Twinkle (TWNK) (NM_021830) Human Untagged Clone
CAT＃: SC124839
C10orf2 (untagged)-Human chromosome 10 open reading frame 2 (C10orf2), transcript variant 1
CNY 5016.00
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Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ATXN8; C10orf2; IOSCA; MTDPS7; PEO; PEO1; PEOA3; PRLTS5; SANDO; SCA8; TWINL |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC124839 sequence for NM_021830 edited (data generated by NextGen Sequencing)
ATGTGGGTCCTCCTCCGAAGTGGGTACCCCCTCCGTATCTTGTTACCCCTGCGTGGGGAG TGGATGGGTCGGAGGGGCCTGCCCCGAAACTTGGCCCCAGGCCCTCCTCGCAGACGTTAC AGGAAGGAGACTCTCCAAGCCTTGGATATGCCAGTGTTGCCTGTAACTGCAACTGAAATC CGCCAGTATTTGCGGGGGCATGGGATCCCCTTCCAGGATGGTCACAGTTGCCTGCGGGCA CTGAGCCCCTTTGCAGAGTCTTCACAGCTCAAAGGCCAGACTGGTGTTACCACTTCCTTC AGCCTCTTCATTGACAAGACCACAGGCCACTTTCTCTGCATGACCAGCCTAGCAGAAGGG AGCTGGGAAGACTTCCAGGCCAGCGTGGAGGGGCGAGGGGATGGGGCCAGGGAGGGGTTT CTGCTTAGCAAGGCACCAGAATTTGAGGACAGCGAGGAGGTCCGGAGGATCTGGAACCGA GCAATACCTCTCTGGGAGCTGCCTGATCAGGAGGAGGTTCAGCTGGCTGATACAATGTTT GGCCTTACCAAGGTTACAGATGACACACTCAAGCGTTTCAGTGTGCGATATCTGCGACCT GCTCGCAGTCTTGTCTTCCCTTGGTTCTCCCCTGGGGGCTCAGGATTACGAGGCCTGAAG CTCCTAGAGGCTAAATGCCAGGGGGATGGAGTGAGCTACGAGGAAACCACTATTCCCCGA CCCAGCGCCTACCACAATCTGTTTGGATTACCACTGATTAGTCGTCGAGATGCTGAGGTG GTACTGACGAGTCGTGAGCTTGACAGCCTGGCCTTGAACCAGTCCACGGGGCTGCCTACC CTTACTCTACCCCGAGGAACGACCTGCTTACCCCCTGCCTTACTCCCTTACCTGGAACAG TTCCGGCGGATTGTATTCTGGTTGGGGGATGACCTTCGGTCCTGGGAAGCCGCCAAGTTG TTTGCACGAAAACTGAACCCCAAACGATGCTTCTTGGTGCGACCAGGAGACCAGCAACCC CGTCCCCTGGAGGCCCTGAACGGAGGCTTCAATCTTTCTCGTATTCTTCGTACCGCCCTG CCTGCCTGGCACAAGTCCATCGTATCTTTCCGGCAGCTTCGGGAGGAGGTGCTAGGAGAA CTGTCAAATGTGGAGCAAGCAGCTGGCCTCCGCTGGAGCCGCTTTCCAGACCTCAATCGT ATCTTGAAGGGACATCGAAAGGGCGAGCTGACGGTCTTCACAGGGCCAACAGGCAGTGGA AAGACGACATTCATCAGTGAGTATGCCCTGGATTTGTGTTCCCAGGGGGTGAACACACTG TGGGGTAGCTTCGAGATCAGCAATGTGAGACTAGCCCGGGTCATGCTGACACAGTTTGCC GAGGGGCGGCTGGAAGATCAACTGGACAAATATGATCACTGGGCTGACCGCTTTGAGGAC CTGCCCCTCTATTTCATGACTTTCCATGGACAGCAAAGCATCAGGACTGTAATAGATACA ATGCAACATGCAGTCTACGTCTATGACATTTGTCATGTGATCATCGACAACCTGCAGTTC ATGATGGGTCACGAGCAGCTGTCCACAGACAGGATCGCAGCTCAAGACTACATCATCGGG GTCTTTCGGAAGTTTGCAACAGACAATAACTGCCATGTGACACTGGTCATTCACCCCCGG AAAGAGGATGATGACAAGGAACTGCAGACAGCGTCCATTTTTGGCTCAGCCAAAGCAAGC CAGGAAGCAGACAATGTTCTGATCCTGCAGGACAGGAAGCTGGTAACCGGGCCAGGGAAA CGGTATCTGCAGGTGTCCAAGAACCGCTTTGATGGAGATGTAGGTGTCTTCCCGCTTGAG TTCAACAAGAACTCCCTCACCTTCTCCATTCCACCAAAGAACAAGGCCCGGCTCAAGAAG ATCAAGGATGACACTGGACCAGTGGCCAAAAAGCCCTCTTCTGGCAAAAAGGGGGCTACG ACACAGAACTCTGAGATTTGCTCAGGCCAGGCCCCCACTCCCGACCAGCCAGACACCTCC AAGCGTTCAAAGTGA Clone variation with respect to NM_021830.4 >OriGene 5' read for NM_021830 unedited
TTTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGTCATAGAGGTGAGG TGGCGNGANAGAAACTAACTAACGGACCATAGAGGTGGGGGAGCCATTGTAGAAGGACGT GGACGCGAAAGGGTCGTGTAGATGGGCATATGTGTGAAGCAGCAACGTAAAGGGGCTGAA GAGGAGAAATTCATGGAGAGAAAGAATGCACCTAGAGTGAGCTCTGCAGAGTGCTGCGTG GGATATCCCTAGAGTTTGGTCTAGTGAAGGCACGCTAACCAGGCACCTAAGGCATTTCAA GTAGTGACTTCCCACATTTGGCTAGGAATGGTGGGTCCTCTCCCGAAGTGGGTACCCCCT CCGTATCTTGTTACCCCTGGGGGGATGGATGGGGNNNNNNNNAANANNNNNNNNNNNNNN NANNNNNGNNNNNNGNNNNTNNTNNANAANNTANAGGAAGGAGANTNTNNAAGNNTTGGA TATGNNAGTGTTGGNTGTAATTGGAANTGAAATNNGNAGTATTTGGGGGGGGATGGGATN NNNTTNNAGGATGGTNAAAGTTGGNTGGGGGGATTGGGNNNNTTTGGAGAGTTTTTAAGN TTAAAGGGAAAATGGGGGTANAATTTNTTTTANNTTTTTATTGGAAAGAAAAAAGGGGNA TTTTTTTGGTTGAANAGNTTANAAAAAGGGAGNTGGGAAGAATTTNAGGNAANNTTGAAG GGGAAGGGGATGGGGGNAGGGAAGGGGTTTTTGTTTTNNAGGGANAAAATTTTAGGAAAN NAAGGAGGTNGGGAGATTTTGGAANAANAAATATTTTTTGGAANTGNTTGTAAGGAGGAG GGNNANNGGGTGTATAATGGTTGGNTTTTNAAGGTTTNAATGAAAATTTAANTTTTAGGG GGGATATTTTGAATGGTTAAAATTTA >OriGene 3' read for NM_021830 unedited
NGGGTATTGCNGAATTTTTTAGCAAAACGGNAAAAGCTCTATAAAAGTCCTGACTCCAGC AGTCTTGAGGCAGCACACAATGGCATCAGTGTCTAGGTGGCAGGCACTCCGGGCTGACAG CTTCCTTCTATGGCTCTATGACCAAGCAACCCAGCCCGAGGCAGCTCCCACCAGTGAGGC CCTGATATTGCAGGGCTAAGCACCTAGGATCCAATTCTAGTACATGCCACCTCATGCTAG GCTCTACAAAGTGGGGAGCAGAGGAGCTTGGTACCCACTGTATCACCCTTTGACTTTGAA AGCCTAGGCTGGAGTTTCGCATGAAGTCCCCGATTCCCCTCCAGGTTCATGCGGCCTGAC CTCACTGCCTTGAATCTGACCTGCTCATTCCTACCTCAGGGCTTGGATTTCCTTTTCTCT CTGCCTGGGTCCCCTTCGCCGGATGTTCACGTGGCTCTCTCCTTCTAGTTGTTTAGGCCC CAGCTCACATGCCCTCGGCTCAGAGACACCTTTTCAGACCACTTATCTACACTAGCCTGC CAGCACTCTATCGCATTTCTATTTTCTTTACAGCATTTTTCACTAAAACTAGGTTTTCTG CTAATTTGTTCATTGCTTGCTTGTCTCTTCCGCCAGAATGCATGCAGGCAGGGACTTTAT CTCTCTTTTCAGTGCTGTATATACAGGACCTCAAACAAAGCCTGAGCAACACAGTAGTTT CTCAGTAGTCTGCTACATTGAATTTTCTCACTATGGAAACCTGCTCTAGGTTAGGCCCCT CAGACTGAGAAGGGCACACAGCTCNAGACCACAGGATAGAGGAGCCCTTGCAGAGTTTTA TGCTCCAGCCTATCCTATCAGGCTCATTTCAGTGACCAGCTCTGCA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_021830 |
| Insert Size | 3600 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_021830.3, NP_068602.2 |
| RefSeq Size | 3630 bp |
| RefSeq ORF | 2055 bp |
| Locus ID | 56652 |
| UniProt ID | Q96RR1 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a hexameric DNA helicase which unwinds short stretches of double-stranded DNA in the 5' to 3' direction and, along with mitochondrial single-stranded DNA binding protein and mtDNA polymerase gamma, is thought to play a key role in mtDNA replication. The protein localizes to the mitochondrial matrix and mitochondrial nucleoids. Mutations in this gene cause infantile onset spinocerebellar ataxia (IOSCA) and progressive external ophthalmoplegia (PEO) and are also associated with several mitochondrial depletion syndromes. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Aug 2009] Transcript Variant: This variant (1) encodes the longest isoform (A; also known as Twinkle). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217014 | C10orf2 (Myc-DDK-tagged)-Human chromosome 10 open reading frame 2 (C10orf2), transcript variant 1 |
CNY 5008.00 |
|
| RC217014L1 | Lenti ORF clone of Human chromosome 10 open reading frame 2 (C10orf2), transcript variant 1, Myc-DDK-tagged |
CNY 7408.00 |
|
| RC217014L2 | Lenti ORF clone of Human chromosome 10 open reading frame 2 (C10orf2), transcript variant 1, mGFP tagged |
CNY 9120.00 |
|
| RC217014L3 | Lenti ORF clone of Human chromosome 10 open reading frame 2 (C10orf2), transcript variant 1, Myc-DDK-tagged |
CNY 9120.00 |
|
| RC217014L4 | Lenti ORF clone of Human chromosome 10 open reading frame 2 (C10orf2), transcript variant 1, mGFP tagged |
CNY 7408.00 |
|
| RG217014 | C10orf2 (tGFP-tagged) - Human chromosome 10 open reading frame 2 (C10orf2), transcript variant 1 |
CNY 6608.00 |
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