PFKL (NM_001002021) Human Untagged Clone
CAT＃: SC125729
PFKL (untagged)-Human phosphofructokinase, liver (PFKL), transcript variant 1
CNY 6064.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ATP-PFK; PFK-B; PFK-L |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_001002021 edited
CGTGTTTCGGCCGCCGCCATGGCCGCGGTGGACCTGGAGAAGCTGCGGGCGTCGGGCGCG GGCAAGGCCATCGGCGTCCTGACCAGCGGCGGCGACGCGCAAGGTCCCCTGACAAGCCCA CCAGGCCCCCTGCTGAGATGGCTGTGACCCTGGGCTGACCCGCCCAGTGGCACATTGACT CCGCCTGGAGCTGGGGAGACCAGAGAGGCCCTGTGGTTGGACGGTGGCCTGGGTGCGCTG CTCCTGCCCTCTCCTTGCCCTGCCTCAGCTGCTGCCTGCCAGAGGCGTGGCACCTCACCT CACACCTGCTCCCTGCTGCTGAGCCCCACGCCAAGCTGGAGAGCGGATGAGAAGCATGTG TAACCAGGGTAGAGGTCGAGAGTCCTCTCGTGGGGGTCTCCATGTTCAAGGGAGCTGCCG AGGCTTGAGCAGGAGCCCCCAGCAGGAAACTGGCTTTGCCAAGGCCCCCGCTGGGACAGA CTGTTTCTTTCACTGCAGTCCTGGGAGCCGAGGGCAAGGGGACAGGAAAGAGGAAGTGAC CTCAGAGCCTGGTGGCACCAGCATCATGTCCAGGCTGGGGGGCATGAACGCTGCTGTCCG GGCTGTGACGCGCATGGGCATTTATGTGGGTGCCAAAGTCTTCCTCATCTACGAGGGCTA TGAGGGCCTCGTGGAGGGAGGTGAGAACATCAAGCAGGCCAACTGGCTGAGCGTCTCCAA CATCATCCAGCTGGGCGGCACTATCATTGGCAGCGCTCGCTGCAAGGCCTTTACCACCAG GGAGGGGCGCCGGGCAGCGGCCTACAACCTGGTCCAGCACGGCATCACCAACCTGTGCGT CATCGGCGGGGATGGCAGCCTTACAGGTGCCAACATCTTCCGCAGCGAGTGGGGCAGCCT GCTGGAGGAGCTGGTGGCGGAAGGTAAGATCTCAGAGACTACAGCCCGGACCTACTCGCA CCTGAACATCGCGGGCCTAGTGGGCTCCATCGATAACGACTTCTGCGGCACCGACATGAC CATCGGCACGGACTCGGCCCTCCACCGCATCATGGAGGTCATCGATGCCATCACCACCAC TGCCCAGAGCCACCAGAGGACCTTCGTGCTGGAAGTGATGGGCCGGCACTGCGGGTACCT GGCGCTGGTATCTGCACTGGCCTCAGGGGCCGACTGGCTGTTCATCCCCGAGGCTCCACC CGAGGACGGCTGGGAGAACTTCATGTGTGAGAGGCTGGGTGAGACTCGGAGCCGTGGGTC CCGACTGAACATCATCATCATCGCTGAGGGTGCCATTGACCGCAACGGGAAGCCCATCTC GTCCAGCTACGTGAAGGACCTGGTGGTTCAGAGGCTGGGCTTCGACACCCGTGTAACTGT GCTGGGCCACGTGCAGCGGGGAGGGACGCCCTCTGCCTTCGACCGGATCCTGAGCAGCAA GATGGGCATGGAGGCGGTGATGGCGCTGCTGGAAGCCACGCCTGACACGCCGGCCTGCGT GGTCACCCTCTCGGGGAACCAGTCAGTGCGGCTGCCCCTCATGGAGTGCGTGCAGATGAC CAAGGAAGTGCAGAAAGCCATGGATGACAAGAGGTTTGACGAGGCCACCCAGCTCCGTGG TGGGAGCTTCGAGAACAACTGGAACATTTACAAGCTCCTCGCCCACCAGAAGCCCCCCAA GGAGAAGTCTAACTTCTCCCTGGCCATCCTGAATGTGGGGGCCCCGGCGGCTGGCATGAA TGCGGCCGTGCGCTCGGCGGTGCGGACCGGCATCTCCCATGGACACACAGTATACGTGGT GCACGATGGCTTCGAAGGCCTAGCCAAGGGTCAGGTGCAAGAAGTAGGCTGGCACGACGT GGCCGGCTGGTTGGGGCGTGGTGGCTCCATGCTGGGGACCAAGAGGACCCTGCCCAAGGG CCAGCTGGAGTCCATTGTGGAGAACATCCGCATCTATGGTATTCACGCCCTGCTGGTGGT CGGTGGGTTTGAGGCCTATGAAGGGGTGCTGCAGCTGGTGGAGGCTCGCGGGCGCTACGA GGAGCTCTGCATCGTCATGTGTGTCATCCCAGCCACCATCAGCAACAACGTCCCTGGCAC CGACTTCAGCCTGGGCTCCGACACTGCTGTAAATGCCGCCATGGAGAGCTGTGACCGCAT CAAACAGTCTGCCTCGGGGACCAAGCGCCGTGTGTTCATCGTGGAGACCATGGGGGGTTA CTGTGGCTACCTGGCCACCGTGACTGGCATTGCTGTGGGGGCCGACGCCGCCTACGTCTT CGAGGACCCTTTCAACATCCACGACTTAAAGGTCAACGTGGAGCACATGACGGAGAAGAT GAAGACAGACATTCAGAGGGGCCTGGTGCTGCGGAACGAGAAGTGCCATGACTACTACAC CACGGAGTTCCTGTACAACCTGTACTCATCAGAGGGCAAGGGCGTCTTCGACTGCAGGAC CAATGTCCTGGGCCACCTGCAGCAGGGTGGCGCTCCAACCCCCTTTGACCGGAACTATGG GACCAAGCTGGGGGTGAAGGCCATGCTGTGGTTGTCGGAGAAGCTGCGCGAGGTTTACCG CAAGGGACGGGTGTTCGCCAATGCCCCAGACTCGGCCTGCGTGATCGGCCTGAAGAAGAA GGCGGTGGCCTTCAGCCCCGTCACTGAGCTCAAGAAAGACACTGATTTCGAGCACCGCAT GCCACGGGAGCAGTGGTGGCTGAGCCTGCGGCTCATGCTGAAGATGCTGGCACAATACCG CATCAGTATGGCCGCCTACGTGTCAGGGGAGCTGGAGCACGTGACCCGCCGCACCCTGAG CATGGACAAGGGCTTCTGAGGCCAGCCATGCCCACGCCCCTCCCCAGCCCCCACCCATGC CAGCGCAGCGCCAGGGCTCAGATGGGGCCTGGGCTGTTGTGTCTGGAGCCTGCAGGCAGG TGGGGGCTGCGTCCCTGCTCAGCCCATCCCCTGCCTCTATCCCTGGCCACCTGCCAGGCC TCCCTCCGGCTGGTGTCTTGAGACCAGCCTGCCAGGCCCTCCAGCAGGAGGACAGAGTGC CCTGGGGCATCCACCTTCCTGCCCAGGGGACGTGGCGCTGTCGGTGTTTGGAGGCTGCTG CCCCCTGGCTTTGGCGCCCCATGGGCCCTCAGCGTCTCCCCATGCTGGGCTCACTACATG GGCCAGCCCTTGCTCTACCTGGCCGGTAGGCTGCTGGCGCCTAGGTTGTGTTGAGAGGGG GATGCCCCTGGCCCTGCCTCACTGTGACCTGCTCCTGCCCACGTGCAGCACCTGTCACCT TTTCTAGAAATAAAATCACCCTGACTGTGGGGTGCATCGGTCTCCGGAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAA >OriGene 5' read for NM_001002021 unedited
AGGTCGGATTTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCGTGTTTC GGCCGCCGCCATGGCCGCGGTGGACCTGGAGAAGCTGCGGGCGTCGGGCGCGGGCAAGGC CATCGGCGTCCTGACCAGCGGCGGCGACGCGCAAGGTCCCCTGACAAGCCCACCAGGCCC CCTGCTGAGATGGCTGTGACCCTGGGCTGACCCGCCCAGTGGCACATTGACTCCGCCTGG AGCTGGGGAGACCAGAGAGGCCCTGTGGTTGGACGGTGGCCTGGGTGCGCTGCTCCTGCC CTCTCCTTGCCCTGCCTCAGCTGCTGCCTGCCAGAGGCGTGGCACCTCACCTCACACCTG CTCCCTGCTGCTGAGCCCCACGCCAAGCTGGAGAGCGGATGAGAAGCATGTGTAACCAGG GTAGAGGTCGAGAGTCCTCTCGTGGGGGTCTCCATGTTCAAGGGAGCTGCCGAGGCTTGA GCAGGAGCCCCCAGCAGGAAACTGGCTTTGCCAAGGCCCCCGCTGGGACAGACTGTTTCT TTCACTGCAGTCCTGGGAGCCGAGGGCAAGGGGACAGGAAAGAGGAAGTGACCTCAGAGC CTGGTGGCACCAGCATCATGTCCAGGCTGGGGGGCATGAACGCTGCTGTCCGGGCTGTGA CGCGCATGGGCATTTATGTGGGTGCCAAAGTCTTCCTCATCTACGAGGGCTATGAGGGCC TCGTGGAGGGAGGTGAGAACATCAAGCAGGCCAACTGGCTGAGCGTCTCCAACATCATCC AGCTGGGCGGCACTATCATTGGCAGCGCTCGCTGCAGGCCTTTACCACCAGGAGGGGCGC CGGGCACGGCCTACACCTGGTCAGCACGCATCACCACCTGTGCGTCATCGGGGGGATGGC AGCTACAGTGCCACATCT |
| Restriction Sites | Please inquire |
| ACCN | NM_001002021 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001002021.1, NP_001002021.1 |
| RefSeq Size | 3402 bp |
| RefSeq ORF | 2484 bp |
| Locus ID | 5211 |
| UniProt ID | P17858 |
| Protein Families | Druggable Genome |
| Protein Pathways | Fructose and mannose metabolism, Galactose metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pentose phosphate pathway |
| Gene Summary | This gene encodes the liver (L) subunit of an enzyme that catalyzes the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate, which is a key step in glucose metabolism (glycolysis). This enzyme is a tetramer that may be composed of different subunits encoded by distinct genes in different tissues. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014] Transcript Variant: This variant (1) represents the longer transcript and encodes the shorter isoform (a). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202888 | PFKL (Myc-DDK-tagged)-Human phosphofructokinase, liver (PFKL), transcript variant 1 |
CNY 6056.00 |
|
| RC202888L3 | Lenti ORF clone of Human phosphofructokinase, liver (PFKL), transcript variant 1, Myc-DDK-tagged |
CNY 7700.00 |
|
| RC202888L4 | Lenti ORF clone of Human phosphofructokinase, liver (PFKL), transcript variant 1, mGFP tagged |
CNY 7700.00 |
|
| RG202888 | PFKL (tGFP-tagged) - Human phosphofructokinase, liver (PFKL), transcript variant 1 |
CNY 7656.00 |
