RECQ4 (RECQL4) (BC013277) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	RECQ4; RecQ protein-like 4; RecQ protein 4; RTS
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC013277 edited CTCGGGCATGACCAGGAAGCAACGGGAATCTGTCCTGCAGAAGATTCGGGCAGCCCAGGT ACACGTGCTGATGCTGACACCTGAGGCACTGGTGGGGGCGGGAGGCCTCCCTCCAGCCGC ACAGCTGCCTCCAGTTGCTTTTGCCTGCATTGATGAGGCCCACTGCCTCTCCCAGTGGTC CCACAACTTCCGGCCCTGCTACCTGCGCGTCTGCAAGGTGCTTCGGGAGCGCATGGGCGT GCACTGCTTCCTGGGCCTCACAGCCACAGCCACACGCCGCACTGCCAGTGACGTGGCACA GCACCTGGCTGTGGCTGAAGAGCCTGACCTCCACGGGCCAGCCCCAGTTCCCACCAACCT GCACCTTTCCGTGTCCATGGACAGGGACACAGACCAGGCACTGTTGACGCTGCTGCAAGG CAAACGTTTTCAAAACCTCGATTCCATTATCATTTACTGCAACCGGCGCGAGGACACAGA GCGGATCGCTGCGCTCCTCCGAACCTGCCTGCACGCAGCCTGGGTCCCAGGGTCTGGAGG TCGTGCCCCCAAAACCACAGCCGAGGCCTACCACGCGGGCATGTGCAGCCGGGAACGGCG GCGGGTACAGCGAGCCTTCATGCAGGGCCAGTTGCGGGTGGTGGTGGCCACGGTGGCCTT TGGGATGGGGCTGGACCGGCCAGATGTGCGGGCTGTGCTGCATCTGGGGCTGCCCCCAAG CTTCGAGAGCTACGTGCAGGCCGTGGGCCGGGCCGGGCGTGACGGGCAGCCTGCCCACTG CCACCTCTTCCTGCAGCCCCAGGGCGAAGACCTGCGAGAGCTGCGCAGACATGTGCACGC CGACAGCACGGACTTCCTGGCTGTGAAGAGGCTGGTACAGCGCGTGTTCCCAGCCTGCAC CTGCACCTGCACCAGGCCGCCCTCGGAGCAGGAAGGGGCCGTGGGTGGGGAGAGGCCTGT GCCCAAGTACCCCCCTCAAGAGGCTGAGCAGCTTAGCCACCAAGCAGCCCCAGGACCCAG AAGGGTCTGCATGGGCCATGAGCGGGCACTCCCAATACAGCTTACCGTACAGGCTTTGGA CATGCCGGAGGAGGCCATCGAGACTTTGCTGTGCTACCTGGAGCTGCACCCACACCACTG GCTGGAGCTGCTGGCGACCACCTATACCCATTGCCGTCTGAACTGCCCTGGGGGCCCTGC CCAGCTCCAGGCCCTGGCCCACAGGTGTCCCCCTTTGGCTGTGTGCTTGGCCCAGCAGCT GCCTGAGGACCCAGGGCAAGGCAGCAGCTCCGTGGAGTTTGACATGGTCAAGCTGGTGGA CTCCATGGGCTGGGAGCTGGCCTCTGTGCGGCGGGCTCTCTGCCAGCTGCAGTGGGACCA CGAGCCCAGGACAGGTGTGCGGCGTGGGACAGGGGTGCTTGTGGAGTTCAGTGAGCTGGC CTTCCACCTTCGCAGCCCGGGGGACCTGACCGCTGAGGAGAAGGACCAGATATGTGACTT CCTCTATGGCCGTGTGCAGGCCCGGGAGCGCCAGGCCCTGGCCCGTCTGCGCAGAACCTT CCAGGCCTTTCACAGCGTAGCCTTCCCCAGCTGCGGGCCCTGCCTGGAGCAGCAGGATGA GGAGCGCAGCACCAGGCTCAAGGACCTGCTCGGCCGCTACTTTGAGGAAGAGGAAGGGCA GGAGCCGGGAGGCATGGAGGACGCACAGGGCCCCGAGCCAGGGCAGGCCAGACTCCAGGA TTGGGAGGACCAGGTCCGCTGCGACATCCGCCAGTTCCTGTCCCTGAGGCCAGAGGAGAA GTTCTCCAGCAGGGCTGTGGCCCGCATCTTCCACGGCATCGGAAGCCCCTGCTACCCGGC CCAGGTGTACGGGCAGGACCGACGCTTCTGGAGAAAATACCTGCACCTGAGCTTCCATGC CCTGGTGGGCCTGGCCACGGAAGAGCTCCTGCAGGTGGCCCGCTGACTGCACTGCATTGG GGGATGTCGGGTAGAGCTGGGGTTGTCAGAGGCTAGGGCAGTGACTGAGGACCTGGGCAA AACCTGCCACAGGGTGTGGGAACGAGGAGGCTCCAAAATGCAGAATAAAAAATGCTCACT TTGTTTTTATGGGAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC013277 unedited CAAATTTTGTATACGACTCACTATAGGGCGGACCGCGAATTCGCACGAGGCTCGGNGCAT GACCAGGAAGCAACGGGAATCTGTCCTGCAGAAGATTCGGGCAGCCCAGGTACACGTGCT GATGCTGACACCTGAGGCACTGGTGGGGGCGGGAGGCCTCCCTCCAGCCGCACAGCTGCC TCCAGTTGCTTTTGCCTGCATTGATGAGGCCCACTGCCTCTCCCAGTGGTCCCACAACTT CCGGCCCTGCTACCTGCGCGTCTGCAAGGTGCTTCGGGAGCGCATGGGCGTGCACTGCTT CCTGGGCCTCACAGCCACAGCCACACGCCGCACTGCCAGTGACGTGGCACAGCACCTGGC TGTGGCTGAAGAGCCTGACCTCCACGGGCCAGCCCCAGTTCCCACCAACCTGCACCTTTC CGTGTCCATGGACAGGGACACAGACCAGGCACTGTTGACGCTGCTGCAAGGCAAACGTTT TCAAAACCTCGATTCCATTATCATTTACTGCAACCGGCGCGAGGACACAGAGCGGATCGC TGCGCTCCTCCGAACCTGCCTGCACGCAGCCTGGGTCCCAGGGTCTGGAGGTCGTGCCCC CAAAACCACAGCCGANGCCTACCACGNCGGCATGTGCAGCCGGGAACGGCGGCGGNTACA GCGAGCCTTCATGCANGGCCAGTTGCGGGTGGTGGTGCCACGGTGGCCCTTGGATGGGCT GGACCGCCAGAGTGCGGCTGTGCTGATCTGGGCTGCCCCAGCTTCAGACTACTGCAGGCG TGGGCCGNCCNGCGTGACGGCAGCCTGCCACTGCACTCTCCTGAGCCCAGGCGAAGACTG CCAAACTGCCANACTGTGCCGCCGACGCACGACTCCCTGCTGGTAAAGCTGTACANGCGT GTTTCAGCTGGACTGGCCTGGACAAGCCC
Restriction Sites	Please inquire
ACCN	BC013277
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC013277.2, AAH13277.2
RefSeq Size	2137 bp
Locus ID	9401
Protein Families	Druggable Genome, Stem cell - Pluripotency
Gene Summary	The protein encoded by this gene is a DNA helicase that belongs to the RecQ helicase family. DNA helicases unwind double-stranded DNA into single-stranded DNAs and may modulate chromosome segregation. This gene is predominantly expressed in thymus and testis. Mutations in this gene are associated with Rothmund-Thomson, RAPADILINO and Baller-Gerold syndromes. [provided by RefSeq, Jan 2010]

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