Kv1.2 (KCNA2) (BC043564) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HBK5; HK4; HUKIV; KV1.2; MGC50217; MK2; NGK1; potassium channel; potassium voltage-gated channel, shaker-related subfamily, member 2; RBK2; voltage-gated potassium channel protein Kv1.2
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC043564 edited GGCACATAGAAGAGTGAGCCATCATCTGGTTTCCAGCGCCAAGACTTCTGCAGGAGAGCC TCCGCTGGGCTTTCAAGTTGGCAACGTCACACCTCCTGAGGACAGCCAGGACTCCAGCTT TTGCTGAGCTTTGCATCTTGCCTCCTTCCTTCAAAAGGCTCCAGGCCCATGCTCGGCTGT CTTCAAGTCCCGCATGCCCCTGGCCAGGCACCTCACCGCCCCTCCAGCCCAGCCCAATCC TAGAGCTCACCTCCAGGACCCAGAGTCTGCCCTTCTGCCCAGAATGACTCACCATTATTT CTAGCTCGAGTGAGAAGACGTGATGAGGAGTTGGGCTGCCTGTTGGTGCATCTCAGATTC CTGCAGCATCTGATCAGCTCTGCAAGGAAGAGAGCTTCACGTCTCTGGTCAGCCAAGCGA GGCTGTCTCTCCAGCTCTCAGAGAGCTCTCGGGGCTCTCCTGCAGGAGACCAGGCCAATG CTCCTGTGCTTCCTGGGGCCAGTAGCAGCACCCTGAGCTCCCTGCCACCAGGCAGCTGAA AGGCATAGCGTGAGGTGCTTCTCTCAGTCCCAATTATGACAGTGGCCACCGGAGACCCAG CAGACGAGGCTGCTGCCCTCCCTGGGCACCCACAGGACACCTATGACCCAGAGGCAGACC ACGAGTGCTGTGAGAGGGTGGTGATCAACATCTCAGGGCTGCGGTTTGAGACCCAGCTAA AGACCTTAGCCCAGTTTCCAGAGACCCTCTTAGGGGACCCAAAGAAACGAATGAGGTACT TTGACCCCCTCCGGAATGAGTACTTTTTCGATCGGAACCGCCCTAGCTTTGATGCCATTT TGTACTACTACCAGTCAGGGGGCCGATTGAGGCGACCTGTGAATGTGCCCTTAGATATAT TCTCTGAAGAAATTCGGTTTTATGAGCTGGGAGAAGAAGCGATGGAGATGTTTCGGGAAG ATGAAGGCTACATCAAGGAGGAAGAGCGTCCTCTGCCTGAAAATGAGTTTCAGAGACAAG TGTGGCTTCTCTTTGAATACCCAGAGAGCTCAGGGCCTGCCAGGATTATAGCTATTGTGT CTGTCATGGTGATTCTGATCTCAATTGTCAGCTTCTGTCTGGAAACATTGCCCATCTTCC GGGATGAGAATGAAGACATGCATGGTAGTGGGGTGACCTTCCACACCTATTCCAACAGCA CCATCGGGTACCAGCAGTCCACTTCCTTCACAGACCCTTTCTTCATTGTAGAGACACTCT GCATCATCTGGTTCTCCTTTGAATTCTTGGTGAGGTTCTTTGCCTGTCCCAGCAAAGCCG GCTTCTTCACCAACATCATGAACATCATTGACATTGTGGCCATCATCCCCTACTTCATCA CCCTGGGGACAGAGTTGGCTGAGAAGCCAGAGGACGCTCAGCAAGGCCAGCAGGCCATGT CACTGGCCATCCTCCGTGTCATCCGGTTGGAACGCAGACCTCTGCAAAGCCAGAAGAGTA AGCGGGGAAGGCAGCATCTGAACACCTCACATGACTGCACCTTAGGAATTAACCTAGTCG CGGGCATGACTGTACAGTGGACCAGGGCATCTGGTCCTGATGACAGGCAGACACCAGCTG TAACTACATTGCACAGGATGTATTGATATTATCATTCTTGCTGACATATTCTGGAAGCTT TCAGCCCCACCAACACTGCCCCACCCAGGACTTCTGCAGCTGAGAGTTCCTGTGTCAGTA GGGTCTGATATTTGTACATAGGTTATACATATATGTGTACATATGTGCCTCAATGAACAT TGCCTGTCCACCTGATCATGGGTGTACATAGACTTCATAGAGCTCCACAGTCTTTTGTAA ATATTGACACAAGTAAATAAGTATATAAATATATATTACTGGGGATTTATTTTAACATCA TTCTGTGTGATATGAAAAAAATAAAATTTTTGACAGACAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC043564 unedited NGGCGTTCAAATTTGTATACGACTCACTATGGGCGGCCGCGAATCAAATCTGGTACCGGT CCGGAATTCCCGGGATGGCACATAGAAGAGTGAGCCATCATCTGGTTTCCAGCGCCAAGA CTTCTGCAGGAGAGCCTCCGCTGGGCTTTCAAGTTGGCAACGTCACACCTCCTGAGGACA GCCAGGACTCCAGCTTTTGCTGAGCTTTGCATCTTGCCTCCTTCCTTCAAAAGGCTCCAG GCCCATGCTCGGCTGTCTTCAAGTCCCGCATGCCCCTGGCCAGGCACCTCACCGCCCCTC CAGCCCAGCCCAATCCTAGAGCTCACCTCCAGGACCCAGAGTCTGCCCTTCTGCCCAGAA TGACTCACCATTATTTCTAGCTCGAGTGAGAAGACGTGATGAGGAGTTGGGCTGCCTGTT GGTGCATCTCAGATTCCTGCAGCATCTGATCAGCTCTGCAAGGAAGAGAGCTTCACGTCT CTGGTCAGCCAAGCGAGGCTGTCTCTCCAGCTCTCAGAGAGCTCTCGGGGCTCTCCTGCA GGAGACCAGGCCAATGCTCCTGTGCTTCCTGGGGCCAGTAGCAGCACCCTGAGCTCCCTG CCACCAGGCAGCTGAAAGGCATAGCGTGAGGTGCTTCTCTCAGTCCCAATTATGACAGTG GCCACCGGAGACCCAGCAGACGAGGCTGCTGCCCTCCCTGGGCACCCACAGGACACCTAT GACCCAGAGGCAGACCACGAGTGCTGTGAGAGGGTGGTGATCAACATCTCAGGGCTGCNG TTTGAGACCCAGCTAAAGACTTAGNCCCATTTCCAGAGACCCTCTTTAGGGACCCAAGAA ACGAATGGAGTACTTTGACCCCCTCCGAATGA
Restriction Sites	NotI-NotI
ACCN	BC043564
Insert Size	2000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC043564.1, AAH43564.1
RefSeq Size	2022 bp
RefSeq ORF	1068 bp
Locus ID	3737
Protein Families	Druggable Genome, Ion Channels: Potassium, Transmembrane
Gene Summary	Potassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shaker-related subfamily. This member contains six membrane-spanning domains with a shaker-type repeat in the fourth segment. It belongs to the delayed rectifier class, members of which allow nerve cells to efficiently repolarize following an action potential. The coding region of this gene is intronless, and the gene is clustered with genes KCNA3 and KCNA10 on chromosome 1. [provided by RefSeq, Jul 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.