HRASLS5 (BC034222) Human Untagged Clone
CAT＃: SC126033
HRASLS5 (untagged)-Human H-rev107-like protein 5, mRNA (cDNA clone MGC:39540 IMAGE:5268703), complete cds
CNY 2950.00
货期*
4周
规格
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Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | calcium-independent phosphatidylethanolamine N-acyltransferase; H-rev107-like protein 5; HRAS-like suppressor family, member 5; HRLP5; iNAT; lecithin-retinol acyltransferase (LRAT)-like protein-1; RLP-1; RLP1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for BC034222 edited
GCGGTTCCTCCCCGAGCCGCCCCCAGTGCCCAAGCTTCCGCGGCTCCGCCGACCAGGGGC CGCGCCGACTGGGAACTCGCCAGGGGCGTAGTCCCCTGCAAGGCCTGGGGGCCGGCGAGG CCGCAGAGGCGGGATGGGCCTGAGCCCGGGCGCCGAGGGGGAGTACGCGCTCCGCCTCCC TAGGATTCCCCCACCCCTCCCCAAACCCGCCTCGCGAACCGCCGGTACCGGGCCCAAGGA CCAGCCGCCTGCGCTCAGACGTTCAGCTGTGCCCCACTCAGAAGAATCCGTGGGATTCGC AGCGTTGGTCCAGCTCCCAGCCAAGCAGCCTCCGCCGGGCACATTAGAACAGGGCAGAAG CATCCAGCAAGGGGAGAAGGCTGTAGTTAGCTTGGAGACCACACCCAGCCAGAAAGCAGA CTGGAGTTCAATTCCAAAGCCTGAGAATGAAGGCAAGTTAATAAAGCAAGCAGCTGAGGG AAAACCAAGACCCAGACCTGGAGACCTGATTGAGATTTTTCGAATTGGCTATGAGCACTG GGCCATCTATGTAGAAGATGATTGCGTGGTCCATCTGGCTCCCCCAAGTGAGGAGTTTGA GGTGGGCAGCATTACTTCCATCTTTAGCAATCGGGCCGTGGTGAAATACAGTCGTCTGGA GGATGTGCTGCATGGCTGCTCCTGGAAGGTCAATAACAAGCTAGATGGGACGTACCTGCC CTTGCCGGTGGACAAGATCATCCAGCGTACAAAAAAGATGGTCAACAAGATCGTGCAGTA CAGCCTGATTGAAGGGAACTGTGAGCACTTTGTCAATGGCCTCAGATATGGCGTACCCCG GAGCCAGCAGGTAGAGCACGCCCTGATGGAAGGAGCGAAGGCTGCTGGAGCAGTTATTTC AGCTGTAGTGGATAGCATAAAGCCCAAACCAATAACTGCCTGAAGGTGATGAAAACTCCA GCTAGAGGAAGAGTTACTGACACAAGCAAAAAGAACATGCTTCCTTCCCTTGCCTTCTCT CAATGGCTCCGATTAAGAAAATTGTGAACTTCTGGAAGAATCTACAATGTATCCAATTAC CCTTCCAGAAATACATCCAATATATACGAGCATAGACCCACAGACTCTCAGGATGGGAAG GATCTGGTATCTAGTGTGTATCTCTTATGCGCCAGGCACTGTGCTAGGCATTTCATATAC GGATCTAATTTCACTCTCTTTGTAGGATGGGACCTTGCTTGTGTCTTAGGCTGACCTTGG TCTCCGATGCTTGAGTGCCATCAGAAACATCCCAGAAACCTCTGCTTGAATATTTCCAGT AACGAAGATGTCTTTTTCCAGATGTTCTTTTTATTTAACTAAAATGTCTCTAGCTTCTTT CCATTTATGATAATCCTGCCCTTTTCGACCCACAGTGTAGCTCAGAGGAGGAGGACCTTA AGAAAAACTGAGATTCGTTATTCAGGGCCCATGGTACCTTAATCTAGAAACACATATCCC AAGAAATATTTTCTTTTTAAAATTATACATCAACTTTATGCCTTATTGTTTAAAAATCTT CTATCAAAAGTATCATCGATAAGACAATTTATCTTGCTTTTGTACCTCATAATTACCCTA AAATGTGAAGTTTCTAGTCTCACTGATTTCAGATGAATATTTAAAATAATTTGATAAGTT TAATCAATTGAACTCTACTGTGTGAGTGAGGGAGAGAAAGGAGTTATTTTTGTGTTTTGA AGAAAGTTGACAGGATGGAAGATCTGAAATTATGGCTGGGGATTGTAGTGATAGCTGGTA GCTGAAACCACCCAAAAAAAGCTAGAAAAGTGTTCTCTTATGCCTCCAGAGATACCTAGA AAATATGAAGAATGTGGAAAAGAGCAAGATGGAACCCTCAGAGTGTTTCAGATAAGGTCT GTTGGTATCTCCATAAGCTTTTCTCCTATTTGGAGAAAATAGACTCTCACTGAAACTCTT CAATGTTTTTACCCTCCATCCTTTTCTTAGCAATCTAAACTCAATGGCCTCCTGTTCCCC AACACTGCAGTCTGGGCATCTGACTTTGTCTTCCCTCTGCACTCATTTCTGTGAATCATG ACCAGGATGAAACTGATATCCCTAGTTCCTCTTCCCCATTTCTTCTCACACTCCCCTGCC TGAGAATCAGTTGAGTTCCCAATACCTCTTCCTTCAGTCTCTATTCATTATATGGTCTAG GTTGAGACTTCCTTGGAAGCAGAACCTGAGTACCAGGAACTCATATTTTATAAAGTATAT TTATTCCTTTTAGCTGCAAACTAACCCAGCCATGTTTTAGTGAATGCTTTAATCTGAAGA TGACTTTGAGCTCATTGACCATATTAATCTTTCATCAAACAAACTGGATGTTGTTTCATT AACAAAAAACTCCTCCACACCAAGGAGTGTTTAGTAACTTCTGCACAGAGGACTCATCCA TCTCTTGCTTATGTTTGGGACAGAAAGACACTTCTCTGGCTTTTTACTGGCTTGAACCCA ATGGGACAGAGACTCTCTAGAAAGTTTCAGGGCTCACATTCACTTGTTTGTCCAAAAAAT ATTTTTGAGTGTCTCCCAAGTGCCAAACCCTGGCTACTCCCACCTTTGCCTGAGCCCATG AGCGGTAGTCTGGGGGCTGGATGTGAGGAGTTCTGAGTTGGCTAGGAAGACACAAGGCAC AATGGACATGGGGCAAAGTCCACTTGCTGGACATTGGTCCCTTCCACTTCCAAGCTGTCC ATCAGGCTGAGAGATCTGGAATGCATACCCCACACTTTGAAGATGAGGAAGGGCGAGTTT CTCAAAGTGTGTGTGTGTGTTTCTCTTTTAATCTGTAATGGTTTCTGCATTTTGAGGTAG AAACAGAAATCTCTTTTTTGAGTGGGACCACTCGGGTGCGGCTTCTTAGTCTTGTTGATA TAATAAAGCACATGCCCACTCCTCATGGAGTGGAGAGGAGAAAATTCTAAGGGAACGTAC TCTGAATGCAAATGTTTCTGAGATTAAACACGAAAAAAAAAAAAAAAA >OriGene 5' read for BC034222 unedited
TCTGAATTTGTAATACGAACTTACTATAGGGCGGCCGCATAACTTCGTATAGCATACATT ATACGAAGTTATGGATCAGGCCAAATCGGCCGAGCTCGAATTCGTCGAGAGCGGGCGGTT CCTCCCCGAGCCGCCCCCAGTGCCCAAGCTTCCGCGGCTCCGCCGACCAGGGGCCGCGCC GACTGGGAACTCGCCAGGGGCGTAGTCCCCTGCAAGGCCTGGGGGCCGGCGAGGCCGCAG AGGCGGGATGGGCCTGAGCCCGGGCGCCGAGGGGGAGTACGCGCTCCGCCTCCCTAGGAT TCCCCCACCCCTCCCCAAACCCGCCTCGCGAACCGCCGGTACCGGGCCCAAGGACCAGCC GCCTGCGCTCAGACGTTCAGCTGTGCCCCACTCAGAAGAATCCGTGGGATTCGCAGCGTT GGTCCAGCTCCCAGCCAAGCAGCCTCCGCCGGGCACATTAGAACAGGGCAGAAGCATCCA GCAAGGGGAGAAGGCTGTAGTTAGCTTGGAGACCACACCCAGCCAGAAAGCAGACTGGAG TTCAATTCCAAAGCCTGAGAATGAAGGCAAGTTAATAAAGCAAGCAGCTGAGGGAAAACC AAGACCCAGACCTGGAGACCTGATTGAGATTTTTCGAATTGGCTATGAGCACTGGGCCAT CTATGTAGAAGATGATTGCGTGGTCCATCTGGCTCCCCAAGTGAGGAGTTTGAGGTGGGC AGCATTACTTCCATCTTTAGCAATCGGGCCGTGGTGAAATACAGTCGTCTGGGAGATGTG CTGCATGGCTGCTCCTGGAAGGTNNCATACAGCTAGATGGGACGTACCTGCCCTTGCCGG TGGGACAGATCATCCAGCGTACANNAAGATGGTAACAAGATCGTGCAGTACAGCCTGATT GAAAGGAACTGTGAGCACTTTGTCATGGCCTCAGAATGGCGTACCCA |
| Restriction Sites | NotI-NotI |
| ACCN | BC034222 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC034222.1, AAH34222.1 |
| RefSeq Size | 3048 bp |
| Locus ID | 117245 |
| Gene Summary | Exhibits both phospholipase A1/2 and acyltransferase activities (PubMed:22825852, PubMed:26503625). Shows phospholipase A1 (PLA1) and A2 (PLA2) activity, catalyzing the calcium-independent release of fatty acids from the sn-1 or sn-2 position of glycerophospholipids (PubMed:22825852). Shows N-acyltransferase activity, catalyzing the calcium-independent transfer of a fatty acyl group at the sn-1 position of phosphatidylcholine (PC) and other glycerophospholipids to the primary amine of phosphatidylethanolamine (PE), forming N-acylphosphatidylethanolamine (NAPE), which serves as precursor for N-acylethanolamines (NAEs) (PubMed:19000777, PubMed:22825852).[UniProtKB/Swiss-Prot Function] |
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