EAAT4 (SLC1A6) (NM_005071) Human Untagged Clone
CAT＃: SC125329
SLC1A6 (untagged)-Human solute carrier family 1 (high affinity aspartate/glutamate transporter), member 6 (SLC1A6)
CNY 4208.00
CNY 9120.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | EAAT4 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_005071 edited
GCACGAGCAGGGCCGGCCCGGATCCCTCCCGCAGCATAGCGCGTCCCGGCTCCGCGCCGG TGCCTCCACGGTCCGGTCCCCGCGCCGGTGCTGCACAGTCCCTGGCGGGTCCCCGCGGCC CCGGCCGGGCGCTTCGCCGGGCTCCGGCTCCTGCATCCGGGCGCAGCGCGCAGGCCGAGG CGCGGGCAGGCCGCCCCCGCCGCTCCGGACGCCGACCATGAGCAGCCATGGCAACAGCCT GTTCCTTCGGGAGAGCGGCCAGCGGCTGGGCCGGGTGGGCTGGCTGCAGCGGCTGCAGGA AAGCCTGCAGCAGAGAGCACTGCGCACGCGCCTGCGCCTGCAGACCATGACCCTCGAGCA CGTGCTGCGCTTCCTGCGCCGAAACGCCTTCATTCTGCTGACGGTCAGCGCCGTGGTCAT TGGGGTCAGCCTGGCCTTTGCCCTGCGCCCATATCAGCTCACCTACCGCCAGATCAAGTA CTTCTCTTTTCCTGGAGAGCTTCTGATGAGGATGCTGCAGATGCTGGTGTTACCTCTCAT TGTCTCCAGCCTGGTCACAGGTATGGCATCCCTGGACAACAAGGCCACGGGGCGGATGGG GATGCGGGCAGCTGTGTACTACATGGTGACCACCATCATCGCGGTCTTCATCGGCATCCT CATGGTCACCATCATCCATCCCGGGAAGGGCTCCAAGGAGGGGCTGCACCGGGAGGGCCG GATCGAGACCATCCCCACAGCTGATGCCTTCATGGACCTGATCAGAAATATGTTTCCACC AAACCTTGTGGAGGCCTGCTTCAAACAGTTCAAGACGCAGTACAGCACGAGGGTGGTAAC CAGGACCATGGTGAGGACAGAGAACGGGTCTGAGCCGGGTGCCTCCATGCCTCCTCCATT CTCAGTGGAGAACGGAACCAGCTTCCTGGAAAATGTCACTCGGGCCTTGGGTACCCTGCA GGAGATGCTGAGCTTTGAGGAGACTGTACCCGTGCCTGGCTCCGCCAATGGCATCAACGC CCTGGGCCTCGTGGTCTTCTCTGTGGCCTTTGGGCTGGTCATTGGTGGCATGAAACACAA GGGCAGAGTCCTCAGGGACTTCTTCGACAGCCTCAATGAGGCTATTATGAGGCTGGTGGG CATCATTATCTGGTATGCACCTGTGGGCATCCTGTTCCTGATTGCTGGGAAGATTCTGGA GATGGAAGACATGGCCGTCCTGGGGGGTCAGCTGGGCATGTACACCCTGACCGTCATCGT GGGCCTGTTCCTCCATGCCGGCATTGTCCTTCCCCTCATCTACTTCCTCGTCACTCACCG GAACCCCTTCCCCTTCATTGGGGGCATGCTACAAGCCCTCATCACCGCTATGGGCACGTC TTCCAGCTCGGCAACGCTGCCCATCACCTTCCGCTGCCTGGAGGAGGGCCTGGGTGTGGA CCGCCGCATCACCAGGTTCGTCCTGCCCGTGGGCGCCACGGTCAACATGGATGGCACTGC CCTCTACGAGGCCCTGGCTGCCATCTTCATTGCTCAAGTTAACAACTACGAGCTCAACCT GGGTCAGATCACAACCATCAGCATCACGGCCACAGCAGCCAGTGTTGGGGCTGCTGGCAT CCCCCAGGCGGGTCTGGTCACCATGGTCATTGTGCTTACGTCGGTCGGCTTGCCCACGGA AGACATCACGCTCATCATTGCCGTGGACTGGTTCCTTGACCGGCTTCGCACAATGACCAA CGTACTGGGGGACTCAATTGGAGCGGCCGTCATCGAGCACTTGTCTCAGCGGGAGCTGGA GCTTCAGGAAGCTGAGCTTACCCTCCCCAGCCTGGGGAAACCCTACAAGTCCCTCATGGC ACAGGAGAAGGGGGCATCCCGGGGACGGGGAGGCAACGAGAGTGCTATGTGAGGGGCCTC CAGCTCTGCCCCCCCAGAGAGGAGGGAAGGGGGCTGGGGAGGGGAGTCCTGGTGACACAT CTGTTGCCCAACTGACCGTGGGCTGAACACACGTTCTGCTTGACTCATTTAGGGGGGAGG GAAAAGTGAAATAAAGGAGCAGGAATGAAAGGTAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAA >OriGene 5' read for NM_005071 unedited
NNCGTCACCATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGCAGGGCCG GCCCGGATCCCTCCCGCAGCATAGCGCGTCCCGGCTCCGCGCCGGTGCCTCCACGGTCCG GTCCCCGCGCCGGTGCTGCACAGTCCCTGGCGGGTCCCCGCGGCCCCGGCCGGGCGCTTC GCCGGGCTCCGGCTCCTGCATCCGGGCGCAGCGCGCAGGCCGAGGCGCGGGCAGGCCGCC CCCGCCGCTCCGGACGCCGACCATGAGCAGCCATGGCAACAGCCTGTTCCTTCGGGAGAG CGGCCAGCGGCTGGGCCGGGTGGGCTGGCTGCAGCGGCTGCAGGAAAGCCTGCAGCAGAG AGCACTGCGCACGCGCCTGCGCCTGCAGACCATGACCCTCGAGCACGTGCTGCGCTTCCT GCGCCGAAACGCCTTCATTCTGCTGACGGTCAGCGCCGTGGTCATTGGGGTCAGCCTGGC CTTTGCCCTGCGCCCATATCAGCTCACCTACCGCCAGATCAAGTACTTCTCTTTTCCTGG AGAGCTTCTGATGAGGATGCTGCAGATGCTGGTGTTACCTCTCATTGTCTCCAGCCTGGT CACAGGTATGGCATCCCTGGACAACAAGGCCACGGNGCGGATGGGGATGCNGGCAGCTGT GTACTACATGGTGACCACCATCATCGCGGGTCTCATCGGCATCCTCATGGTCACCATCAT CCATCCCGGGAAGGGGCTCCAGGANGGGCTGCACCGGNAGGGCCGNATCGAGACCATCCC ACAGCTGATGCCTTCATGGACCTGATCAGAAATATGTTNCACCANACCTTGTNAGGCNCC TGCTCAACAGTCAAGACGCATCAGCACGAGGGTGGTAACCAGACATGGTGAGACANANNA CGGTCTGAGCCGGNTGCCTCATGCCTCTCATT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005071 |
| Insert Size | 2100 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005071.1, NP_005062.1 |
| RefSeq Size | 1719 bp |
| RefSeq ORF | 1695 bp |
| Locus ID | 6511 |
| UniProt ID | P48664 |
| Domains | SDF |
| Protein Families | Transmembrane |
| Gene Summary | Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:7791878). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport (By similarity). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (Probable).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR and its transcription extends past a splice site that is used in variant 1, resulting in a novel 3' coding region and 3' UTR compared to variant 1. It encodes isoform 2 which is longer and has a distinct C-terminus, compared to isoform 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221087 | SLC1A6 (Myc-DDK-tagged)-Human solute carrier family 1 (high affinity aspartate/glutamate transporter), member 6 (SLC1A6) |
CNY 4200.00 |
|
| RC221087L3 | Lenti ORF clone of Human solute carrier family 1 (high affinity aspartate/glutamate transporter), member 6 (SLC1A6), Myc-DDK-tagged |
CNY 6460.00 |
|
| RC221087L4 | Lenti ORF clone of Human solute carrier family 1 (high affinity aspartate/glutamate transporter), member 6 (SLC1A6), mGFP tagged |
CNY 6460.00 |
|
| RG221087 | SLC1A6 (tGFP-tagged) - Human solute carrier family 1 (high affinity aspartate/glutamate transporter), member 6 (SLC1A6) |
CNY 5800.00 |
