TRM1 (TRMT1) (BC018302) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FLJ20244; TRM1; TRM1 tRNA methyltransferase 1; TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae)
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC018302 edited GGGCTAGGCAGCGCGGGGAGGGATTCGGACTCGGGGCGGGCCTGCACGAGGGCATCGGCT CCACAGGGACCAGTCATCCCCAAAACGAGGGGATATCAGCTTACCGAGGCCGCAGGTTTT CCTAGTCCCTACCTCATAGATATGTAGGACATCCCCGGGCCCGGAATGCGGCTCTCTGAC CCTCTCTGTGCCCTCCCCCGCCCCCCGAACCAGGCTTGGCGGGCGGAGGCGCCAGCGGAT GTCTCATGCAAGGATCGTCTCTGTGGCTAAGCCTCACTTTCCGCTCCGCCCGGGTGCTCT CTAGAGCCCGGTTTTTCGAGTGGCAGTCTCCAGGGCTGCCGAATACAGCAGCGATGGAGA ACGGCACCGGGCCCTACGGAGAAGAACGTCCACGTGAAGTCCAGGAGACGACAGTCACCG AGGGGGCTGCCAAAATCGCCTTTCCCAGTGCCAACGAGGTCTTTTATAACCCGGTGCAGG AATTCAATCGGGACCTGACATGTGCTGTGATCACCGAGTTTGCTCGCATTCAGCTTGGGG CCAAAGGAATCCAGATCAAGGTTCCAGGAGAGAAGGACACGCAAAAAGTGGTCGTGGACT TGTCAGAGCAAGAGGAGGAAAAGGTTGAACTGAAAGAGAGTGAAAACCTGGCCTCAGGAG ACCAACCTCGCACAGCGGCCGTGGGGGAGATCTGTGAGGAAGGCCTGCATGTGCTGGAAG GCCTGGCAGCTTCAGGCCTACGTTCCATTCGATTTGCCCTAGAGGTGCCTGGGCTCAGAT CTGTGGTTGCAAACGATGCCTCCACCCGGGCTGTGGATCTCATACGCCGGAATGTCCAGC TCAATGACGTGGCCCACCTGGTACAGCCGAGCCAAGCAGATGCCCGGATGCTGATGTACC AGCACCAGAGGGTGTCGGAGAGGTTTGACGTCATCGATCTGGACCCCTATGGCAGCCCAG CCACCTTCCTGGATGCAGCTGTGCAGGCTGTGAGTGAAGGAGGGTTGCTGTGTGTGACCT GCACAGACATGGCGGTGTTGGCGGGGAACAGCGGGGAGACGTGCTACAGCAAGTACGGGG CCATGGCCCTCAAGAGCCGGGCCTGCCACGAGATGGCCCTGAGAATCGTCCTGCACAGCC TGGACCTCCGCGCCAACTGCTACCAGCGCTTCGTGGTGCCGCTGCTCAGCATCAGCGCTG ACTTCTACGTGCGTGTTTTTGTCCGTGTCTTCACCGGCCAGGCCAAGGTCAAGGCCTCAG CCAGGGCCAAGTTCTCTGCAGCCTGTGGTCCCCCTGTGACCCCCGAGTGTGAACACTGTG GGCAACGACACCAGCTTGGTGGCCCCATGTGGGCAGAGCCCATCCATGACCTGGATTTTG TGGGCCGTGTCCTGGAGGCTGTGAGCGCTAACCCCGGCCGCTTCCACACCTCGGAGCGGA TCCGAGGGGTCCTGAGCGTCATCACTGAGGAGCTCCCGGACGTGCCTCTGTACTACACCC TGGACCAGCTGAGCAGCACCATCCACTGCAACACACCAAGCCTCCTGCAGTTGCGGTCGG CCCTCCTCCACGCTGACTTCCGGGTCTCACTCTCCCACGCCTGTAAGAACGCTGTGAAGA CGGATGCCCCTGCCTCTGCCCTCTGGGACATCATGCGTTGCTGGGAGAAGGAATGTCCGG TGAAACGGGAGCGACTATCAGAGACTAGCCCAGCGTTCCGCATTCTCAGTGTGGAGCCCA GGCTGCAGGCCAACTTCACCATCCGGGAAGATGCCAACCCCAGCTCCCGACAGCGAGGAC TCAAGCGCTTCCAGGCTAACCCGGAGGCCAACTGGGGTCCCCGGCCTCGTGCCCGGCCAG GGGGCAAGGCGGCCGACGAAGCTATGGAGGAGAGACGCAGGCTGCTTCAGAACAAGCGGA AGGAGCCGCCGGAAGATGTGGCCCAGCGGGCTGCCCGGCTCAAGACATTTCCTTGCAAGA GGTTTAAGGAGGGCACCTGTCAACGCGGGGACCAGTGCTGCTACTCCCACAGCCCCCCGA CACCCAGGGTTTCTGCTGATGCTGCCCCTGACTGTCCAGAGACCTCCAACCAGACCCCCC CTGGACCTGGGGCTGCCGCTGGGCCAGGCATAGACTGAACCAATAAAGAGATGTCACGTC ACCTTCAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC018302 unedited CCCCCCCGAATCAGGATTTTGTNAATACGAATTCACTATGAGGGCGGACCGCGNAATTCA GAATCTNNGGTACCGGTCCGGTAATTCCCGGGATATCGTCGACGCCACGCGTCCGGGGCT AGGCAGCGCGGGGAGGGATTCGGACTCGGGGCGGGCCTGCACGAGGGCATCGGCTCCACA GGGACCAGTCATCCCCAAAACGAGGGGATATCAGCTTACCGAGGCCGCAGGTTTTCCTAG TCCCTACCTCATAGATATGTAGGACATCCCCGGGCCCGGAATGCGGCTCTCTGACCCTCT CTGTGCCCTCCCCCGCCCCCCGAACCAGGCTTGGCGGGCGGAGGCGCCAGCGGATGTCTC ATGCAAGGATCGTCTCTGTGGCTAAGCCTCACTTTCCGCTCCGCCCGGGTGCTCTCTAGA GCCCGGTTTTTCGAGTGGCAGTCTCCAGGGCTGCCGAATACAGCAGCGATGGAGAACGGC ACCGGGCCCTACGGAGAAGAACGTCCACGTGAAGTCCAGGAGACGACAGTCACCGAGGGG GCTGCCAAAATCGCCTTTCCCAGTGCCAACGAGGTCTTTTATAACCCGGTGCAGGAATTC AATCGGGACCTGACATGTGCTGTGATCACCGAGTTTGCTCGCATTCAGCTTGGGGCCAAA GGAATCCAGATCAAGGTTCCAGGAGAGAAGGACACGCAAAAAGTGGTCGTGGACTTGTCA GAGCAAGAGGAGGAAAAGTTTGAACTGAAAAAGAGTGAAAACCTGGCCTCAGGAGACCAA CCTCCAAGCGGCCGTGGGGGAAATCTGTGAAGAAAGCCTGCATGTGCCTGGAAGGCCTGG CACTTTCAGCCCACGTTCCATTCGATTTGCCCTAAAGTGCCTGGCCTCACAC
Restriction Sites	Please inquire
ACCN	BC018302
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC018302.1, AAH18302.1
RefSeq Size	2181 bp
Locus ID	55621
Protein Families	Druggable Genome
Gene Summary	This gene encodes a tRNA-modifying enzyme that acts as a dimethyltransferase, modifying a single guanine residue at position 26 of the tRNA. The encoded enzyme has both mono- and dimethylase activity when exogenously expressed, and uses S-adenosyl methionine as a methyl donor. The C-terminal region of the encoded protein has both a zinc finger motif, and an arginine/proline-rich region. Mutations in this gene have been implicated in autosomal recessive intellectual disorder (ARID). Alternative splicing results in multiple transcript variants encoding different isoforms. There is a pseudogene of this gene on the X chromosome. [provided by RefSeq, May 2017]

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