ZFYVE19 (BC015738) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ANCHR; MPFYVE
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC015738 edited TCTAGGAGACAGGGGCCACGGGGAGAGCACAGCCACTCGGCGCGAAGAGCCCTCTGTACC CCGCTTCCTCTTGAGGCCCTCGGACCGTCCAGGAAATGTCTGGGAGCCCGCCTGCGGAGG GCATAGCGCCGACCCTCGCTCCCCGCCCAGGACCCGAATGAACCTGGAGAGCGGATGCCA GCAGTGGCCGAGGCGCTAGGACAGGAAGGACCGCCAGACCTCTCAAGATCAGCCTTCCTC GCCACCGTTCTCACCTCTTTGTCCGCTGCCTTCTCCTCAATGCCTAGCAGCGCGTACAGG TCCATCTGTAAGAGCTCCTTGGTCACTGCCATGGTTCCGGCCTGACGGATTCGTACTACA ACTCCCAAGAGTCTAAGCGCGCGTGAGGACTGCAGGCTCCGAGCGGCGCCTAGCCCTCTG GGAATTGTGTTCTGGGTCAGGCTTGACTGACTCTGAGGGAGGCCGGCAGTCGTGAATGAA CTACGACTCCCAGCAGCCCCCGTTGCCGCCGCTGCCGTACGCTGGCTGCAGGAGAGCGTC CGGATTCCCTGCTCTAGGTCGCGGCGGGACAGTGCCAGTGGGCGTGTGGGGCGGGGCGGG GCAGGGCAGGGAAGGGAAGGGCGGAGCTGGGGTGAGGGTCCAAGGGGCCCAGGACTTGGC CGGCGTGATCTCAGCTCTGCAGACCCTGCGGTGCTGGGAGCCACCATGGAGAGTAGGTGC TACGGCTGCGCTGTCAAGTTCACCCTCTTCAAGAAGGAGTACGGCTGTAAGAATTGTGGC AGGGCCTTCTGTTCAGGCTGCCTAAGCTTCAGTGCAGCAGTGCCTCGGACTGGGAACACC CAACAGAAAGTCTGCAAGCAATGCCATGAGGTCCTGACCAGAGGGTCTTCTGCCAATGCC TCCAAGTGGTCACCACCTCAGAACTATAAGAAGCGTGTGGCAGCCTTGGAAGCCAAGCAA AAGCCCAGCACTTCCCAGAGCCAGGGACTGACACGACAAGACCAGATGATTGCTGAGCGC CTAGCACGACTCCGCCAGGAGAACAAGCCCAAGTTAGTCCCCTCACAGGCAGAGATAGAG GCACGGCTGGCTGCCCTAAAGGATGAACGTCAGGGTTCCATCCCTTCCACCCAGGAAATG GAGGCACGACTTGCAGCGTTGCAGGGCAGAGTTCTACCTTCTCAAACCCCCCAGCCGGCA CATCACACACCGGACACCAGGACCCAAGCCCAGCAGACACAGGATCTGCTAACGCAGCTG GCAGCTGAGGTGGCTATCGATGAAAGCTGGAAAGGAGGAGGCCCAGCTGCCTCTCTCCAG AATGATCTCAACCAGGGTGGCCCAGGGAGCACTAATTCCAAGAGGCAGGCCAACTGGTCC TTGGAGGAGGAGAAGAGCAGACTGCTGGCTGAGGCAGCACTTGAGTTGCGGGAGGAGAAC ACGAGGCAGGAACGGATTCTGGCCCTGGCCAAGCGACTAGCCATGCTGCGGGGACAGGAC CCCGAGAGAGTGACCCTCCAGGACTATCGCCTCCCAGACAGTGATGACGACGAGGATGAG GAGACAGCCATCCAAAGAGTCCTGCAGCAGCTCACTGAAGAAGCTGCCCTGGATGAGGCA AGTGGCTTTAACATCCCTGCAGAGCAGGCTTCTCGACCCTGGACGCAACCCCGCGGGGCA GAGCCTGAGGCCCAGGATGTGGACCCCAGGCCTGAGGCTGAGGAAGAGGAGCTCCCCTGG TGCTGCATCTGCAATGAGGATGCCACCCTACGCTGCGCTGGCTGCGATGGGGACCTCTTC TGTGCCCGCTGCTTCCGAGAGGGCCATGATGCCTTTGAGCTTAAAGAGCACCAGACATCT GCCTACTCTCCTCCACGTGCAGGCCAAGAGCACTGAAGACACCCTGGTCCTCCCGGAAGG GCAGTCCCACAGGCAGCGGCACCCATTTCTGGGCCCAGCCACAGGACGTCCGATGGGAGA GCTTGTCTGGCTCTACTGATGATGGATAGGCCCCTTCCTGAGCCTTGGTGTCCCTGGAAT GAGGAAAGATTCTCCATTCGAGAGAATGACTGGGAGGGAAGAAGTCGGGGCCCTCCTATT AGAAGCCCAGACTGGAAGTGAGAGGCATGATGGGGAGAGACCAGACTGAATCTACGGGTG AGCCCTGTAACCTGGCTCTAGGGCACAGGCCCCTCCCCTGGCACTTAGTGGGTCTAATAA AGTATGTTGATTCATTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC015738 unedited NAGTTCGAATTTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGGTCTAGG NAAACAGGNGGCCACGGGGNAGAGCACAGCCACTCGGCGCGAAGAGCCCTCTGTACCCCG CTTCCTCTTGAGGCCCTCGGACCGTCCAGGAAATGTCTGGGAGCCCGCCTGCGGAGGGCA TAGCGCCGACCCTCGCTCCCCGCCCAGGACCCGAATGAACCTGGAGAGCGGATGCCAGCA GTGGCCGAGGCGCTAGGACAGGAAGGACCGCCAGACCTCTCAAGATCAGCCTTCCTCGCC ACCGTTCTCACCTCTTTGTCCGCTGCCTTCTCCTCAATGCCTAGCAGCGCGTACAGGTCC ATCTGTAAGAGCTCCTTGGTCACTGCCATGGTTCCGGCCTGACGGATTCGTACTACAACT CCCAAGAGTCTAAGCGCGCGTGAGGACTGCAGGCTCCGAGCGGCGCCTAGCCCTCTGGGA ATTGTGTTCTGGGTCAGGCTTGACTGACTCTGAGGGAGGCCGGCAGTCGTGAATGAACTA CGACTCCCAGCAGCCCCCGTTGCCGCCGCTGCCGTACGCTGGCTGCAGGAGAGCGTCCGG ATTCCCTGCTCTAGGTCGCGGCGGGACAGTGCCAGTGGGCGTGTGGGGCGGGGCGGGGCA GGGCAGGGAAGGGAAGGGCGGAGCTGGGGTGAGGGTCCAAGGGGCCCAGGACTTGGCCGG CGTGATCTCAGCTCTGCAGACCCTGCGGTGCTGGGAGCCACCATGGAGAGTANGTGCTAC NGCTGCGCTGTCAAGTTCACCCTCTTTCAGAAGAGTACGGCTGTNAGAATGTGGCANGGC CTTCTGTNANGCTGCCTAAGCTCAGTGCAGCAGTGCCTCGNACTGGGAACACCCACAGAA AGTCTGCAGCCATGCCATGAGGT
Restriction Sites	Please inquire
ACCN	BC015738
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC015738.1
RefSeq Size	2290 bp
Locus ID	84936
Gene Summary	Key regulator of abscission step in cytokinesis: part of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage. Together with CHMP4C, required to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis. Deactivation of AURKB results in dephosphorylation of CHMP4C followed by its dissociation from ZFYVE19/ANCHR and VPS4 and subsequent abscission.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.