCCACGACTGAGAACAAGGAAC TGAAAACCGTCACCTTCTCCAAGCTCTGAGAGCCCTCGCGTCCCAGGCCCCAGCCAGGGG GCCGGCCTTGTCCCGCCTCATCCACAGAAAGGGAGGATGGGCGATGACAGTTGTTTCTAT GCCTTCTGACCCAGTTTCCCAGTTTATAACTTTATGACAATGAGTTTCTCAAGCCCAAGG CCTTATCTTCACCCCACAAACAATAAAGCAAAGTAAAGAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC011792 unedited GTGCGGTCATTATTTGTATACGACTCACTATAGGCGGCCGCGTAATTCGCACGAGGCGAT GGCGGCGGGGATAGTGGCTTCTCGCAGACTCCGCGACCTACTGACCCGGCGACTGACAGG CTCCAACTACCCGGGACTCAGTATTAGCCTTCGCCTCACTGGCTCCTCTGCACAAGAGGA GGCTTCCGGAGTAGCCCTCGGTGAAGCCCCAGACCACAGCTATGAGTCCCTTCGTGTGAC GTCTGCGCAGAAACATGTTCTGCATGTCCAGCTCAACCGGCCCAACAAGAGGAATGCCAT GAACAAGGTCTTCTGGAGAGAGATGGTAGAGTGCTTCAACAAGATTTCGAGAGACGCTGA CTGTCGGGCGGTGGTGATCTCTGGTGCAGGAAAAATGTTCACTGCAGGTATTGACCTGAT GGACATGGCTTCAGACATCCTGCAGCCCAAAGGAGATGATGTGGCCCGGATCAGCTGGTA CCTCCGTGACATCATCACTCGATACCAGGAGACCTTCAACGTCATCGAGAGGTGCCCCAA GCCCGTGATTGCTGCCGTCCATGGGGGCTGCATTGGCGGAGGTGTGGACCTTGTCACCGC CTGTGACATCCGGTACTGTGCCCAGGATGCTTTCTTCCAGGTGAAGGAGGTGGACGTGGG TTTGGCTGCCGATGTAAGAAACACTGAGCGCCTGCCCAAGGTCATCGGGAACCAGAGCCT GGTCAACGAGCTGGCCCTCACCGCCCGCAAGAAGATGGCTGACGAGCCCCTGCGCAGTGG GCTGGTCAGCCGGGGGTTCACAAACAAGAGGTCATGCTGGAGGCCGCCTTAACGCTGGCG GCCGAAGATTTCCGCAAGGACCCCTGGGGGGTGCAGATCACCAAGTCAAACTGCTTTATN
Restriction Sites	NotI-NotI
ACCN	BC011792
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC011792.2, AAH11792.1
RefSeq Size	1218 bp
Locus ID	1891
Gene Summary	This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. [provided by RefSeq, Jul 2008]

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