THG1L (BC001852) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FLJ11601; FLJ20546; ICF45; interphase cytoplasmic foci protein 45; tRNA-histidine guanylyltransferase 1-like (S. cerevisiae)
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC001852 edited GTGGGGCGCCTGTAAAGTTAAGGTTCACGATTCCTTGGCCACCATTTCCATCACTCTGAG ACGGTACCTGAGATTGGGGGCGACCATGGCAAAAAGCAAGTTCGAGTACGTGAGGGACTT CGAGGCTGACGACACCTGCCTGGCACACTGCTGGGTGGTAGTGCGGCTGGACGGCCGGAA TTTCCATCGGTTTGCTGAGAAGCACAACTTTGCAAAACCCAATGACAGCCGTGCTCTCCA GCTGATGACCAAATGTGCGCAGACTGTGATGGAAGAACTAGAGGATATTGTGATCGCGTA TGGACAGAGTGATGAGTACAGCTTTGTGTTCAAGCGGAAAACCAATTGGTTTAAAAGAAG AGCCAGTAAGTTCATGACTCACGTGGCCTCCCAGTTTGCCTCCAGCTATGTGTTTTATTG GCGGGATTACTTTGAGGACCAGCCCCTTCTGTATCCCCCAGGCTTTGACGGAAGAGTCGT GGTGTATCCCAGCAACCAGACTTTAAAGGACTACCTCAGCTGGCGACAAGCAGATTGTCA CATCAATAATCTTTATAATACAGTTTTCTGGGCACTTATACAACAATCTGGACTAACACC AGTACAAGCCCAAGGGAGATTACAGGGAACTCTTGCAGCAGACAAGAATGAGATTTTGTT TTCTGAATTCAACATCAACTATAATAATGAGCTGCCGATGTATAGGAAAGGGACTGTGTT GATATGGCAGAAGGTGGATGAAGTGATGACAAAAGAAATTAAGCTGCCAACAGAAATGGA AGGAAAAAAGATGGCAGTGACCCGGACCAGGACAAAGCCAGTGCCCTTGCACTGCGATAT CATCGGGGATGCTTTCTGGAAGGAACATCCAGAGATTCTAGATGAAGACAGCTGACCCTT TTGCGCTTCAGTTCTGGTGTGCTTAACCATGCAAGCCCTCCCACCTCCCAGGGCTCCTTG CCTTAGGTGGCTGTAGCATCCCTACCACCCAGGACACTGGTGCGAATGACACAACTCAAG TTGGGAGGGGAACAGGGAAGGAAGGGATGGATGGGGGTGGTGTATCTTACTCTGTTTAAG CAGAACACCTTGTTTGCGGTGTTGGAACATGGTTCCTTTGGCAGAAGTGCTTTTTTTTTA ATCGCAGTACTATTTTTATAAAGCAAGAACTATTCCAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	Please inquire
ACCN	BC001852
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC001852.2, AAH01852.2
RefSeq Size	1199 bp
Locus ID	54974
Gene Summary	The protein encoded by this gene is a mitochondrial protein that is induced by high levels of glucose and is associated with diabetic nephropathy. The encoded protein appears to increase mitochondrial biogenesis, which could lead to renal fibrosis. Another function of this protein is that of a guanyltransferase, adding GMP to the 5' end of tRNA(His). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2015]

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