LOC554223 (BC051362) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC051362 edited GAAACATTGAGACAGAGCGCTTGGCACAGAAGTAGCGGGGTCAGGGCGAAGTCCCAGGGC CTCAGGCGTGGCTCTCAGGATCTCAGGCCCCAAAGGCGGTGTATGGATTGGGGAGGCCCA GCGCTGGGCATTCCCCATCTTTGCAGGGTTTCTCTTCTCCCTCTCCCAACCTGTGTCGGG TCCTTCTTCCTGGGTACTCACCGGGCTGCCCCAGTTCTCACTCCCATTGAGTGTCGGGTT TCTAGAGAAGCCAATCAATGTAGCCGCGGTCCCGGTTCTAAAGTTCCCACGCACCCACCG GGACTCCGATTCTTCCCAGTCGCCGAGGATGGTGTCATGGCGCCCCGAACCCTGCTTCTG CTGCTCTCGGGGGCCCTGGTCCTGACCCAGACCTGGGCAGGCTTCCACTCCTTGAGGTAT TTCCACACCACCATGTCCCGGCCCGGCCGCGCGGATCCCCGCTTCCTCTCCGTGGGCGAC GTGGACGACACGCAGTGCGTGCGGCTCGACAGCGACGCCACGAGTCCCAGGATGGAGCCG CGGGCGCCGTGGATGGAGCAGGAGGGGCCGGAATATTGGGAAGAGGAGACAGGGACCGCC AAGGCCAAAGCACAGTTTTACCGAGTGAACCTGCGGACCCTGAGCGGCTACTACAACCAG AGTGAGGCCTAAGTGCAGCTTCATTCCCTCCCTGTTCGTGTGGCCTGGACTTAATGACTC ACTTCTAACTGATAGAGTAATGCTGACATAATAGTTTGTGATTCTGGGTGTAGAACATAA GACTCACTGAAGTTTCTACTTTGGTTCTTTCTTTCTCTGGAATCATGAGCCCTGGGGGAA GCTGGCTGTTGTGTCATAAGGAGGCCTGTGGTCCATGTGACTAGGAAGTGAGTCCTCCTG GGACCAGACAATAAGAAGCTAAAGCCTCTTCCAAAAGCCATGTGAGAGATTCTTGTGTCT TGTGAATCCCCGGCCCCATTTGAGCCCTCAGATGATTCAGCCCTGGAAGACAACTAGACT GCAACGTTGTGAGAGGCCCTGAGCCAGAAGCATTCAGAGAAACTTCTCCTGGATTCCTGA CCATGGATAACTGTGGGAGATGATAAATATTTGTTGATTTGAGCTGCTAAGTTGTAGGTG ACTTGTTATGCAGCAGTAGATAACTAATACAGCTTCACAAGAGAGGATGAATCACTGAAC TTTTTCATTTGCTCTAAATTCATTATAAGATATTAAACATGTCATTTGCTTTTAATATTT AATAAAAATTTCCATGGCTATATAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC051362 unedited GCCTGAATTTGTATCCACTTATATAGGCGGCCGCGTAATTCGCACGAGGAAACATTGANA CAGACGCTTGGCACAGAAGTAGCGGGGTCAGGGCGAAGTCCCAGGGCCTCAGGCGTGGCT CTCAGGATCTCAGGCCCCAAAGGCGGTGTATGGATTGGGGAGGCCCAGCGCTGGGCATTC CCCATCTTTGCAGGGTTTCTCTTCTCCCTCTCCCAACCTGTGTCGGGTCCTTCTTCCTGG GTACTCACCGGGCTGCCCCAGTTCTCACTCCCATTGAGTGTCGGGTTTCTAGAGAAGCCA ATCAATGTAGCCGCGGTCCCGGTTCTAAAGTTCCCACGCACCCACCGGGACTCCGATTCT TCCCAGTCGCCGAGGATGGTGTCATGGCGCCCCGAACCCTGCTTCTGCTGCTCTCGGGGG CCCTGGTCCTGACCCAGACCTGGGCAGGCTTCCACTCCTTGAGGTATTTCCACACCACCA TGTCCCGGCCCGGCCGCGCGGATCCCCGCTTCCTCTCCGTGGGCGACGTGGACGACACGC AGTGCGTGCGGCTCGACAGCGACGCCACGAGTCCCAGGATGGAGCCGCGGGCGCCGTGGA TGGAGCAGGAGGGGCCGGAATATTGGGAAGAGGAGACAGGGACCGCCAAGGCCAAAGCAC AGTTTTACCGAGTGAACCTGCGGACCCTGAGCGGCTACTACAACCAGAGTGAGGCCTAAG TGCAGCTTCATTCCCTCCCTGTTCGTGTGGCCTGGACTAATGACTCACTTCTAACTGATA GAGTAATGCTGACATAATAGTTTGTGATTCTGGGTGTAGAACATAAGACTCACTGAAGTT TCTACTTTGGTTCTTTCTTTCTCTGGGATCATGAACCCCTGGGGAAAGCTGGCTGGTGTG TCATAAAGAGGCCTGGTGGTCATGTGACCTAGGAAATGAGTCCTCCCGGT
Restriction Sites	NotI-NotI
ACCN	BC051362
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC051362.1, AAH51362.1
RefSeq Size	1301 bp
Locus ID	554223

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